flow cytometry

Antigen-specific Th17 T cells offset the age-related decline in durable T cell immunity

Aging primarily impairs the CD8+ T cell VZV vaccine response, while effective VZV vaccination in older adults induces antigen-specific Th17 CD4+ T cells to compensate for aging defects.

Single-cell analysis of ovarian myeloid cells identifies aging associated changes in macrophages and signaling dynamics

The paper uses single-cell RNA sequencing and flow cytometry to analyze myeloid cells in young and aged mouse ovaries, identifying aging-related changes in macrophage subsets and signaling pathways.

HLA-DQ monomer-based enrichment of alloreactive CD4 T cells

This study develops an in vitro method to enrich HLA-DQ-specific CD4 T cells using HLA-DQ monomers, enhancing detection of donor-specific T cells in naive individuals.

Encoding Cell Phenotype from Label-Free Imaging Flow Cytometry with Unsupervised Deep Learning

This study uses a Variational Auto-Encoder to predict microalgal lipid content from label-free Imaging Flow Cytometry data, demonstrating a low-cost alternative to traditional methods.

Activated NK cells with a predominance of inhibitory receptors and a decidual-like phenotype expand after autologous HSCT in children with tumors.

The paper characterizes NK cell changes after autologous HSCT in children, finding an activated, decidual-like phenotype linked to cytokine levels and relapse risk.

Multiomics Reveals Compartmentalized Immune Responses and Tissue-Vascular Signatures in Lyme Disease

This study uses multiomic analysis to identify compartmentalized immune responses and tissue-vascular signatures in Lyme disease, linking local immune processes to systemic symptoms.

Targeted fusion of Antibody-Secreting Cells: unlocking monoclonal antibody production with hybridoma technology

This study improves monoclonal antibody production by targeting specific antibody-secreting cells, achieving a 100% fusion yield and over 60% mAb secretion.

Inflammatory chemokine receptors CCR1, CCR2, CCR3 and CCR5 are essential for an optimal T cell response to influenza.

This study shows that CCR1/2/3/5 deletion reduces CD4 T cell responses but not CD8 T cell responses to influenza virus infection.

Modulatory Effects of M3 Muscarinic Acetylcholine Receptor on Inflammatory Profiles of Human Memory T Helper Cells

This study shows that M3R activation in memory Th cells increases pro-inflammatory cytokines like IFN-γ and IL-17A while reducing IL-4, suggesting M3R as a target for modulating immune responses.

Physical activity modifies the metabolic profile of CD4+ and CD8+ T cell subtypes at rest and upon activation in older adults

This study shows that physical activity alters the metabolic profiles of CD4+ and CD8+ T cells in older adults, both at rest and upon activation, potentially improving T cell function.

Regulation of Leucine-Rich Repeat Kinase 2 by inflammation and IL-4

This study investigates how inflammation and IL-4 regulate LRRK2 expression and activity in immune cells, revealing cell-specific effects and the role of the LRRK2-R1441C mutation.

IL-1β Signaling Modulates T Follicular Helper and Regulatory Cells in Human Lymphoid Tissues

This study shows IL-1β signaling affects Tfh and Tfr cells in human lymphoid tissues, linking it to autoantibody production and autoimmune disease.

Marginal zone and follicular B cells respond differently to TLR4 and TLR9 stimulation

This study compares the proliferative responses of marginal zone (MZ) and follicular (FO) B cells to TLR4 (LPS) and TLR9 (CpG) stimulation, revealing distinct growth, division, and death dynamics.

Human chronic inflammation is orchestrated by spatially restricted inflammation-activated Dendritic cells

This study identifies a novel inflammation-associated dendritic cell state in human chronic inflammation and shows its spatial restriction and therapeutic relevance.

Transient lung eosinophilia during breakthrough influenza infection in vaccinated mice is associated with protective and balanced Type 1/2 immune responses.

