flow cytometry

Polyploid - diploid coexistence in the greater duckweed Spirodela polyrhiza

The paper examines polyploid-diploid competition in Spirodela polyrhiza under stress and shows negative frequency-dependent growth fails to overcome competitive disadvantages.

SIRPγ modulates effector differentiation of human CD8 T Cells under suboptimal TCR stimulation: implications for immune homeostasis and autoimmunity

This study shows that SIRPγ reduces effector differentiation of CD8 T cells under weak TCR signals, linking its expression to immune homeostasis and autoimmunity risk.

ImmuneFM: Pre-training Foundation Model from Cytometry Data for Immunology Research

The paper develops ImmuneFM, a foundation model pre-trained on cytometry data for immunology, showing superior performance on eight downstream tasks with limited labeled samples.

Tim-3 Promotes Early Differentiation of Tbet+ Effector T Cells During Acute Viral Infection

Tumor-infiltrating CD27-IgD- regulatory B cells suppress cytotoxic CD8+T cell responses in renal cell carcinoma

The paper identifies CD27-IgD- regulatory B cells in renal cell carcinoma that suppress CD8+T cell responses, linking them to poor prognosis.

Treatment of anti-myeloperoxidase glomerulonephritis using recombinant deoxyribonuclease I is enhanced by adeno-associated virus gene therapy

This study shows that DNase I treatment reduces ecDNA-driven inflammation in MPO-ANCA GN, and gene therapy enhances its therapeutic effect by extending its half-life.

Apolipoprotein E reduces the number and activation of non-invariant Natural Killer T cells

This study shows that ApoE deficiency, not atherosclerosis, alters non-invariant NKT cell numbers and activation, highlighting the need to use ApoE-null mice cautiously in NKT cell research.

Probabilistic migration events drive transient tissue residency of lymphocytes during homeostasis

The paper uses probabilistic modeling on a parabiosis dataset to analyze lymphocyte migration kinetics and dwell times across tissues, revealing cell type- and tissue-specific residency patterns.

Immune Responses to Salmonella Typhi Antigens among Typhoid Recovered Individuals in an Endemic Region

The paper evaluates humoral and cellular immune responses to Salmonella Typhi antigens in typhoid-recovered individuals, finding elevated IgA and TEMRA cells, and comparable IgG and memory B cells between groups.

C-C chemokine receptor 4 deficiency exacerbates early atherosclerosis in mice

This study shows that CCR4 deficiency in mice worsens early atherosclerosis by impairing Treg function and promoting proinflammatory Th1 responses.

Different CMV-specific effector T cell subtypes are associated with age, CMV serostatus, and increased systolic blood pressure

The paper examines the association between CMV-specific effector T cell subtypes, age, CMV serostatus, and systolic blood pressure in an elderly cohort.

Pulmonary macrophage subsets display distinct metabolic responses to polarising stimuli in vivo

This study investigates how pulmonary macrophage subsets respond metabolically to polarizing stimuli in vivo, revealing distinct metabolic profiles between alveolar and interstitial macrophages.

Immunomodulatory effect of HLA-G Overexpressed Mesenchymal Stromal Cell in Cell-based Therapy for Myocardial Infarction

This study shows that HLA-G1 overexpressed MSCs improve heart function after myocardial infarction by enhancing cell engraftment and modulating immune responses.

Screening of a pooled library of chimeric antigen receptor T cells based on secretory function

This paper presents a nanovial-based platform for high-throughput functional screening of CAR T cell libraries, identifying CAR constructs with distinct secretion profiles over time.

Early NK-cell and T-cell dysfunction marks progression to severe dengue in patients with obesity and healthy weight

The paper identifies early T and NK-cell dysfunction in dengue patients that predicts severe disease, with obesity exacerbating these signatures.

Machine Learning-Assisted Decoding of Temporal Transcriptional Dynamics via Fluorescent Timer

This study uses CRISPR, Tocky, and machine learning to analyze Foxp3 transcription dynamics in mice, revealing CNS2-dependent regulation and age-related expression patterns.

Lineage-Specific Immune Remodeling Following Autologous Hematopoietic Stem Cell Transplantation in Relapsing-Remitting Multiple Sclerosis

This study examines immune remodeling after aHSCT in RRMS patients, finding lineage-specific changes in T and B cells rather than full immune restoration.

Sca-1 expression depicts pro-inflammatory murine neutrophils under steady state and pathological conditions

This study identifies a novel Sca1pos neutrophil subset with pro-inflammatory properties in both steady state and inflammatory conditions.

Sealable capped nanovials for high-throughput screening of cell growth and function

This paper introduces capped nanovials as a scalable platform for single-cell and small colony culture, enabling high-throughput screening of growth and function with improved signal-to-noise ratios and co-culture capabilities.

TLR2 signaling uniquely destabilizes tumor Tregs to promote cancer immunotherapy

This study shows that TLR2 signaling in innate immune cells within tumors reduces Treg stability, enhancing cancer immunotherapy by improving CD8+ T cell function.

CMV-specific clonal expansion of Th1, GZMK⁺ CD8⁺, and TEMRA T cells revealed by human PBMC single cell profiling

The paper uses single-cell profiling to show CMV drives clonal expansion of GZMK CD8 and Th1 cells in humans.

Genital herpes shedding episodes associate with alterations in the spatial organization and activation of mucosal immune cells

This study examines how HSV-2 shedding affects immune cell organization and gene expression in the vaginal mucosa, finding increased immune surveillance and potential tissue homeostasis mechanisms.

Early disruption of the innate-adaptive immune axis in vivo after infection with virulent Georgia2007/1 ASFV

This study examines immune dynamics in pigs infected with virulent ASFV, finding early disruption of the innate-adaptive immune axis and depletion of adaptive immune cells.

A Multigenerational ''Dirty'' Mouse Model for Studying Trauma-Induced Immune Dysregulation and Infection Susceptibility

This study shows that multigenerational 'dirty' mice have more human-like immune responses to trauma and infection than clean mice, making them better models for studying immune dysfunction.

Characterisation of distinct senescent-like T cell populations during healthy and unhealthy ageing

This study identifies a distinct senescent-like CD8 TEMRA cell population in older individuals with type 2 diabetes, linked to metabolic inflammation and impaired immune function.

Cell-type specific autophagy in human leukocytes

The paper measures autophagic flux in 19 human blood cell sub-populations using flow cytometry, finding differences by cell type, sex, and age, with non-classical monocytes responding uniquely to amino acid withdrawal.

Protocol to optimize gain settings of Aurora for better data resolution

This paper describes a protocol to optimize gain settings on the Aurora platform for improved data resolution in flow cytometry.

Panel Perils: How Size, Fluorochrome Choices, and Unmixing Algorithms Shape Your Analysis Adventure!

The paper discusses challenges in ultra-high-color flow cytometry, focusing on unmixing algorithms, panel design, and fluorochrome choices impacting data analysis.

Accumulation of SA-βGal High Cells in Human Naive T Cell Compartments Reveals a Stress-Adapted, Senescent-Like State

The paper identifies and characterizes SA-βGal-high naive T cells that exhibit senescent-like features but retain proliferative capacity and cytotoxic properties, linking lysosomal-autophagic dysfunction to immune aging and inflammaging.

Differential control of mycobacteria among COVID-19 patients is associated with CD28+ CD8+ T cells

The paper examines how CD28+ CD8+ T cells in COVID-19 patients influence mycobacterial growth inhibition.