Photoreceptor and optogenetics research

A Noninvasive Approach to Optogenetics Using Focused Ultrasound Blood Brain Barrier Disruption for the Delivery of Radioluminescent Particles

This paper develops a noninvasive optogenetic system using radioluminescent particles and focused ultrasound to deliver light-sensitive proteins to brain regions without invasive procedures.

Novel rabies virus variant for bi-directional optical control reveals modulatory influence of the pulvinar on visual cortex in rat

The paper develops a novel rabies virus variant for bi-directional optical control and uses it to study the pulvinar's influence on visual cortex in rats.

A general approach to engineer positive-going eFRET voltage indicators

The paper describes engineering eFRET voltage indicators with positive-going fluorescence responses by modifying microbial rhodopsins.

Creating Red Light-Controlled Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity

The paper introduces a method to create red light-controlled protein dimerization systems with high specificity for optogenetic applications.

Forelimb movements evoked by optogenetic stimulation of the macaque motor cortex

This study demonstrates effective optogenetic stimulation of the macaque motor cortex to evoke forelimb movements and muscle activity comparable to conventional electrical stimulation.

The OptoGenBox - a device for long-term optogenetics in C. elegans

The paper introduces OptoGenBox, a device for long-term optogenetic manipulation of C. elegans, showing it reduces survival when increasing arousal or sleep deprivation.

Heterologous expression and cell membrane localization of dinoflagellate rhodopsins in mammalian cells

The paper expresses dinoflagellate rhodopsin genes in mammalian cells and shows they localize to the cell membrane, suggesting compatibility with mammalian systems and consistent with proton pump function.

Construction of light-activated neurotrophin receptors using the improved Light-Induced Dimerizer (iLID)

This paper constructs light-activated neurotrophin receptors using the iLID system and demonstrates their ability to reproduce downstream signaling in response to blue light.

Navigating the translational roadblock: Towards highly specific and effective all-optical interrogations of neural circuits

The paper improves the specificity, reduces artifacts, and optimizes stimulation efficacy in two-photon all-optical neural circuit interrogation using mouse visual cortex.

A calibrated optogenetic toolbox of stable zebrafish opsin lines

The paper develops and calibrates nine zebrafish opsin lines for reliable in vivo optogenetic control of neural activity.

Long range channelrhodopsin-assisted circuit mapping of inferior colliculus neurons with blue and red-shifted channelrhodopsins

This paper introduces a method using channelrhodopsin-assisted circuit mapping to precisely map long-range auditory projections in the brainstem with blue and red-shifted opsins.

Blue light-induced gene expression alterations in cultured neurons are the result of phototoxic interactions with neuronal culture media

This study shows blue light exposure alters gene expression in cultured neurons, but this effect is due to phototoxic interactions between light and culture media, not direct neuronal activation.

Optical activation of TrkB receptors

This paper develops optogenetic methods to activate TrkB receptors with spatial and temporal control, finding that the CRY2-based approach is most efficient.

Channelrhodopsin variants engage distinct patterns of network activity

The paper compares how different channelrhodopsin variants affect neural network activity in mice, finding distinct temporal patterns and effects on gamma-band activity.

The BioLuminescent-OptoGenetic in vivo Response to Coelenterazine is Proportional, Sensitive and Specific in Neocortex

The paper tests the relationship between bioluminescent photon emission and optogenetic effects in neocortex using BL-OG molecules and Coelenterazine doses.

Rhodopsin-based voltage imaging tools for use in excitable cells of Caenorhabditis elegans

The paper evaluates voltage imaging tools in C. elegans, showing voltage sensitivity and depolarization differences in muscles and pharynx.

Engineering strategy and vector library for the rapid generation of modular light-controlled protein-protein interactions

The paper develops a genetic engineering strategy and vector library to rapidly generate light-controlled protein-protein interactions by fusing target proteins to light-sensitive domains.

Spatiotemporal limits of optogenetic manipulations in cortical circuits

The paper benchmarks optogenetic silencers in mice to assess their spatial and temporal effects on cortical circuits, finding that photoinhibition extends beyond light spread and can produce paradoxical effects.

Wide-area all-optical neurophysiology in acute brain slices

The paper develops tools for wide-area mapping of neuronal activity in brain slices using optimized optogenetic tools and structured illumination.

Blue light induces neuronal-activity-regulated gene expression in the absence of optogenetic proteins

The paper shows blue light induces neuronal-activity-regulated gene expression in cultured mouse neurons without optogenetic proteins, suggesting blue light is unsuitable for long-term optogenetic experiments measuring transcription.

Efficient mesoscale phenotypic screening in cultured primary neuron culture

The paper develops an all-optical phenotypic screening system for cultured primary neurons to assess optogenetic actuators efficiently.

A Proof-of-Principle of Nanoscale Optogenetics

This paper demonstrates a proof-of-principle for nanoscale optogenetic activation using a focal stimulation approach with switchable light-gated channels.

Precise optical probing of perceptual detection

This study uses holographic optogenetic stimulation to show mice can detect single action potentials from small ensembles of olfactory bulb neurons, challenging previous estimates of perceptual thresholds.

Bioluminescent Multi-Characteristic Opsin for monitoring visual cortical activity upon optical stimulation

The paper develops a bioluminescent opsin (bMCO-11) to monitor visual cortical activity in mice without external excitation, enabling continuous tracking of neural responses to optical stimulation.

High-efficiency optogenetic silencing with soma-targeted anion-conducting channelrhodopsins

This paper improves optogenetic silencing by enhancing membrane targeting and subcellular compartmentalization of GtACRs, resulting in more efficient neuronal inhibition.

Modeling the Electro-chemical Properties of Microbial Opsin ChrimsonR for Application to Optogenetics-based Vision Restoration

The paper develops a model to predict ChrimsonR's electrochemical behavior under light stimulation, capturing its dynamics and adaptation mechanisms for optogenetics applications.

Reversible temporally-specific inhibition of muscle using a light-activated chloride channel

This paper demonstrates reversible, temporally specific optogenetic inhibition of motor neurons and muscle activity in mice using a light-activated chloride channel.

Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon

The paper shows that light-gated chloride channels can have excitatory effects in presynaptic terminals and introduces a targeting motif to improve their inhibitory function in somatodendritic regions.

Long-term all-optical interrogation of cortical neurons in awake-behaving non-human primates

This study develops a method for long-term all-optical interrogation of cortical neurons in awake non-human primates using optogenetics and two-photon imaging.

Parallel holographic illumination enables sub-millisecond two-photon optogenetic activation in mouse visual cortex in vivo

This paper demonstrates sub-millisecond optogenetic activation in mouse visual cortex using parallel holographic illumination, enabling precise control of neuronal activity with millisecond temporal resolution.