This study shows that vaccinated mice experiencing breakthrough influenza infection have transient lung eosinophilia linked to balanced Type 1/2 immune responses without severe pathology.

A prototypical phenotype for neutrophils in lupus: the potential contribution of immune complexes

This study identifies a prototypical neutrophil phenotype in lupus patients, showing reduced viability, altered surface markers, and responsiveness to immune complexes, suggesting Fcγ receptor engagement as a key driver.

Exploring Spike-Dependent and ACE2-Independent Viral Entry into Salivary Epithelial Cells in the Absence of ACE2

This study investigates SARS-CoV-2 entry into salivary epithelial cells, finding no ACE2 expression and no spike protein binding, suggesting these cells are not major targets for infection via ACE2.

Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset

The paper analyzes immune cell changes in human pancreas-draining lymphatic tissues before and after T1D onset, identifying proinflammatory patterns and genetic risk correlations.

HO-3867 mediated modulation of Extracellular vesicles and Tumor microenvironment: A novel immunotherapeutic strategy for ovarian cancer

This study shows that HO-3867 and bevacizumab together reduce ascites, limit tumor spread, and enhance anti-tumor immunity in ovarian cancer models by modulating extracellular vesicles and immune cells.

Use of cellular FAD autofluorescence as a label-free cellular attribute for the production of chimeric antigen receptor-T cells

This paper evaluates using FAD autofluorescence as a label-free biomarker to monitor T cell activation and expansion in CAR-T cell production.

Modeling Immunosenescence on-a-chip: a platform for cancer vaccine efficacy assessment

The paper develops an in vitro platform to assess cancer vaccine efficacy in aging immune systems, showing age-dependent differences in antigen presentation and T cell responses.

Compensatory Mechanisms in γδ T Cell-Deficient Chickens Following Salmonella infection

This study shows that γδ T cells help protect chickens early against Salmonella infection, but their role is replaced by other cells later.

Targeted Irradiation and STAT3 Inhibition Reprogram the AML Microenvironment and Extend Survival: Toward Translational Immunoradiotherapy

This study combines targeted marrow irradiation with a STAT3 inhibitor to improve leukemia clearance and immune memory in AML models.

Natural killer cell function in children with severe malaria differs according to malaria transmission intensity

This study examines NK cell function in children with severe malaria in areas of low and moderate transmission, finding reduced IFN-gamma production but variable degranulation patterns.

IL-4 and TGF-β Regulate Inflammatory cytokines and Cellular Infiltration in the Lung in Mouse-adapted SARS-CoV-2 Infection

This study investigates how IL-4 and TGF-β regulate inflammation and immune cell infiltration in mouse-adapted SARS-CoV-2 infection, finding that neutralizing these cytokines alters disease pathology.

Single-cell tracking of genetically minimized Salmonella reveals mechanisms of effector gene cooperation.

This study uses genome minimization and single-cell mass cytometry to identify effector genes in Salmonella that drive pathogen dissemination between immune cells.

A Dual-Fluorescence Assay for Gene Delivery Vehicle Screening in Macrophages with an Inflammation-Inducible Reporter Construct

The paper develops a dual-fluorescence assay to screen gene delivery vehicles in macrophages using an inflammation-inducible reporter, showing divergent effects of different delivery methods.

Phenotype and function of IL-10 producing NK cells in individuals with malaria experience.

The paper shows that malaria-experienced individuals have NK cells producing more IL-10, which may reduce inflammation and protect against malaria symptoms.

γδ T Cells Target and Ablate Senescent Cells in Aging and Alleviate Pulmonary Fibrosis

This study shows γδ T cells eliminate senescent cells in aging and reduce pulmonary fibrosis by targeting senescent cells in mice and humans.

Divergent Immediate and Delayed Effects of Juvenile Exposure to Doxorubicin on the Thymus in C57BL/6 Mice

This study examines the immediate and delayed effects of juvenile doxorubicin exposure on thymic immune cell populations and gene expression in mice, revealing thymic atrophy and immunosenescence.