Microtubule and mitosis dynamics

The flavin-containing monooxygenases OSGIN1 and OSGIN2 antagonize each other to favor cytokinesis completion

The paper shows OSGIN1 and OSGIN2 antagonize each other to regulate RhoA activity and ensure cytokinesis completion.

Live dynamics of induced cell-cell fusion between mitotic and interphasic cells

This study uses cell-cell fusion to investigate how mitotic and interphase nuclei interact in shared cytoplasm, revealing checkpoint override events and plasticity in cell cycle control.

Accurate chromosome segregation is more dependent on kinetochore-bound Stu2 in meiosis than mitosis

The paper compares the role of Stu2 and Ipl1 in error correction during meiosis and mitosis in budding yeast, finding that Stu2 is more critical for accurate chromosome segregation in meiosis.

Paclitaxel induces NM2-dependent cellular contraction through GEF-H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

This study shows paclitaxel induces cancer cell contraction via GEF-H1 dissociation from microtubules and RhoA/ROCK activation, with tubulin acetylation regulating this process.

Nim1-related kinases regulate septin organization and cytokinesis by modulating Hof1 at the cell division site

This study identifies roles for Elm1 and Gin4 in septin organization and cytokinesis, revealing their interactions with Hof1 and kinase-independent functions of Hsl1 in yeast.

The CENP-A chaperone complex spatially organizes centromeres

The paper shows that the CENP-A chaperone complex forms shell-like structures around centromeres in interphase, essential for centromere architecture and genome stability.

The Greatwall/PP2A-B55α axis remodels the G2/M boundary and shapes cellular dependence on PKMYT1

The paper investigates how perturbations of the CDK1-Greatwall-PP2A-B55 feedback loop affect cell-cycle control in different cell types, finding that PP2A-B55 acts as a context-dependent G2/M repressor and that cancer cells show specific sensitivities to PKMYT1 inhibitors.

The native structure of the Trichonympha centriole cartwheel reveals a zigzag stacking pattern

The paper resolves the native structure of the Trichonympha centriole cartwheel using cryo-electron tomography, revealing a zigzag stacking pattern of SAS-6 tetramers and their role in structural stability and polarity.

STED microscopy reveals mitotic stage-dependent CENP-A organization

The paper uses STED microscopy to study CENP-A organization during mitosis in human cells, finding stage-dependent spatial variations in its structure.

Dynamic microtubules drive yolk-cytoplasm segregation in the syncytial Drosophila embryo

The paper investigates yolk-cytoplasm segregation in Drosophila embryos and identifies microtubule dynamics as the primary driver of this process.

Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division in C. elegans

The paper investigates the regulation of cortical dynein and its interaction with microtubules during mitosis in C. elegans by analyzing the effects of depleting complex components and regulators.

Absence of a spindle position checkpoint in the fungal pathogen Cryptococcus neoformans

This study shows that Cryptococcus neoformans lacks a spindle position checkpoint, allowing cytokinesis to proceed without proper chromosome segregation.

Self-propagating organelle biogenesis on synthetic beads

The paper demonstrates that recruiting a single protein to synthetic beads can seed the self-assembly of centrosome-like particles in Drosophila embryos.

Mechanism and regulation of Bim1 interactions withthe S. cerevisiae outer kinetochore Ndc80 complex

The paper identifies a conserved interaction between Bim1 and the Ndc80 complex in S. cerevisiae, showing it enhances microtubule attachment strength and is regulated by phosphorylation.

Mechanism and regulation of the Bim1 interaction withthe outer kinetochore Ndc80 complex in S. cerevisiae

This study identifies a conserved interaction between Bim1 and the Ndc80 complex in S. cerevisiae, showing it enhances microtubule attachment strength and is regulated by phosphorylation.

Spindle Assembly Checkpoint Competency Determines Sensitivity to KIF18A Inhibition in Small-Cell Lung Cancer

This study identifies SAC competency as the determinant of sensitivity to KIF18A inhibition in SCLC, offering a predictive biomarker for targeted therapies.

Stepping dynamics of dynein characterized by MINFLUX

This study uses MINFLUX to track yeast dynein stepping dynamics at high resolution, revealing ATP hydrolysis per step and 8 nm steps with transient plus-end movements.

How Phosphorylation of alpha/beta-Tubulin Perturbs Microtubule Structure: A Computational Study

This study uses molecular dynamics simulations to show how phosphorylation of alpha or beta-tubulin alters microtubule structure and affects GTP hydrolysis and dynamic instability.

Centrosome-centromere capture range, rather than centrosome arrangement, determines multipolar chromosome segregation pattern after whole-genome duplication

Kinesin-1 holoenzyme assembly coordinates cargo-adaptor recognition with heavy-chain autoinhibition

This study defines the molecular mechanisms of kinesin-1 holoenzyme assembly and cargo-adaptor regulation using KIF5C as a model system.

Prefoldin function links meiotic chromosome segregation with cellular remodeling and reveals tubulin sensitivity of the meiotic spindle

This study identifies Gim3 as a critical regulator of meiotic chromosome segregation in budding yeast, revealing its role in spindle sensitivity to tubulin levels and meiotic cellular remodeling.

Compressive force from the nuclear envelope in dividing S. pombe alters binding of mitotic spindle proteins and regulates microtubule dynamics

This study shows that compressive force from the nuclear envelope in S. pombe affects spindle biochemistry by altering microtubule dynamics and protein distribution.

Cell-state-dependent responses to PLK1 inhibition reveal a non-canonical microtubule-endolysosomal vulnerability in quiescent leukemia stem cells

This study shows that PLK1 inhibition eradicates quiescent leukemia stem cells through a non-canonical mechanism involving microtubule and endolysosomal disruption, distinct from its canonical mitotic function.

Reconstituting centriole biogenesis on an engineered cellular platform uncovers organelle assembly principles

The paper reconstitutes synthetic centrioles using an engineered platform and shows that procentriole number scales with platform surface area, revealing a mechanism for organelle number homeostasis.

Polo-like kinase phosphorylation of the orphan kinesin KIN-G negatively regulates centrin arm biogenesis in Trypanosoma brucei

This study identifies TbPLK-mediated phosphorylation of KIN-G as a negative regulator of centrin arm biogenesis in Trypanosoma brucei.

A Kinetochore-Associated Proteasome Pool Drives a Second Pathway of Cohesin Removal during Meiosis

This study identifies a proteasome function that drives centromeric cohesin removal during meiosis II independently of separase activation, revealing a dual role for the proteasome in chromosome segregation.

Elevated MPS1 converts the fibrous corona into a source of chromosomal instability in colon cancer

This study shows that elevated MPS1 causes chromosomal instability in colon cancer by converting the fibrous corona into a source of merotelic kinetochore-microtubule attachments.

Balanced RCC1 activity organizes the specialized spindle midplane during cleavage divisions

This study shows that balanced RCC1 GEF activity is essential for organizing the specialized spindle midplane during early vertebrate cleavage divisions.

Set mediates chromosome alignment and Cohesin cleavage independently of direct PP2A-B56-binding in oocyte meiosis

This study shows Set is essential for chromosome alignment and Cohesin cleavage in oocyte meiosis, functioning through Aurora B regulation and chromatin environment modulation, independent of PP2A-B56 interaction.

Chemical Interrogation and Reprogramming of ATAT1-Mediated Tubulin Acetylation

This study develops chemical tools to measure, inhibit, and visualize ATAT1-mediated tubulin acetylation, enabling controlled manipulation of microtubule stability.