Extracellular vesicles in disease

A role for ETV1 and endothelial cell-derived extracellular vesicle microRNAs in priming fibroblast response to vesicle-bound FGF2

The paper investigates how ETV1 and endothelial cell-derived extracellular vesicle microRNAs influence fibroblast response to FGF2, showing their role in altering gene expression and function.

The intercellular transfer of extracellular vesicles markers CD63, CD9 and CD81 is spatially polarized and restricted to cell vicinity

This paper develops a coculture assay to study intercellular transfer of EV markers and finds spatially polarized transfer restricted to cell vicinity.

Extracellular Vesicles from Stem cells Rescue Cellular Phenotypes and Behavioral Deficits in SHANK3-Associated ASD Neuronal and Mouse Models

This study shows that EVs from stem cells can rescue cellular and behavioral deficits in Shank3-deficient models of ASD.

Extracellular vesicles and their RNA cargo facilitate bidirectional cross-kingdom communication between human and bacterial cells

The paper shows human and bacterial cells use extracellular vesicles and RNA cargo for bidirectional communication, affecting each other's physiology and gene expression.

Characterization of nanoparticles and fluorescent recombinant extracellular vesicles with three high-sensitivity flow cytometers

This study compares three high-sensitivity flow cytometers for analyzing nanoparticles and fluorescent extracellular vesicles using controlled conditions and different detection methods.

Molecular profiling of glioblastoma-derived extracellular vesicles identifies small nucleolar RNAs as candidate liquid biomarkers for radiation-induced senescence

This paper identifies small nucleolar RNAs (snoRNAs) as potential liquid biomarkers for radiation-induced senescence in glioblastoma by analyzing extracellular vesicle cargo changes.

Embryonic cortical extracellular vesicles confer neuroprotection via multipathway signaling with CaMKIIα as a key mediator

This study shows that embryonic cortical extracellular vesicles provide neuroprotection in vitro and in vivo through CaMKIIα-mediated signaling pathways.

ADAM10 tailors extracellular vesicles for content transfer rather than signaling by contact

The paper shows ADAM10 regulates sEV composition and signaling by cleaving receptors and sorting proteins, shifting sEVs from content delivery to contact-dependent signaling.

Engineered Extracellular Vesicles Enriched with the miR-214/199a Cluster Enhance the Efficacy of Chemotherapy for Ovarian Cancer

This paper shows engineered extracellular vesicles carrying miR-214-3p/miR-199a-5p enhance chemotherapy effectiveness in ovarian cancer by reversing chemoresistance and reprogramming tumor EV communication.

Extracellular vesicles from antigen-activated B cells stimulate oncogenic herpesvirus lytic gene expression

This study shows that B cell-derived extracellular vesicles from activated B cells stimulate lytic gene expression of oncogenic herpesviruses.

Human milk contains a heterogeneous population of EVs and microRNAs that resist simulated digestion

The paper shows human milk contains diverse extracellular vesicles that carry most microRNAs, which vary in stability during simulated infant digestion.

Exogenous Sphingomyelinase Mediates MSC-derived EV Biogenesis and Enhances Potency via Repackaging of Molecular Cargo

The paper shows that exogenous sphingomyelinase increases MSC-derived EV yield and alters their cargo, enhancing therapeutic effects in a murine wound model.

CytoLight: A Rapid and Versatile Fluorescent-Based Labeling Method for Extracellular Vesicle Characterization and Tracking

CytoLight is a fluorescent labeling method for extracellular vesicles that avoids aggregation and artifacts, offering reliable quantification and tracking in vitro and in vivo.

Obesity and Tumor Development Reprogram the Proteome and Metabolic Effects of Adipose- and Tumor-Derived Extracellular Vesicles

This study examines how obesity and tumor progression alter the proteomic composition and metabolic effects of extracellular vesicles from adipose tissue and tumors.

Measuring capture, internalization and cytosolic delivery of extracellular vesicle-embedded syntenin

This study measures the capture, internalization, and cytosolic delivery of extracellular vesicle-embedded syntenin, showing high delivery efficiency but low internalization rates.

