Extracellular vesicles in disease

Extracellular Vesicle microRNAs From Small Airways Promote Senescence and Fibrosis in COPD

This study identifies miRNA changes in COPD small airway cells and EVs, linking them to cellular senescence and fibrosis pathways.

Serum proteomics reveals distinct phenotypic signatures to IL-6 blockade between two immunotherapies

This study compares the effects of two IL-6 inhibitors on serum extracellular vesicles using proteomics, finding distinct protein pathway alterations.

Hyperosmolar stress promotes the release of small extracellular vesicles containing metabolic proteins from corneal epithelial cells

This study shows that hyperosmolar stress increases the release of small extracellular vesicles containing metabolic proteins from corneal epithelial cells, suggesting a potential early indicator of dry eye disease.

Versatility of Campylobacter jejuni Bf extracellular vesicles in regulating adaptation and virulence under combined thermal and oxidative stress

This study shows that Campylobacter jejuni Bf extracellular vesicles help the bacteria adapt and survive under combined thermal and oxidative stress by altering membrane composition and increasing toxicity to epithelial cells.

Lack of evidence for cargo release of CD63-EVs into recipient cells

This study shows that CD63-exosomes do not fuse with recipient cell membranes to release their cargo, challenging the common belief that exosomes deliver molecular cargo via membrane fusion.

LUCID-EV: a robust and quantitative bioluminescent assay for the detection of EV cytosolic delivery in the absence of VSV-G expression

The paper introduces LUCID-EV, a bioluminescent assay to quantify EV cytosolic delivery without VSV-G, showing it occurs independently of acidic endosomes.

Anti-oxidant and anti-inflammatory Effects of Aerosolised microalgal-derived extracellular vesicles in Bronchial Epithelial-Macrophage Co-cultures at the Air-Liquid Interface

This study shows aerosolized nanoalgosomes reduce oxidative stress and inflammation in bronchial co-cultures, suggesting their potential as therapeutic agents for chronic lung diseases.

Glycoengineering of extracellular vesicles enhances cellular uptake and cargo delivery to target cells

This study shows that removing glycosaminoglycans from extracellular vesicles increases their uptake and cargo delivery to target cells, suggesting glycan structures can be tuned for better drug delivery.

Extracellular vesicles released from endothelial cells of the blood-brain barrier mediate brain Iron accumulation during LPS-induced brain Inflammation

This study shows that brain inflammation increases iron accumulation in brain tissue via extracellular vesicles (EVs) released from blood-brain barrier endothelial cells, and inhibiting EVs reduces iron accumulation.

A novel technique for monitoring Alzheimer's disease associated changes in brain-derived extracellular vesicle cargos in mouse models

This study develops an in vivo method to collect brain-derived extracellular vesicles from mouse models and identifies unique small ncRNA signatures associated with Alzheimer's disease.

A microfluidic platform for multi-marker profiling of extracellular vesicles from single-cell-derived clones

This paper introduces a microfluidic platform for multi-marker EV profiling from single-cell clones, revealing EV marker heterogeneity and EpCAM correlation with cell proliferation.

Dengue virus harnesses mosquito Syntenin to load and secrete viral RNA into salivary exosomes

This study identifies how dengue virus uses mosquito Syntenin to package and secrete viral RNA into salivary exosomes, revealing a mechanism for viral RNA incorporation into extracellular vesicles.

Combining Anion Exchange and Size Exclusion Chromatography for Extracellular Vesicle Enrichment from Small Volumes of Human and Mouse Plasma for Quantitative Proteomics

This paper develops and validates a SEC-SAX protocol for enriching extracellular vesicles from small plasma volumes for quantitative proteomics in human and mouse samples.

Cancer-derived Extracellular Vesicles for Targeted Delivery of EGFRvIII siRNA to Glioblastoma, Comparison of siRNA Loading Methods and Efficiency

This study evaluates methods for loading EGFRvIII siRNA into glioblastoma-derived extracellular vesicles, finding transfection to be the most efficient, but noting no significant tumour shrinkage in vivo.

