Glycosylation and Glycoproteins Research

N-Glycan Fingerprinting of the NIST monoclonal antibody (NISTmAb)

The paper presents methods for glycan fingerprinting of therapeutic antibodies, identifying specific glycan structures on NISTmAb.

Application of a human lectin array to rapid in vitro screening of sugars used as targeting tags for therapeutics

The paper uses a human lectin array to rapidly screen oligosaccharides for targeting specificity, validating it with existing ligands and identifying new candidates for cell-specific targeting.

Tumor- and immune-derived N-acetyl-β-D-hexosaminidase drive colorectal cancer and stratify patient risk

This study identifies N-acetyl-β-D-hexosaminidase as a driver of colorectal cancer progression and a prognostic marker in patient plasma.

Novel antibodies detect nucleocytoplasmic O-fucose in protist pathogens, cellular slime molds, and plants

This paper develops specific antibodies to detect O-fucose on nucleocytoplasmic proteins in protists, slime molds, and plants, revealing conservation of the O-fucome across species.

Functional Impact of Glycosylation and Splicing Variants of LRIG1 on EGFR Proteostasis in Cancer

The paper investigates how glycosylation and splicing variants of LRIG1 affect EGFR proteostasis in cancer, finding that N74Q mutation and exon 2 skipping enhance LRIG1-EGFR binding and degradation.

High conformational flexibility of phosphomannomutase 2: Implications for functioning mechanisms, stability and pharmacological chaperone design

This study investigates the conformational flexibility of PMM2 using structural and simulation analyses, revealing dynamic states that inform pharmacological chaperone design.

MUC2 Expression Modulates Immune Infiltration in Colorectal Cancer

This study investigates how MUC2 expression affects immune infiltration in colorectal cancer using in vitro models and CRISPR knockout, finding that MUC2 loss increases immune infiltration only in HT-29 cells.

Quantification of single molecule glycan-mannose receptor binding kinetics on myeloid cells reveals high subcellular binding heterogeneity

This paper introduces Glyco-PAINT-APP, a method to quantify single-molecule glycan-mannose receptor binding kinetics on myeloid cells, revealing subcellular binding heterogeneity.

Elucidation of the glycan structure of the b-type flagellin of Pseudomonas aeruginosa PAO1

This study identifies the unknown moiety in the b-type flagellin glycan of Pseudomonas aeruginosa PAO1 as N,N-dimethylthreonine using mass spectrometry and compares it to Type A glycan in C. difficile.

Decoding protein glycosylation by an integrative mass spectrometry-based de novo sequencing strategy

This paper develops a mass spectrometry-based method to decode glycoproteins by integrating deglycosylation and glycosylation site characterization, enabling accurate sequencing of complex glycosylated proteins.

Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner

The paper shows that cell membrane glycan content creates a kinetic barrier to viral uptake in a protein-nonspecific way by forming a physical obstacle to viral adhesion.

A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by affecting tumor associated macrophages

This study develops a nanobody-enzyme fusion protein targeting PD-L1 and sialic acid, showing anti-tumor effects by modulating tumor-associated macrophages.

Polysialylation is a general feature of immune activation

The paper shows polysialic acid (polySia) is present in immune cells and linked to activation, with sex-dependent increases in smokers.

Structural and Functional Glycosylation of the Abdala COVID-19 Vaccine

This study characterizes the N-glycan structures and their impact on the structural dynamics and functional properties of the Abdala COVID-19 vaccine.

Lectin Microarray Analysis of Salivary Gland Glycoproteins from the Arboviral Vector Aedes aegypti and the Malaria Vector Anopheles stephensi

The paper uses lectin microarrays to analyze glycoproteins in salivary glands of Aedes aegypti and Anopheles stephensi, finding similar glycan profiles with specific oligomannose and O-glycan presence.

Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryo

This study shows that reducing OGT activity in mice causes embryonic lethality proportional to the reduction in O-GlcNAc modification, with milder reductions causing developmental delays and retrovirus activation.

Chemoenzymatic synthesis of sialylated and fucosylated mucin analogs reveals glycan-dependent effects on protein conformation and degradation.

This study synthesizes sialylated and fucosylated mucin analogs to investigate glycan effects on protein conformation and degradation.

A fucose-binding superlectin from Enterobacter cloacae with high Lewis and ABO blood group antigen specificity

The paper identifies and characterizes a fucose-binding lectin from Enterobacter cloacae with high specificity for LewisA and blood group O antigens.

Quantitative site-specific glycoproteomics by ZenoTOF reveals glyco-signatures for breast cancer diagnosis

The paper uses ZenoTOF mass spectrometry to analyze glycoproteomes from breast cancer and control samples, identifying 15 site-specific glycans as potential biomarkers for breast cancer detection.

Total cell N-glycosylation is altered during neuronal differentiation of iPSC to NSC and is disturbed by trisomy 21

This study compares N-glycosylation profiles of iPSCs and NSCs from DS and non-DS individuals, finding altered glycan structures in T21 cells during neural differentiation.

Bridging Worlds: Connecting Glycan Representations with Glycoinformatics via Universal Input and a Canonicalized Nomenclature

This paper introduces a framework for automated glycan nomenclature conversion that accepts any input format and outputs a standardized IUPAC-condensed format, enabling seamless integration of glycan data.

High-resolution mastigoneme structure reveals 5',5'-phosphodiesters stabilized glycan folding

The paper reports a cryo-EM structure of Chlamydomonas mastigoneme, revealing a 5',5'-phosphodiester bond stabilizing glycan folding.

The native glycocalyx is an ordered, self-assembled hierarchical micro- and nanoarray lamellar structure conserved in evolution

This study reveals the glycocalyx as an ordered, self-assembled hierarchical micro- and nanoarray structure conserved across evolution in human and sponge cells.

Discovery and characterization of unusual O-link glycosylation of IgG4 antibody using LC-MS

The paper identifies and characterizes O-link glycosylation in IgG4 antibodies using LC-MS and mass spectrometry analysis of Dupixent.

Tumor-expressed SPPL3 supports innate anti-tumor immune responses.

This study shows that SPPL3 in tumor cells affects innate immune responses by modulating nsGSLs, reducing susceptibility to neutrophils, NK cells, and γδ T cells.

A Chemoenzymatic Strategy for Site-Specific Glyco-Tagging of Native Proteins for the Development of Biologicals

This paper develops a chemoenzymatic method to precisely glyco-tag proteins, enabling the modification of therapeutic proteins with diverse glycans while maintaining their biological activity.

OST Catalytic Subunit Redundancy Enables Therapeutic Targeting of N-Glycosylation

The paper develops OST inhibitors that selectively target STT3A and STT3B, leading to tumor regression in EGFR-mutant lung cancer models.

Cancer cell glycocalyx thickness is a druggable physical phenotype for improving cellular immunotherapy

This paper identifies glycocalyx thickness as a targetable physical phenotype and demonstrates that castanospermine reduces it, enhancing CAR-T cell efficacy against gastric cancer.

Proteome-Wide Bioinformatic Annotation and Functional Validation of the Monotopic Phosphoglycosyl Transferase Superfamily

The paper identifies and validates substrate specificity of bacterial monotopic phosphoglycosyl transferases using bioinformatics and in vitro assays, supporting inhibitor discovery for antibiotic development.

Linear and Neural Network Models for Predicting N-glycosylation in Chinese Hamster Ovary Cells Based on B4GALT Levels

The paper develops linear and neural network models to predict N-glycosylation site glycan distribution in Chinese Hamster Ovary cells using B4GALT levels, achieving a 1.39% median prediction error.