Photoreceptor and optogenetics research

High performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks

This paper engineers high-performance microbial opsins for precise spatial and temporal control of neuronal networks, enabling large-scale activation of defined neurons in vivo and in vitro.

In vivo characterization and application of a novel potassium channel-based optogenetic silencer in the healthy and epileptic mouse hippocampus

The paper develops and tests a novel optogenetic silencer, PACK, in healthy and epileptic mice, finding it effectively inhibits neuronal activity but causes side effects.

Single-cell in vivo brain optogenetic stimulation by two-photon excitation fluorescence transfer

This paper introduces a method to enhance two-photon optogenetic stimulation in live mouse brains by using fluorescent molecules to improve opsin activation efficiency.

A tool for the in vivo gating of gene expression in neurons using the co-occurrence of neural activity and light

The paper introduces an optogenetic tool that links neural activity to gene expression using a single AAV vector, improving temporal resolution and signal fidelity.

The trade-off between pulse duration and power in optical excitation of midbrain dopamine neurons approximates Bloch's law

The paper investigates the relationship between pulse duration and optical power in optogenetic stimulation of dopamine neurons, finding a constant product of power and duration for reward seeking.

In vivo optogenetic stimulation of the primate retina activates the visual cortex after long-term transduction

This study shows that optogenetic stimulation of the primate retina can activate the visual cortex for over 20 months, demonstrating long-term functionality and signal transmission.

All-optical electrophysiology with improved genetically encoded voltage indicators reveals interneuron network dynamics in vivo

The paper introduces improved genetically encoded voltage indicators for all-optical electrophysiology, revealing interneuron network dynamics in live mice.

AI-driven high-throughput automation of behavioral analysis and dual-channel wireless optogenetics for multiplexing brain dynamics

This paper develops a wireless platform combining AI for high-throughput behavioral analysis with dual-channel optogenetics to study brain dynamics in freely behaving rats.

Unidirectional ion transport mechanism of a light-driven chloride pump revealed using X-ray free electron lasers

This study reveals the unidirectional chloride transport mechanism in NM-R3 using TR-SFX, showing conformational changes and halide binding site insights.

Chronically implantable LED arrays for behavioral optogenetics in primates.

The paper develops a chronically implantable LED array for optogenetic perturbation in non-human primates and demonstrates its effectiveness in inducing visual deficits.

Precise optical control of gene expression in C. elegans using genetic code expansion and Cre recombinase

This study uses genetic code expansion and optical targeting in C. elegans to selectively activate gene expression in specific neurons and analyze their role in behavior.

BiPOLES: a tool for bidirectional dual-color optogenetic control of neurons

BiPOLES is an optogenetic tool that allows bidirectional control of neuronal activity using two different light colors, enabling both excitation and inhibition of the same neurons in a single experiment.

Remote control of neural function by X-ray-induced scintillation

This paper demonstrates the use of X-ray-induced scintillation to remotely control neural function in mice, achieving bidirectional modulation of behavior through optogenetic activation and inhibition.

Tissue-specific modification of cellular bioelectrical activities using the chemogenetic tool, DREADD, in zebrafish

This study demonstrates the use of DREADD to manipulate tissue-specific bioelectrical activities in zebrafish, showing voltage changes and morphological effects in melanophores, epidermis, and neurons.

Optogenetic stimulation of primate V1 reveals local laminar and large-scale cortical networks related to perceptual phosphenes

This study uses optogenetics in macaques to investigate cortical activation patterns related to visual phosphenes, revealing laminar and large-scale network responses.

A photoswitchable GPCR-based opsin for presynaptic silencing

This paper introduces parapinopsin (PPO), a photoswitchable GPCR-based opsin that enables rapid and reversible presynaptic inhibition, and demonstrates its utility in vivo.

Two-photon photoactivated voltage imaging in tissue with an Archaerhodopsin-derived reporter

The paper develops a novel genetically encoded voltage indicator, NovArch, enabling high-resolution voltage imaging in brain tissue with improved sensitivity, background rejection, and time resolution.

Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease

This paper demonstrates optogenetic delivery of trophic signals using light-activated receptors to suppress disease phenotypes in Parkinson's disease models.

Characterization of light penetration through brain tissue, for optogenetic stimulation

The paper measures light penetration through rodent brain tissue using nucleated patches and direct visualization, finding a lower extinction coefficient than previous estimates.

Optogenetic manipulation of individual or whole population Caenorhabditis elegans worms with an under hundred-dollar tool: the OptoArm

The paper introduces an affordable optogenetic device called OptoArm for manipulating C. elegans worms, showing that cholinergic neurons decline with age while GABAergic neurons and muscle cells remain resilient.

Using a bistable animal opsin for switchable and scalable optogenetic inhibition of neurons

This paper demonstrates that Lamplight, a bistable opsin, can be used for switchable and scalable optogenetic inhibition of neurons in mice.

A monogenic and fast-responding Light-Inducible Cre recombinase as a novel optogenetic switch

The paper develops LiCre, a fast-responding light-inducible Cre recombinase, and demonstrates its efficiency in yeast and human cells.

Optogenetic silencing of neurotransmitter release with a naturally occurring invertebrate rhodopsin

This paper introduces eOPN3, a modified mosquito rhodopsin that suppresses synaptic transmission with high spatiotemporal precision in vivo.

Unraveling ChR2-driven stochastic Ca2+ dynamics in astrocytes - A call for new interventional paradigms

This study develops a stochastic Ca2+ dynamics model to analyze optogenetic stimulation effects on astrocytes, identifying optimal light paradigms and parameters for enhancing Ca2+ spiking.

Optogenetic therapy: High spatiotemporal resolution and pattern recognition compatible with vision restoration in non-human primates

The paper evaluates an optogenetic construct for vision restoration in non-human primates, demonstrating high temporal resolution and visual acuity above legal blindness.

Circularly permuted LOV domain as an engineering module for optogenetic tools

This paper describes the engineering of a circularly permuted LOV domain to enable photoswitchable control of peptide C-termini, enhancing optogenetic tool capabilities.

Improved Photocleavable Proteins with Faster and More Efficient Dissociation

This paper develops improved photocleavable proteins with faster and more efficient dissociation for better optogenetic control.

Segment-Specific Optogenetic Stimulation in Drosophila melanogaster with Linear Arrays of Organic Light-Emitting Diodes

This study uses OLED arrays to deliver precise light stimuli to specific abdominal segments in Drosophila larvae, enabling controlled optogenetic activation and inhibition of sensory neurons to study crawling behavior.

Improvement of phycocyanobilin synthesis for genetically encoded phytochrome-based optogenetics.

The paper improves a PCB synthesis system for a PhyB-PIF optogenetic tool, enabling stable expression and light-inducible dimerization in cells.

State-space optimal feedback control of optogenetically driven neural activity

This paper applies state-space optimal control to feedback regulate neuronal activity in awake mice using optogenetics, showing effective single-neuron control and generalization to heterogeneous populations.