Advanced Fluorescence Microscopy Techniques

Long-Stokes-Shift mScarlet3 as a Structural Marker for Two-Photon Imaging

The paper introduces a long-Stokes-shift mScarlet variant for two-photon imaging, enabling simultaneous imaging with green sensors using a single laser.

Triplet tumbling microscopy enables in situ quantification of protein complex assembly and dynamics

The paper introduces triplet tumbling microscopy (TTM) to quantify protein complex assembly and dynamics in living cells by measuring rotational diffusion.

SMLMFlow: Improving Structural Resolution in Single Molecule Localization Microscopy with Flow Matching

SMLMFlow enhances structural resolution in single molecule localization microscopy by combining graph neural networks and hierarchical transformers with flow matching.

Fluorescence Lifetime Imaging in Plants: Practical guidelines for multiplexing, label-free imaging and data analysis

This paper establishes FLIM-Phasor as a versatile tool for multiplexing and label-free imaging in plant cells, enabling discrimination of fluorophores and endogenous fluorescence.

Versatile and scalable reflective micromirrors for single-objective light sheet microscopy

This paper introduces a tunable microfabrication method for reflective micromirrors that enhance single-objective light sheet microscopy, achieving improved signal-to-background ratio and resolution.

FluoVolt Staining Induces Photodamage During Live-Cell Voltage Imaging

This study shows FluoVolt staining and excitation cause photodamage and cell perturbation in live-cell voltage imaging, with recommendations to reduce dye concentration and loading time.

FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single-cell fluorescence lifetime data

FLIM Playground provides an interactive, open-source platform for analyzing single-cell fluorescence lifetime data, offering unified workflows and real-time analysis tools validated on cancer cell line datasets.

Fluorescence correlation spectroscopy measurements of the chlamydia outer protein B (CopB) made by cell-free protein synthesis

The paper uses fluorescence correlation spectroscopy to analyze YFP-CopB production in cell-free synthesis, measuring its concentration, size, and maturation.

Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor

The paper develops a fluorescent biosensor to measure methionine dynamics with high spatiotemporal resolution and applies it to study methionine metabolism in cells and in vivo.

Hybrid Solid-Liquid Optics Enable Scalable, High-Resolution, Multi-Immersion Light-Sheet Microscopy

This paper introduces a new optical framework and imaging devices that enable scalable, high-resolution, multi-immersion light-sheet microscopy for biological samples.

Split intein vectors permit oversize FLIM-FRET biosensor neuronal expression

The paper develops a split intein strategy to deliver oversized FLIM-FRET biosensors for neuronal cAMP monitoring in behaving mice.

Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy

This paper introduces Brightness Demixing, a method for distinguishing multiple fluorophores in SMLM by using brightness instead of spectral separation, enabling multi-target imaging in 2D and 3D.

Tracking multi-site somatic voltage dynamics via high-speed fiber photometry

This paper develops a sCMOS-based widefield imaging system for multi-site fiber photometry using soma-targeted GEVIs, demonstrating high-speed voltage tracking in mouse hippocampus and cortex.

Nanoscale visualization of ribonucleoprotein condensates with ultrastructure RNA expansion microscopy

This paper introduces UREx, an expansion microscopy method that preserves RNA and RNP structures for superresolution imaging in cells and tissues.

Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes

This paper introduces a method to measure cardiac electrophysiology using fluorescence lifetime imaging, revealing detailed voltage and calcium dynamics in cardiomyocytes.

A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo

This paper introduces VADER1, a red GEVI that enables reliable in vivo two-photon voltage imaging with millisecond resolution and supports dual-color imaging with calcium indicators.

Combining FRET and super-resolution microscopy reveals kinase activation and mitochondrial activity at the nanoscale

The paper introduces BioSenSRRF, a method combining FRET biosensors with SRRF microscopy to study AURKA activation and mitochondrial activity at the nanoscale.

Phasor EO-FLIM: Lifetime imaging with picosecond noise and 500 Hz frame rate

This paper introduces a high-speed fluorescence lifetime imaging method with picosecond noise and 500 Hz frame rate, demonstrating its application in capturing action potentials and lifetime contrast in biological samples.

Multi-fiber array-based photometry system for multi-regional functional mapping in the mouse brain

The paper introduces a multi-fiber array-based photometry system for monitoring neural activity across multiple brain regions in mice, demonstrating its utility in studying state-dependent GABAergic dynamics.

Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities

The paper introduces genetically encoded FRET sensors to detect protease activities in plants, demonstrating their use in monitoring metacaspase and Tobacco Etch Virus protease in vivo.

BrightEyes-FFS: an open-source platform for comprehensive analysis of fluorescence fluctuation spectroscopy experiments with small detector arrays

BrightEyes-FFS provides an open-source platform for analyzing fluorescence fluctuation spectroscopy data with small detector arrays, offering tools for data processing, correlation analysis, and model fitting.

Ultrasensitive voltage imaging reveals distinct electrical microdomains in neurons

The paper develops a genetically encoded voltage indicator to measure subthreshold voltage dynamics in neurons, revealing electrical diversity and local computation.

Large-scale volumetric two-photon calcium imaging enables cellular-resolution mesoscopic mapping of marmoset cortical areas

The paper develops a method for large-scale volumetric calcium imaging in marmoset brains, revealing fine-grain tuning properties and columnar organization in auditory and visual cortices.

Efficient Double Helix Detection with Steerable Filters

The paper introduces an efficient method for detecting double helix structures in 3D single-molecule microscopy using steerable filters, achieving state-of-the-art performance with minimal user input.

Bright Probes, Blurred Metabolism: Navigating Fluorescent Protein Cross-Excitation in NADH FLIM

This paper evaluates cross-talk between fluorescent proteins and NADH FLIM, identifying compatible FPs and providing a pipeline to manage cross-excitation in metabolic imaging.

A membrane-permeable small molecule biosensor accesses intractable cells and animals without genetic manipulation

The paper develops a membrane-permeable small molecule biosensor, CaMero, to detect calmodulin activation in cells and animals without genetic modification.

STIMscope: centimeter-scale all-optical imaging and patterned optogenetic manipulation at single-cell resolution

The paper introduces STIMscope, a low-cost, open-source platform for all-optical imaging and optogenetic manipulation at single-cell resolution, validated in multiple biological systems.

High frequency spike inference with particle Gibbs sampling

This paper introduces a Bayesian method using particle Gibbs sampling to infer high-frequency neuronal spikes and quantify their uncertainty, showing improved performance on benchmark datasets.

Single-molecule nanophotonic resolution of binding dynamics from apo to fully liganded for a cyclic nucleotide-gated ion channel in cell-derived vesicles

The paper uses nanophotonic methods to observe sequential ligand binding to a cyclic nucleotide-gated ion channel at single-molecule resolution, revealing cooperative binding and intermediate states.

A Data-Driven Correction Framework for Axial- and Radial-Position-Dependent Intensity Attenuation in Volumetric Fluorescence Microscopy

This paper introduces a data-driven correction framework to account for axial- and radial-position-dependent intensity attenuation in 3D fluorescence microscopy of C. elegans embryos, improving quantitative accuracy.