Pluripotent Stem Cells Research

This paper develops xeno-free iPSC-derived NPCs compatible with GMP for clinical use, showing long-term survival and differentiation into neurons in vivo.

The paper shows hypoxia induces an early primitive streak signature in ES cells, enhancing gastruloid elongation and lineage representation through Hif1-mediated transcriptional changes.

The paper uses fluorescence spectroscopy to track β1/2-AR membrane abundance in differentiating hiPSC-derived cardiomyocytes and compares it to mRNA levels in WT and knock-in clones.

This study develops iPSC-derived lung bud tip progenitor organoids that can be expanded and differentiated into airway or alveolar organoids, showing transcriptional similarity to in vivo progenitors.

This study maps the transcriptomic, epigenetic, and metabolic changes in rabbit preimplantation embryos, identifying novel markers of naive pluripotency and similarities to human and primate embryos.

This study maps the transcriptional changes during human pacemaker cell differentiation using single-cell RNA sequencing and identifies roles for WNT and TGFβ signaling in subtype specification.

The paper identifies H3K9me3 at the Nanog locus as a repressive mark that marks differentiation commitment and enables primitive endoderm fate acquisition in ES cells.

The paper investigates how cell cycle regulation influences transcriptional heterogeneity and lineage priming in embryonic stem cells differentiating into primitive endoderm.

The paper analyzes genomic variation in hiPSCs derived from skin and blood, finding high mutation rates in fibroblast-derived lines and frequent BCOR mutations in blood-derived lines.

The paper presents optimized protocols for generating definitive endoderm and forebrain-patterned neural organoids from mouse epiblast stem cells.

This study generates functional hepatocytes from human pluripotent stem cells using forward programming with nuclear receptors, bypassing the limitations of traditional differentiation methods.

The paper shows GATA6 and NANOG bind shared regulatory elements to drive divergent cell fates in mouse blastocysts by modulating chromatin accessibility and transcriptional activation.

This study shows pluripotency factors like OCT4 and NANOG initially repress and later enable activation of lineage specifiers, including the HoxB cluster, during early embryonic development.

This study uses base editing in bovine embryos to show that SOX2 has species-specific roles in pluripotency regulation, differing from its function in mice.

This study identifies a Nanog-dependent gene cluster that initiates epiblast specification in early mouse embryos, revealing a primary heterogeneity independent of NANOG, GATA6, and FGF signaling.

This paper applies CODEX multiplex imaging to human iPSC-derived neurons to enable high-resolution biomarker analysis.

This study compares the reprogramming dynamics of pluripotent and trophoblast stem cell states using multi-omics analysis, revealing distinct trajectories and reprogramming markers.

This study investigates how transient reprogramming affects somatic cell identity and aging in mice, finding that it can restore youthful gene expression but also suppress identity programs.

This study shows that fibronectin promotes cardiac differentiation of human pluripotent stem cells, while laminins inhibit it, and identifies fibronectin's role in early differentiation through integrin signaling.

This study shows that blocking ACTIVIN/NODAL and FGF signals in human pESCs leads to GATA3-expressing syncytia resembling trophoblasts, revealing a cell-autonomous differentiation pathway.

This study shows that PRC2 restricts naive pluripotent stem cells from differentiating into trophectoderm by maintaining bivalent promoters and H3K27me3 methylation.

This paper introduces a multi-lineage gastruloid model that integrates cardiogenesis and neurogenesis, showing interconnected cardiac and neuronal lineages in a single human model.

SyNPL uses Synthetic Notch technology to monitor and manipulate cell-cell interactions in pluripotent stem cells and embryos, enabling synthetic patterning of cell fates.

This study shows Oct4 primarily activates transcription of pluripotency enhancers rather than controlling their chromatin accessibility.

A robotic AI system using Bayesian optimization accelerates iPSC-RPE cell differentiation, achieving 88% better pigmented scores than pre-optimized methods.

This paper describes a cost-effective method to generate neuroepithelial embryoid bodies using standard well plates with anti-adherent treatment and centrifugation.

This paper develops a protocol to generate mature inflammatory astrocytes from human induced pluripotent stem cells in under 28 days, suitable for CNS disease modeling.

The paper shows that RNA Pol II pausing is essential for proper pluripotency transitions in mouse embryos by maintaining balance between gene expression states.

The paper demonstrates that geometric control of human pluripotent stem cells with BMP4 leads to efficient differentiation into hPGC-like cells, revealing NODAL signaling's critical role and improving differentiation efficiency.

This paper introduces a method to rejuvenate human fibroblasts by transient reprogramming to the maturation phase of iPSCs, followed by recovery of fibroblast identity, resulting in significant rejuvenation of transcriptome, epigenome, and functional markers.