flow cytometry

A streamlined spectral cytometry method for FAD and NADH autofluorescence analysis in immunometabolic studies

The paper introduces a streamlined spectral cytometry method to analyze NADH and FAD autofluorescence in immune cells, demonstrating its application in assessing metabolic states of splenic B-cell subsets.

Kidney disease reprograms microbiome-host signaling to promote heart failure

This study identifies a microbiome-AhR-IL-17A axis that drives CKD-associated cardiac fibrosis and shows that AhR inhibition prevents remodeling.

Preserved CD4+ T cell helper function and coordinated antiviral immunity in people with HBV/HIV co-infection on long-term therapy

This study shows that people with HBV/HIV co-infection maintain functional HBV-specific CD4+ T helper responses and coordinated CD4/CD8 antiviral immunity, suggesting preserved antiviral immunity.

Distinct cytotoxic cell subsets underlie protective and non-protective immunity to African swine fever virus

Negative HIV serology in perinatally infected children after early treatment and viral suppression: an exploratory analysis of immune correlates

This study identifies immune correlates associated with negative HIV serology in early-treated children with HIV, showing it correlates with early ART initiation, viral suppression, and preserved immune function.

Optimization and Parallelization of Sorting by Interfacial Tension (SIFT) for High-Throughput Metabolic Cell Sorting

This paper optimizes and parallelizes the SIFT method to increase throughput and stability, applying it to study single-cell glycolysis and iron homeostasis in Jurkat cells.

Extracellular Vesicle Biomarkers in the Aqueous Humor Correlate with Retinoblastoma Seeding

The paper correlates CD133 levels in retinoblastoma aqueous humor extracellular vesicles with disease severity and seeding patterns.

The Impact of Malaria-Induced Neutrophil Subset Shift and a Link to Burkitt Lymphoma

The paper examines neutrophil subset shifts in malaria-exposed children and links these changes to Burkitt lymphoma risk in Western Kenya.

Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation

This study identifies PLA2G12A's role in Th17 differentiation through lysophospholipid signaling via extracellular vesicles, showing therapeutic potential for Th17-related diseases.

A Novel Therapeutic Approach: Gamma Secretase Inhibitor Enhances Radiotherapy and Checkpoint Blockade Therapy via Reprogramming of the Tumor Microenvironment

This study shows that combining gamma secretase inhibitor with radiotherapy and checkpoint blockade therapy reprograms the tumor microenvironment to enhance antitumor immunity.

Jump-starting the T cell response in established tumors

This study shows that entinostat enhances CD8+ T cell responses in tumors but only when administered during a specific window after T cell activation, and this effect can be restored by prior oncolytic virus treatment.

Managing Autofluorescence in Spectral Flow Cytometry for Macrophage Identification in the Liver

This study develops a spectral flow cytometry approach to identify macrophage subpopulations in healthy and MASH livers, revealing distinct autofluorescence signatures and highlighting the importance of accurate AF correction.

Organoid-T cell co-cultures functionally stratify tumor-reactive T cells and their responses to immune checkpoint inhibitors

CAR-Engineered Human Hematopoietic Stem Cell Macrophages Control Solid Tumors

This study generates CAR-engineered macrophages from human HSCs to target solid tumors, showing enhanced phagocytic activity and tumor control in vitro and in vivo.

IgE-producing cells on the move: CCR2 is a key regulator of IgE+plasma cell migration

This study identifies CCR2 as a key regulator of IgE-producing plasma cell migration by comparing their chemokine receptor profiles and migration behavior to IgG1+ cells.

Hidden Complexity of Pediatric Platelet Disorders: Functional Diversity and Unexpected Hypercoagulable Phenotypes

This study identifies functional diversity and unexpected hypercoagulable phenotypes in pediatric platelet disorders, revealing complexity beyond traditional diagnostic categories.

Comprehensive Profiling of Age- and Immune Cell- Specific Signaling Activation Using Multiplex Phosphoflow

The paper develops a multiplex phosphoflow assay to profile age- and immune cell-specific signaling activation in 18 subpopulations, revealing distinct patterns in healthy individuals aged 1-69 years.

Establishing label-free quantitative X-ray dose-response profiling by Holo-Tomographic Flow Cytometry

This paper introduces HTFC, a label-free flow cytometry method for X-ray dose-response profiling, showing high correlation with clonogenic assays and faster results.

Single-Cell Translation and Apoptosis Profiling to Define Human CD34⁺ Cell Response to Specific Factors

The paper develops a flow cytometry protocol to measure single-cell protein synthesis and apoptosis in human CD34+ cells, revealing distinct functional profiles of small molecules in hematopoietic expansion.

FlowFI: an interactive graphical software package for bespoke design of imaging parameters in flow cytometry to explore morphological diversity in bone marrow megakaryocytes

FlowFI is an open-source software tool for designing and refining imaging parameters in flow cytometry to improve the analysis of rare cell populations like bone marrow megakaryocytes.

Mechanical licensing of functional dendritic cell states for enhanced T cell priming

This paper shows that mechanical stress relaxation in dendritic cells (DCs) influences their functional states, enhancing T cell priming through improved antigen presentation and migration.

Characterization of HLA-restricted GAD65-specific CD8+ T cell responses in patients with GAD65 antibody-associated neurological disorders

This study identifies HLA-restricted CD8+ T cell responses to GAD65 peptides in patients with GAD65 antibody-associated neurological disorders, showing cytotoxic activity against GAD65-expressing cells.

Dendritic cells accelerate CAR T cells in irradiated tumors through chimeric synapses

This study shows dendritic cells enhance CAR T cell persistence in irradiated tumors by antigen-dressing and stimulating CAR T cells through chimeric receptors.

CD11c+ CD8 T cells cause IFN-g-dependent autoimmune neuroinflammation that is restrained by PD-1 signaling.

This study develops a CD8 T cell-dependent model of MS and identifies differences in brain and spinal cord inflammation, showing that IFN-g and PD-1 signaling regulate CD8 T cell-mediated neuroinflammation.

T cell dysregulation and remodeling in pediatric obesity and weight loss

This study identifies T cell dysregulation in pediatric obesity and shows that these changes persist despite weight loss.

Single-cell immune profiling of regional lymph nodes during early-stage breast cancer progression

The paper profiles immune cell composition in sentinel and axillary lymph nodes of breast cancer patients, finding T-cell exhaustion and immune changes associated with metastasis.

Reproducible detection of antigen-specific T cells and Tregs via standardized and automated activation-induced marker assay workflows

This study improves the reproducibility of antigen-specific T cell detection using standardized AIM assay workflows and automated analysis tools.

An epigenetic switch in vascular phenotype augments anti-tumor immunity

This study shows that reducing DNA methyltransferase 1 in endothelial cells enhances anti-tumor immunity by altering the tumor microenvironment and improving immune cell infiltration.

Beyond ex vivo and in vivo CAR T: antigen-driven CAR T (adCAR-T) expansion method enables rapid, physiological CAR T cells programming.

This paper introduces adCAR-T, an antigen-driven CAR T-cell expansion method that enables rapid, physiological CAR T cell programming without ex vivo manufacturing or lymphodepleting chemotherapy.

Neutrophil subsets in SLE exhibit increased glycolysis that correlates with disease activity

This study identifies increased glycolysis in SLE neutrophil subsets, correlating with disease activity and metabolic flexibility.