Microtubule and mitosis dynamics

CAMSAP3 Forms Dimers via its lapha-helix Domain that Directly Stabilize Non-centrosomal Microtubule Minus Ends

This study identifies how CAMSAP3 dimerizes via its C-terminal alpha-helix to stabilize non-centrosomal microtubule minus ends through enhanced CKK domain binding.

On the possibility of yet a third kinetochore system in the protist phylum Euglenozoa

This study investigates the kinetochore system in diplonemids, a group of protists, and suggests they may have evolved a previously unknown type of kinetochore.

Microtubule plus-end dynamics are tightly coupled to the turnover of the MOR1 polymerase

This study investigates MOR1's interaction with microtubule plus ends, showing its affinity for both growing and shrinking ends and its role in polymerization and depolymerization.

EB-SUN, a New Microtubule Plus-End Tracking Protein in Drosophila.

The paper identifies EB-SUN, a novel microtubule end-binding protein in Drosophila, showing its role in oocyte development and functional redundancy with EB1 during embryogenesis.

A noncanonical GTPase signaling mechanism controls exit from mitosis in budding yeast

This study reveals a noncanonical GTPase signaling mechanism in budding yeast where Tem1 localization, not its nucleotide state, controls MEN activation by increasing effective concentration for Cdc15 interaction.

The CYK-4 GAP domain regulates cortical targeting of centralspindlin to promote contractile ring assembly and facilitate ring dissolution

This study shows that the CYK-4 GAP domain recruits centralspindlin to the equatorial cortex and its catalytic activity facilitates ring dissolution during cytokinesis.

Elraglusib induces cytotoxicity via direct microtubule destabilisation independently of GSK3 inhibition

Elraglusib causes cytotoxicity by directly destabilizing microtubules, leading to mitotic arrest, DNA damage, and apoptosis, independent of GSK3 inhibition.

The response to single-gene duplication implicates translation as a key vulnerability in aneuploid yeast

The paper investigates the response to single-gene duplication in yeast, finding that translation-related genes are particularly deleterious in aneuploid cells lacking the RNA-binding protein Ssd1.

Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis

The paper investigates the distribution and interactions of gamma-tubulin complex proteins in Drosophila spermatogenesis, revealing their roles in organizing diverse MTOCs during cell organelle rearrangement.

The Saccharomyces cerevisiae cell lysis mutant cly8 is a temperature-sensitive allele of ESP1

The paper identifies the cly8 mutation as a temperature-sensitive allele of the ESP1 gene in Saccharomyces cerevisiae through whole genome sequencing and functional analysis.

Tubulin-Targeted Therapy in Melanoma Increases Cell Invasive Potential by Activation of Actomyosin Cytoskeleton - an in vitro study

This study shows that colchicine increases invasiveness in some melanoma cells by activating actomyosin, which can be counteracted by ROCK or myosin inhibitors.

CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation

The paper identifies a regulatory loop between CENP-C and Aurora B kinase mediated by the Mis12 complex, essential for correcting kinetochore-microtubule attachment errors during mitosis.

Insights into the Structural Regulation of Polo-Like Kinase Activity using AlphaFold

This paper uses AlphaFold to analyze the structural regulation of Polo-Like Kinase (PLK) activity, revealing domain arrangements and interactions that explain activity regulation and phosphopeptide selectivity.

Mitotic spindle orientation and dynamics are fine-tuned by anisotropic tension via NuMA localisation

This study shows that NuMA re-localization in response to mechanical stretch fine-tunes mitotic spindle orientation and dynamics.

Intra-manchette transport employs both microtubule and actin tracks

This study identifies F-actin as part of the manchette and reveals two distinct transport mechanisms for intra-manchette transport during spermiogenesis.

PIWIL1 is recruited to centrosomes during mitosis in Colorectal Cancer Cells and is linked to cell cycle progression.

This study shows PIWIL1 localizes to centrosomes during mitosis in CRC cells, affecting cell cycle progression and suggesting a role in stem cell maintenance.

Length-sensitive partitioning of meiotic chromosomes in Caenorhabditis elegans senses proximity and number of crossover sites

The paper investigates how C. elegans meiotic chromosomes sense length to ensure proper segregation, finding that crossover sites act as diffusion boundaries for a short arm-determining factor.

Centrosome Migration and Apical Membrane Formation in Polarized Epithelial Cells: Insights from the MDCK Cyst Model

The paper investigates the relationship between centrosome positioning, apical membrane formation, and polarity regulators in epithelial cells using MDCK cyst models, finding that centrosome-based polarity is not universal.

Mesoscale regulation of MTOCs by the E3 ligase TRIM37

This paper elucidates how TRIM37 regulates mesoscale MTOCs through a multi-step activation mechanism involving substrate recognition, oligomerization, and dimerization.

CENP-C-targeted PLK-1 regulates kinetochore function in C. elegans embryos

This study identifies CENP-C as a mediator of PLK-1 recruitment to the inner kinetochore in C. elegans embryos, revealing distinct functions for inner versus outer kinetochore PLK-1 in mitosis.

Haploidy-linked cell proliferation defects limit larval growth in Zebrafish

This study identifies mitotic defects and cell death in haploid zebrafish larvae as key factors limiting larval growth, revealing a conserved cellular mechanism across vertebrates.

An aneuploidy epistasis map reveals metabolic vulnerabilities associated with supernumerary chromosomes in cancer

This study identifies metabolic vulnerabilities in aneuploid cancer cells through CRISPR screens and multi-omic analysis, revealing reliance on pyrimidine biosynthesis and sensitivity to DNA-damaging drugs.

Increased microtubule lattice spacing correlates with selective binding of kinesin-1 in cells

The paper shows that microtubule lattice spacing affects kinesin-1 binding, with expanded lattices preferentially bound by kinesin-1.

Live imaging human embryos reveals mitotic errors and lineage specification prior to implantation

This study uses live imaging to show mitotic errors and lineage plasticity in human embryos before implantation, revealing implications for aneuploidy mosaicism.

A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function

This study identifies a phospho-regulatory module involving PPP2R3C and MAP3K1 that regulates centrosome function and is linked to congenital syndromes with gonadal dysgenesis.

A characterization of mitotic and centrosomal defects in a continuum model of Breast Cancer

This study characterizes mitotic and centrosomal defects in a breast cancer continuum model, identifying increased chromosome segregation errors and centrosome abnormalities associated with disease progression.

The microtubule regulator EFA-6 forms spatially restricted cortical foci dependent on its intrinsically disordered region and interactions with tubulins

Commitment to cytokinetic furrowing requires the coordinate activity of microtubules and Plk1

This study shows that cytokinetic furrowing requires coordinated microtubule and Plk1 activity, with commitment to furrowing occurring ~6 min post-anaphase.

A molecular switch for stress-induced activation of retrograde mitochondrial transport

The paper identifies a regulatory helix in TRAK that mediates switching between kinesin- and dynein-driven mitochondrial transport in response to stress.

Revisiting the role of the spindle assembly checkpoint in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae

The paper revisits the role of the spindle assembly checkpoint in promoting gross chromosomal rearrangements in yeast and finds no such role after correcting for experimental artifacts.