In differentiated HL-60 neutrophil-like cells, MRP1- (ABCC1-) mediated glutathione efflux stimulated by BzATP and P2X7 receptor signalling regulates exosome release through nSMase activity

This study identifies a pathway in neutrophil-like cells where P2X7 receptor activation via BzATP triggers redox changes and nSMase activity, promoting exosome release.

Circulating extracellular vesicles drive microglial senescence and neurodegeneration in Parkinson`s disease

This study shows that circulating extracellular vesicles from Parkinson's disease patients induce microglial senescence and neurodegeneration by promoting inflammation and neuronal damage.

Brain-derived extracellular vesicle microRNAs in Lewy body and Alzheimer's disease

This study identifies miRNA biomarkers in brain-derived extracellular vesicles from plasma to distinguish Lewy body disease from Alzheimer's disease with high accuracy.

No One-Size-Fits-All: An Evidence-Based Framework to Select Plasma EV Isolation Methods

This paper evaluates 11 EV isolation methods to determine their performance and applications, providing guidance on method selection based on downstream needs.

Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts

The paper shows immune cells regulate circulating adipocyte extracellular vesicle levels by clearing EVs, with reduced clearance in obesity leading to increased circulating adipoEVs.

Engineering Extracellular Vesicle Production through Magnetic Ion Channel Activation for Bone Regeneration

This study uses Magnetic Ion Channel Activation to boost EV production from pre-osteoblasts, enhancing their osteogenic potential for bone regeneration.

Identification of a miRNA signature for schizophrenia in plasma-derived extracellular vesicles

The paper identifies a miRNA signature in plasma-derived extracellular vesicles associated with schizophrenia and links one miRNA to working memory and white matter integrity.

Engineering S. cerevisiae extracellular vesicles using synthetic biology

This paper engineers S. cerevisiae extracellular vesicles using synthetic biology to identify effective scaffold proteins for cargo loading and human protein sorting.

Macrophage-derived extracellular vesicles promote T cell-dependent inflammatory pain resolution

This study shows that macrophage-derived extracellular vesicles promote inflammatory pain resolution via T cell-dependent immune mechanisms in mice.

Human breast milk extracellular vesicles from mothers with asthma differentially modulate the release of inflammatory cytokines by primary human airway smooth muscle cells in a recipient-cell specific manner

This study shows that breast milk extracellular vesicles from mothers with asthma differentially modulate cytokine release by airway smooth muscle cells depending on the donor and recipient cell type.

Environmental cues shape extracellular vesicles biogenesis and function in Streptococcus pneumoniae

This study shows environmental factors like temperature, pH, and glucose availability influence extracellular vesicle (EV) production and function in Streptococcus pneumoniae, affecting biofilm formation and interspecies interactions.

Reference-free single-vesicle profiling of small extracellular vesicles from liquid biopsies with the PICO assay

The paper introduces PICO, a reference-free assay for single-vesicle profiling of small extracellular vesicles, demonstrating high specificity and compatibility with clinical samples.

Universal functionalization of extracellular vesicles with nanobody adapters

This paper introduces a system for functionalizing extracellular vesicles with nanobody adapters, enabling flexible and efficient attachment of target proteins without genetic engineering of cells.

Evaluation of Tetraspanins in Extracellular Vesicle Bioengineering

This study investigates the role of tetraspanins in EV bioengineering by knocking out CD9, CD63, and CD81 in HEK293T cells and shows increased engineered EV production upon reintroduction of tetraspanin-fusion constructs.

Development of a live-cell imaging assay to elucidate spatiotemporal dynamics of extracellular vesicle fusion with target cells

The paper develops a live-cell imaging assay to visualize and quantify extracellular vesicle fusion with target cells, enabling study of spatiotemporal dynamics and molecular regulators of EV fusion.

Thermal Nano-Engineering of Ginger Extracellular Vesicles for Targeted Oral Therapy of Colitis

This paper describes a thermal processing method to enhance ginger-derived extracellular vesicles for targeted oral therapy of colitis, showing improved tissue accumulation and therapeutic efficacy.