Perfusion Bioreactor Culture Incorporating Mechanical Confinement Enhances Mesenchymal Stem Cell Extracellular Vesicle Production and Wound Healing Potential

Iterative Extracellular Vesicle Protein Co-Expression Biomarker Refinement for Preoperative Classification of Histopathological Growth Patterns in Colorectal Liver Metastasis Patients

The paper develops an iterative biomarker refinement method using extracellular vesicle protein co-expression to classify histopathological growth patterns in colorectal liver metastasis patients from plasma samples.

A549 tumorigenic and BEAS-2B non-tumorigenic cell line derived small extracellular vesicles show distinct proteomic, N-glycoproteomic and chondroitin/dermatan sulfate profiles

This study compares proteomic, N-glycoproteomic, and chondroitin/dermatan sulfate profiles of small EVs from A549 and BEAS-2B cell lines, revealing distinct molecular features associated with tumorigenicity.

PHoNUPS: Open-Source Software for Standardized Analysis and Visualization of Multi-Instrument Extracellular Vesicle Measurements

PHoNUPS is open-source software that standardizes analysis and visualization of extracellular vesicle data from multiple instruments.

Particle Phagocytosis Amplifies Secretion of Small Extracellular Vesicles in Macrophages

This study shows that phagocytosis increases small EV secretion in macrophages through a lysosome-linked mechanism, affecting both EV production and cargo.

Bioreactor-Enabled Extracellular Vesicle Production for Downstream Functional Engineering

This paper compares EV production methods using different bioreactors and shows hollow-fiber bioreactors produce higher EV concentrations with less medium, supporting efficient downstream engineering.

M1 Macrophage-Derived Small Extracellular Vesicles as Synergistic Nanotherapeutics: Harnessing Intrinsic Anticancer Activity and Drug Delivery Capacity

This study demonstrates that M1 macrophage-derived small extracellular vesicles (sEVs) act as dual-action nanotherapeutics by combining innate immune-regulatory functions with drug delivery, achieving synergistic anticancer effects.

Extracellular Particles Derived from Mesenchymal Stromal Cells Reduce Pseudomonas aeruginosa Lung Infection and Inflammation in Mice

This study shows that mesenchymal stromal cell-derived extracellular particles reduce Pseudomonas aeruginosa lung infection and inflammation in a cystic fibrosis mouse model.

Molecular Insights into the Production of Extracellular Vesicles by Plants

This study identifies proteins and pathways involved in plant EV secretion, revealing roles for EXO70, RIN4, and VAP27 in EV heterogeneity and plant immunity.

Glomerular Endothelial Cell-Derived Extracellular Vesicles Cross the Basement Membrane to Regulate Podocyte Function

This study shows glomerular endothelial cell-derived extracellular vesicles cross the basement membrane and regulate podocyte function through miR-mediated gene repression and protease activity.

Periodontal pathogen-derived extracellular vesicles promote EGFR-dependent malignant traits in human pancreatic cancer cells

This study shows that extracellular vesicles from Aggregatibacter actinomycetemcomitans promote malignant traits in human pancreatic cancer cells via EGFR-ERK/Akt signaling.

Unconventional secretion of angiogenic sonic-hedgehog-containing large extracellular vesicles is stimulated via the PI3K/Rab18-GDP pathway

The paper shows that PI3K-Rab18-GDP signaling promotes secretion of SHH-containing large extracellular vesicles from hMSCs and fibroblasts, which drive angiogenesis.

miRNAs Delivered by Prostate Cancer Extracellular Vesicles Coordinate the Regulation of Bone Metastasis

This study identifies miR-375 and miR-148a-3p in prostate cancer extracellular vesicles that promote osteoblast differentiation and tumor growth by targeting KLF4, while explaining the co-existence of osteoclastic and osteoblastic miRNAs in different metastatic phases.

Standardized brain and plasma EV enrichment pipeline validated for Single sample multi-Omic and fatty acids applications in Mouse and Human

The paper presents a standardized workflow for multi-omic profiling of brain and plasma-derived EVs from minimal human and mouse material, revealing tissue-specific and species-specific molecular signatures.

Large extracellular vesicles regulate endothelial angiogenic potential via paracrine and autocrine signaling

This study shows that large extracellular vesicles from melanoma promote angiogenic phenotypes in endothelial cells through paracrine and autocrine signaling, contributing to resistance to anti-angiogenic therapies.