Pluripotent Stem Cells Research

This study uses TT-seq to analyze RNA synthesis dynamics in three mouse pluripotent states, finding reduced RNA synthesis and elongation velocity in 2i and mTORi states.

This study uses thyroid hormones to mature hepatocyte-like cells derived from iPSCs, showing improved expression of adult markers and reduced fetal markers.

This study identifies specific distal enhancer-promoter associations that can reprogram MEF cells into iPS-like clones, demonstrating a method to generate pluripotent cells through 3D chromatin conformation.

This study generates craniofacial cartilage organoids from human embryonic stem cells via a neural crest cell intermediate, expressing cartilage markers and growth factors.

This study shows that BRD9 and ncBAF complexes maintain somatic cell identity and hinder reprogramming, with BRD9 inhibition increasing iPSC generation efficiency.

This paper describes a protocol to generate cortical brain organoids and optic nerve-like structures from retinal confluent cultures of pluripotent stem cells, showing transcriptional similarity to fetal cortex and functional network activity.

The paper evaluates nine computational methods for identifying and ranking reprogramming factors in directed differentiation, finding that chromatin accessibility-based methods like DeepAccess and AME perform best.

The paper compares in vitro and in vivo embryogenesis using scRNA-seq data and computational tools to evaluate stem cell differentiation protocols.

The paper identifies gene occlusion erasure as a defining difference between naive and primed pluripotency states, showing Esrrb-dependent deocclusion sustains naive pluripotency.

This study uses patient-derived organoids to identify new pathologies in individuals with PDX1 mutations, revealing intestinal and gastric metaplasia.

This study generates patient-derived induced dopaminergic neurons to show age-related autophagy impairments and alpha-synuclein accumulation in Parkinson's disease.

The paper quantifies stem cell pluripotency and differentiation states by measuring activity of developmental signaling pathways across different cell types and culture conditions.

The paper shows that RBPMS controls mRNA translation in pluripotent hESCs to preset cardiac fate competence, which is critical for cardiogenesis.

This study differentiates human pluripotent stem cells through lateral plate mesoderm to generate articular cartilage progenitors and preosteoblasts in a chemically defined protocol.

This study uses gastruloids to model embryonic blood development, identifying blood progenitor cells and their spatial localization with spatiotemporal resolution.

The paper identifies transcriptomic differences in human pluripotent stem cells that correlate with retinoic acid response and neural fate bias.

The paper identifies LIN28A as a key factor in mRNA decay during epiblast morphogenesis, linking cell identity changes to embryonic growth through nucleo-cytoplasmic translocation.

This study shows that KDM6A knockout in human iPSCs changes histone methylation at enhancers, leading to increased ectoderm gene expression.

The paper develops a cultured sensorimotor organoid model that forms functional neuromuscular junctions and uses it to study ALS subgroups.

The paper uses single-cell RNA sequencing to map transcriptional trajectories of human pluripotent stem cells differentiating into lung and hepatocyte progenitors, identifying key signaling pathways and alternative developmental paths.

This study identifies POGZ as a critical factor for maintaining embryonic stem cell pluripotency by interacting with esBAF and HP1, and shows its role in chromatin regulation and transcriptional circuitry.

This study identifies ERF as a key transcription factor that facilitates the exit from naive pluripotency by regulating enhancer activity and DNMT3B expression during the transition to primed pluripotency.

The paper generates synthetic embryo-like structures from ESCs through transcription factor-mediated reprogramming, showing implantation and decidual tissue formation.

This paper describes a platform to generate human lung buds from stem cells that mimic lung development and are susceptible to SARS-CoV-2 infection, revealing alveolar cell vulnerability and potential therapeutic targets.

The paper develops heart organoids and uses computational methods to model Ebstein's anomaly by analyzing single-cell RNA sequencing data from organoids with genetic variants.

This study identifies CTCF as a barrier to 2C-like reprogramming in ESCs, showing its role in maintaining chromatin structure and that its depletion promotes totipotent-like state conversion.

This study shows lactate promotes XEN cell differentiation in mouse embryos by enhancing glycolytic activity and lactate accumulation.

This study tests if the small molecule VC6TFZ can reprogram human PBMC into iPSCs, showing increased SSEA4 and TRA1-60 expression in treated groups.

This study shows that Oct1 works with Smad factors to activate mesodermal genes during differentiation, improving understanding of lineage specification.

This study evaluates the ability of cerebral organoids derived from APOE-edited human pluripotent stem cells to model Alzheimer's disease features, finding limited APOE effect and high variability in organoid development.