Redox biology and oxidative stress

Comprehensive elucidation of glutathione import in Escherichia coli

This study identifies the ABC transporters Gsi and Opp as essential for glutathione import into E. coli, with Gsi specifically required for oxidized glutathione uptake.

CO2 protects cells from iron-Fenton oxidative DNA damage in E. coli and humans

The paper shows CO2 protects cells from iron-Fenton oxidative DNA damage by generating carbonate radical anion, leading to guanine oxidation in both E. coli and humans.

Tsa1 is the dominant peroxide scavenger and a source of H2O2-dependent GSSG production in yeast

This study identifies Tsa1 as the primary peroxide scavenger in yeast and shows it can generate GSSG under H2O2 conditions.

Thermal Proteome Profiling reveals rapid proteomic responses to redox changes in specific cellular compartments

This study uses Thermal Proteome Profiling to identify proteomic responses to localized H2O2 production in different cellular compartments.

Enzymatic synthesis of isotopically labeled hydrogen peroxide for mass spectrometry-based applications

The paper describes a method to enzymatically synthesize isotopically labeled hydrogen peroxide (H218O2) for use in mass spectrometry to accurately measure methionine oxidation levels.

The usual and unusual functions of thioredoxins in the metabolism and stress-response of sulfate-reducing bacteria

This study investigates the roles of thioredoxins in sulfate-reducing bacteria, revealing new functions in stress response and sulfur metabolism.

Cleland Immunoblotting Unmasks Unexpected Cysteine Redox Proteoforms

The paper introduces Cleland immunoblotting, a method to detect cysteine redox proteoforms by reversing PEG-payload mobility shifts, revealing previously undetectable proteoforms in Xenopus oocytes.

Computational Analysis of Human Cysteine Redox Proteoforms Reveals Novel Insights

The paper computationally analyzes the human proteome to estimate the vast number of cysteine redox proteoforms and derives theoretical limits based on protein copy numbers and thermodynamic equilibrium.

Compensatory role of KatG in defending H2O2 stress in msr deletion strain of Salmonella Typhimurium

The study shows that the {Delta}5msr mutant of Salmonella Typhimurium upregulates KatG to compensate for the loss of Msrs, leading to increased resistance to H2O2.

Altering the redox status of Chlamydia trachomatis directly impacts its developmental cycle progression

This study shows that altering the redox status of Chlamydia trachomatis affects its developmental cycle, with AhpC overexpression delaying differentiation and knockdown accelerating it.

Fmp40 ampylase regulates cell survival upon oxidative stress by controlling Prx1 and Trx3 oxidation

This paper identifies Fmp40 as a mitochondrial AMPylase that regulates cell survival under oxidative stress by modulating the redox states of Prx1, Trx3, and Grx2.

Insights into Gastrointestinal Redox Dysregulation in a Rat Model of Alzheimer's Disease and the Assessment of the Protective Potential of D-galactose

This study investigates gut redox dysregulation in a rat Alzheimer's model and assesses D-galactose's protective effects on redox homeostasis.

The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism

The paper shows that loss of antioxidant activities in mice impairs intestinal epithelium renewal by altering lipid metabolism and stem cell function.

A novel glutathione peroxidase-based biosensor disentangles differential subcellular accumulation of H2O2 and lipid hydroperoxides

The paper develops a modified biosensor to distinguish subcellular accumulation of H2O2 and lipid hydroperoxides in plant cells under stress.

Thiol reductive stress activates the hypoxia response pathway

This study shows thiol stress activates the hypoxia response pathway in C. elegans and humans, revealing a link between reductive stress and hypoxia signaling.

CysQuant: simultaneous quantification of cysteine oxidation and protein abundance using data dependent or independent acquisition mass spectrometry

CysQuant quantifies cysteine oxidation and protein abundance using DDA or DIA-MS, revealing redox changes in Arabidopsis exposed to light.

Analysis of ageing-dependent thiol oxidation reveals early oxidation of proteins involved in core proteostasis functions

The paper analyzes protein thiol oxidation in aged yeast and creates a database for cross-species comparison of redoxome data.

Identification of chemotherapy targets reveals a nucleus-to-mitochondria ROS sensing pathway

The paper identifies a nucleus-to-mitochondria ROS sensing pathway involving CHK1 and SSBP1 that mediates resistance to platinum-based chemotherapies in ovarian cancers.

Peroxiredoxin 2 is required for the redox mediated adaptation to exercise

The paper shows that Peroxiredoxin 2 is essential for the beneficial adaptive response to exercise-induced oxidative stress in C. elegans.

Quantitative modeling of pentose phosphate pathway response to oxidative stress reveals a cooperative regulatory strategy

The paper uses metabolomics and fluxomics data to model the pentose phosphate pathway's response to oxidative stress, revealing cooperative regulatory mechanisms involving G6PD, PGI, and GAPD.

Single cell kinetic modeling of redox-based drug metabolism in head and neck squamous cell carcinoma

The paper uses single cell RNA-seq data to simulate redox responses to quinone-cycling drug treatment in HNSCC, revealing differences in antioxidant systems between cancer and healthy cells.

Duox generated reactive oxygen species activate ATR/Chk1 to induce G2 arrest in Drosophila tracheoblasts

The paper shows Duox-generated ROS activates ATR/Chk1 to induce G2 arrest in Drosophila tracheoblasts through a non-canonical mechanism.

Essential role for Ggct in erythrocyte antioxidant defense

This study shows that Ggct deletion in mice causes splenomegaly and anemia due to oxidative damage and reduced glutathione in red blood cells.

Distribution of methionine sulfoxide reductases in fungi and conservation of the free-methionine-R-sulfoxide reductase in multicellular eukaryotes

The paper surveys msr genes in 700 fungal genomes, showing conservation of MsrA, MsrB, and fRMsr in most fungi, with some exceptions and horizontal gene transfers from bacteria.

Comprehensive analyses of the cysteine thiol oxidation of PKM2 reveals the effects of multiple oxidation on cellular oxidative stress response

This study identifies oxidation-sensitive cysteine residues in PKM2 and shows their role in modulating oxidative stress response and cellular ROS levels.

Blood progenitor redox homeostasis through GABA control of TCA cycle in Drosophila hematopoiesis

The paper shows GABA regulates TCA cycle and ROS levels in Drosophila hematopoietic progenitors through PDK and Sima, maintaining metabolic homeostasis.

Structures of the germline-specific Deadhead and Thioredoxin T proteins from Drosophila melanogaster reveal unique features among Thioredoxins

The paper presents the first structures of Drosophila germline-specific Thioredoxins, Dhd and TrxT, revealing unique structural features and their potential roles in redox regulation and chromatin remodeling.

JNK signalling regulates antioxidant responses in neurons

This study shows JNK signaling enhances antioxidant defenses in neurons, protecting against oxidative stress and synapse damage in both Drosophila and mammalian models.

Unraveling Oxidative Stress Resistance: Molecular Properties Govern Proteome Vulnerability

The paper identifies molecular properties that determine oxidative stress resistance in bacteria by analyzing protein susceptibility to oxidation in Escherichia coli and Deinococcus radiodurans.

Gene Expression Signature and Molecular Mechanism of Redox Homeostasis in Colorectal Cancer

This study investigates the molecular mechanism of redox homeostasis in colorectal cancer cells, identifying how altered expression of redox-related genes supports proliferation in hypoxic conditions.