Ion channel regulation and function

Paradoxical hyperexcitability from NaV1.2 sodium channel loss in neocortical pyramidal cells

The paper shows that loss of NaV1.2 sodium channels in pyramidal neurons leads to hyperexcitability and seizures despite reduced action potential size and speed.

Structural basis of GIRK2 channel modulation by cholesterol and PIP2

The paper presents cryoEM structures of GIRK2 with and without cholesterol analog and PIP2, revealing how CHS modulates channel function by interacting with PIP2 in the transmembrane domain.

Severe deficiency of voltage-gated sodium channel NaV1.2 elevates neuronal excitability in adult mice

This study shows that severe NaV1.2 deficiency increases neuronal excitability in adult mice, challenging the current understanding of its role in autism and epilepsy.

An unconventional conductance is required for pacemaking of nigral dopamine neurons

The paper identifies an unconventional conductance essential for pacemaking in nigral dopamine neurons using pharmacological evidence and electrophysiological analysis.

Precise spatiotemporal control of voltage-gated sodium channels by photocaged saxitoxin

This study develops a photocaged saxitoxin derivative to precisely control voltage-gated sodium channel blockade and demonstrates its effects on action potential propagation in neurons.

Calbindin regulates Kv4.1 trafficking and excitability of dentate granule cells via CaMKII-dependent phosphorylation

This study shows calbindin regulates Kv4.1 trafficking and neuronal excitability via CaMKII-dependent phosphorylation, linking Ca2+ buffering to neuronal function.

Neuronal mechanism of a BK channelopathy in absence epilepsy and movement disorders

This study investigates the neuronal mechanism of a BK channelopathy in absence epilepsy and movement disorders using a knock-in mouse model and finds that BK channel inhibition can mitigate seizures and motor deficits.

REFINING THE IDENTITY AND ROLE OF KV4 CHANNELS IN MOUSE SUBSTANTIA NIGRA DOPAMINERGIC NEURONS

This study investigates the role of Kv4 channels in SNc DA neurons, showing Kv4.2 also contributes to IA and demonstrating IA gating kinetics and IH interactions determine pacemaking and post-inhibitory responses.

Somatic HCN channels augment and speed up GABAergic basket cell input-output function in human neocortex

The paper identifies a unique somatic leak current mechanism in human GABAergic basket cells that enhances signal transmission fidelity and speed compared to rodent counterparts.

Voltage-gated potassium channels ensure action potential shape fidelity in distal axons

The paper uses GEVIs to show that voltage-gated potassium channels maintain action potential shape fidelity in distal axons of hippocampal neurons.

Analysis of neuronal Ca2+ handling properties by combining perforated patch clamp recordings and the added buffer approach

This paper combines perforated patch-clamp recordings with the added buffer approach to accurately quantify neuronal Ca2+ handling properties, including both immobile and mobile buffers.

Kcns3 Deficiency Disrupts Parvalbumin Neuron Physiology in Mouse Prefrontal Cortex: Implications for the pathophysiology of schizophrenia

This study investigates the role of Kcns3 in PV neuron function using computational models and electrophysiology in Kcns3-deficient mice, finding disruptions in firing patterns and gamma oscillations.

Identification that ADAM17 mediates proteolytic maturation of calcium channel auxiliary α2δ subunits, and enables calcium current enhancement

This study identifies ADAM17 as a protease essential for proteolytic maturation of 2{delta} subunits, which enhances calcium currents and is relevant to neuropathic pain.

After-hyperpolarization promotes the firing of mitral cells through a voltage dependent modification of action potential threshold

The paper investigates how after-hyperpolarization (AHP) influences mitral cell firing by modifying action potential threshold through voltage-dependent changes in Na+ channel inactivation.

Functional microstructure of CaV-mediated calcium signaling in the axon initial segment

The paper investigates calcium channel distribution in the axon initial segment of mouse neurons and finds distinct functional roles for CaV2 and CaV3 channels.

