Advanced Fluorescence Microscopy Techniques

A versatile platform for single fluorescent protein-based fluorescence lifetime biosensors

The paper develops a universal platform for single fluorescent protein-based FLIM biosensors capable of detecting multiple analytes with a dynamic range of 0.5 to 1.0 ns.

Autofluorescence lifetime flow cytometry with time-correlated single photon counting

This paper introduces a TCSPC-based flow cytometer for real-time single-cell autofluorescence lifetime analysis, demonstrating viability and sensitivity to metabolic changes in various cell types.

Adaptive optical third-harmonic generation microscopy for in vivo imaging of tissues

This paper introduces an adaptive optical third-harmonic generation microscope that improves in vivo imaging by correcting optical aberrations in complex tissues.

Improving positively tuned voltage indicators for faster kinetics and higher contrast

The paper improves positively tuned voltage indicators by enhancing their kinetics and contrast for better spike detection.

A Systematically Optimized Miniaturized Mesoscope (SOMM) for large-scale calcium imaging in freely moving mice

This paper introduces a miniaturized mesoscope (SOMM) for high-resolution calcium imaging in freely moving mice, achieving widefield, cellular resolution with robustness against motion artifacts.

Multiplexed in vivo imaging with fluorescence lifetime modulating tags

This paper introduces FAST tags that modulate fluorescence lifetime to enable multiplexed imaging of up to six targets in live cells and zebrafish embryos.

Tissue-specific and endogenous protein labeling with split fluorescent proteins

This paper introduces a split fluorescent protein system for tissue-specific and endogenous protein labeling in zebrafish, demonstrating its use for labeling cytoskeleton genes and manipulating protein function.

Increasing Signal Intensity of Fluorescent Oligo-Labeled Antibodies to Enable Combination Multiplexing

The paper improves fluorescent antibody labeling to enhance signal intensity for multiplex flow cytometry, showing internal labeling increases brightness and enables triple staining.

Widefield in vivo imaging system with two fluorescence and two reflectance channels, a single sCMOS detector, and shielded illumination

The paper describes a widefield in vivo imaging system for mesoscopic brain imaging, capable of capturing fluorescent and reflectance signals, and demonstrates its use in awake mice.

Large-scale deep tissue voltage imaging with targeted illumination confocal microscopy

This paper introduces a microscope combining targeted illumination and confocal gating to improve voltage imaging in dense tissues, enhancing signal-to-noise ratio and reducing crosstalk.

Low-invasive, wide-field, and cellular resolution two-photon imaging of neural population activity in brainstem and nucleus tractus solitarii

The paper develops a two-photon imaging method to study neural activity in the NTS during VNS and hormone exposure, revealing insights into vagus-NTS pathway function.

Selective illumination on line-scanning confocal mesoscope enhances background rejection in cortex-wide mouse brain imaging

The paper introduces a selective illumination method for a line-scanning confocal mesoscope to improve background rejection in in vivo mouse brain imaging.

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

This paper introduces HIF-Clear, a method for clearing and labeling FFPE brain specimens, enabling analysis of neuronal circuitry in whole brains.

CRASH2p: Closed-loop Two Photon Imaging in a Freely Moving Animal

CRASH2p enables real-time 3D motion correction for two-photon imaging in freely moving Drosophila larvae, revealing new neural correlates of behavior.

Magnetic voluntary head-fixation in transgenic rats enables lifetime imaging of hippocampal neurons

The paper introduces a magnetic voluntary head-fixation system for longitudinal imaging of hippocampal neurons in transgenic rats.

Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores

This paper introduces a method to create photochromic and blinking fluorophores by combining rhodamine and coumarin dyes, enhancing SMLM labeling options.

Clearing method adapted to FFPE tissues for 3D imaging of nerve fibers, B cells, and tertiary lymphoid structures

The paper introduces miDISCO+, a modified clearing method for FFPE tissues, enabling 3D imaging of nerve fibers, B cells, and tertiary lymphoid structures in single samples.

DeepLeMiN: Deep-learning-empowered Physics-aware Lensless Miniscope

DeepLeMiN improves lensless microscopy by using a physics-informed deep learning model to enable real-time, localized 3D imaging with high resolution.

Flat-field superresolution structured illumination microscopy with joint temporal-spatial light modulation

This paper introduces a flat-field structured illumination microscopy method to improve field uniformity and enable large-scale imaging of whole cells and pathological samples.

Nonlinear super-resolution signal processing allows intracellular tracking of calcium dynamics

This paper introduces ARES, a baseline-free method for quantifying fluorescence signals that improves spatiotemporal resolution of calcium imaging data and enables tracking of intracellular calcium dynamics.

Plant Accessible Tissue Clearing Solvent System (PATCSOS) for 3-D Imaging of Whole Plants

The paper introduces PATCSOS, a new plant tissue clearing method enabling 3-D imaging of whole plants, including Arabidopsis tissues and other species, with high efficiency and compatibility with fluorescence imaging.

Three-dimensional dipole orientation mapping with high temporal-spatial resolution using polarization modulation

This paper introduces a 3D orientation mapping microscope that achieves high temporal-spatial resolution for fluorescent dipoles, revealing molecular structure and dynamics in biological systems.

Surface passivation and functionalization for interferometric scattering microscopy

This paper describes surface passivation and functionalization methods for interferometric scattering microscopy to reduce non-specific binding and enable single-molecule experiments.

Photochromic reversion enables long-term tracking of single molecules in living plants.

This paper introduces photochromic reversion for long-term single-molecule tracking in plants and develops CASTA, a machine learning tool to analyze spatial arrest events of membrane proteins.

A blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon absorption

The paper develops T-GECO1, a blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon brightness for improved neuronal activity imaging.

Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cord in vivo

Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT

This paper introduces MIET-PAINT, a method combining DNA-PAINT and Metal-Induced Energy Transfer to achieve nanometer-scale 3D imaging with high multiplexing capability.

Open-source, high performance miniature multiphoton microscopy systems for freely behaving animals

The paper describes an open-source miniature 2-photon microscope for recording calcium dynamics in freely behaving mice during various experimental conditions.

The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples

The paper introduces an improved open-source light-sheet microscope, mesoSPIM, with enhanced resolution, throughput, and compatibility with cleared samples.

A multicolor suite for deciphering population coding in calcium and cAMP in vivo

The paper develops ultrasensitive cAMP and Ca2+ indicators to measure their dynamics in neurons and identifies their roles in signaling and behavior.