Effects of Ih and TASK-like shunting current on dendritic impedance in layer 5 pyramidal-tract neurons

The paper investigates how HCN and TASK-like channels affect dendritic impedance in layer 5 pyramidal neurons, finding that their combination best matches experimental impedance profiles.

Spike-frequency dependent coregulation of multiple ionic conductances in fast-spiking cells forces a metabolic tradeoff

The paper shows that in electric fish electrocytes, specific ion channel transcript levels correlate with EOD frequency, and computational models reveal a metabolic tradeoff as firing rates increase.

CaSR modulates sodium channel-mediated Ca 2+ -dependent excitability

The paper investigates how extracellular calcium affects neuronal excitability and finds that the Calcium-sensing receptor (CaSR) modulates voltage-gated sodium channel (VGSC) activity, influencing action potential firing rates.

Ultrasound activates mechanosensitive TRAAK K+ channels directly through the lipid membrane

This study shows ultrasound activates TRAAK K+ channels directly through the lipid membrane, with kinetics similar to mechanical activation, suggesting mechanosensitive channels mediate ultrasound's effects on neurons.

Differential robustness to specific potassium channel deletions in midbrain dopaminergic neurons

The paper was withdrawn due to unreliable genetic deletion data in Kcnn3-/- mice, rendering the study's findings invalid.

A knock-in Drosophila model supports a conserved link between potassium channelopathy and involuntary movement

This study creates a Drosophila model with a hSlo1 D434G mutation, showing motor dysfunction and altered action potentials linked to BK channel gain-of-function.

Mechanism of hERG inhibition by gating-modifier toxin, APETx1, deduced by functional characterization

This study identifies key residues in APETx1 and hERG that mediate inhibition by stabilizing the resting state of the hERG channel.

FOXP1 negatively regulates intrinsic excitability in D2 striatal projection neurons by promoting inwardly rectifying and leak potassium currents

The paper shows that FOXP1 reduces intrinsic excitability in D2 striatal projection neurons by increasing potassium currents through transcriptional activation of KIR and KLeak channels.

Cryptic prokaryotic promoters explain instability of recombinant neuronal sodium channels in bacteria

The paper identifies cryptic prokaryotic promoters causing instability of human NaV channel cDNA in bacteria and presents stabilized plasmids for functional expression.

ATP-sensitive K+ channels control the spontaneous firing of a glycinergic interneuron in the auditory brainstem.

The paper investigates the role of ATP-sensitive K+ channels in controlling spontaneous firing of glycinergic cartwheel neurons in the auditory brainstem and links their activity to tinnitus-related hyperactivity.

Development and validation of a potent and specific inhibitor for the CLC-2 chloride channel

The paper develops and validates AK-42, a potent and specific inhibitor of CLC-2 chloride channels with nanomolar potency and high selectivity.

μ Opioid Receptors Modulate Action Potential Kinetics and Firing Frequency in Neocortical Interneurons

This study investigates how μ opioid receptors modulate action potential kinetics and firing frequency in neocortical interneurons by targeting DTX-sensitive potassium channels.

Circadian photoperiod alters TREK-1 channel function and expression in dorsal raphe serotonergic neurons

This study shows that photoperiod affects TREK-1 channel function and expression in dorsal raphe serotonergic neurons, influencing their excitability.

The Palmitoyl Acyltransferase ZDHHC14 Controls Kv1-Family Potassium Channel Clustering at the Axon Initial Segment

This study identifies ZDHHC14 as a neuronal palmitoyl acyltransferase that regulates PSD93 and Kv1 channel clustering at the axon initial segment, affecting neuronal excitability.

It takes two to tango: M-current swings with the persistent sodium current to set the speed of locomotion.

The paper identifies a persistent potassium current (IM) that interacts with the persistent sodium current (INaP) to regulate locomotion speed in neonatal rodents.