Advanced Electron Microscopy Techniques and Applications

CryoSeek identification of glycofibrils with diverse compositions and structural assemblies

The paper characterizes five new glycofibrils isolated from Tsinghua Lotus Pond using cryo-EM and AI, revealing structural diversity in their central protein components and glycans.

Cryo-EM as latent structural landscape microscopy

This paper introduces a method to analyze cryo-EM data as continuous structural landscapes, revealing molecular dynamics and previously unresolved states.

A robust workflow for 3D imaging of human mitochondria using cryo-electron tomography

The paper presents a workflow for 3D imaging of human mitochondria using cryo-electron tomography to study their structure under different conditions.

Optimizing data quality and completeness in visual proteomics experiments

The paper optimizes data quality and completeness in cryo-ET experiments by analyzing processing parameters to improve annotation and structural characterization of macromolecular complexes.

High-resolution cryo-EM structures of small protein-ligand complexes near the theoretical size limit

This study reports cryo-EM structures of two small protein-ligand complexes near the theoretical size limit of 38 kDa, demonstrating cryo-EM's utility for structure determination and drug screening.

Using Cryogenic Electron Tomography (cryoET) to Determine Rubisco Polymerization Constants in α-Carboxysomes

This paper uses cryoET to determine Rubisco polymerization constants in α-carboxysomes by converting tomography data into binding curves.

Uncompromised, multimodal, multiscale structural analysis of the hierarchically organization in mineralized tissues

The paper presents a correlative imaging workflow for multiscale structural and chemical analysis of mineralized tissues, revealing a plywood-like collagen architecture and phosphate-rich mineral platelets in zebrafish scales.

Advanced in High-Resolution Cryo Volume Electron Microscopy (cvEM) Imaging for Unicellular and Multicellular Organisms

This paper improves cryo-volume Electron Microscopy (cvEM) techniques for imaging whole cells and tissues, demonstrating enhanced resolution using Caenorhabditis elegans and Paramecium bursaria.

Image Analysis Tools for Scanning Electron Microscopy

The paper introduces image analysis tools for SEM images, available as Fiji plugins and Python scripts, for quality assessment and compatibility with custom FIB-SEM instruments.

About the alignment and 3D reconstruction of sparse cryo-scanning transmission electron tomography datasets

This paper analyzes fiducial alignment and reconstruction of sparse cryo-STET tilt-series, exploring the limits of sparse imaging for 3D reconstruction.

DM: a simple solution to suppress air-water interface interactions in cryo-EM

This paper introduces DM, a detergent that reduces air-water interface artifacts in cryo-EM, improving reconstruction quality and particle stability.

Variable Resolution Maps (VRM) in CCTBX and Phenix: Accounting For Local Resolution In cryoEM

The paper implements a method in CCTBX and Phenix to calculate variable-resolution maps from atomic models, improving accuracy by incorporating local resolution and atomic parameters.

Discovery and CryoEM Structure of FPM13, a Periplasmic Metalloprotein Unique to Francisella

The paper reports the cryoEM structure of FPM13, a novel periplasmic metalloprotein from Francisella, and shows its role in metal binding and potential detoxification.

A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM

The paper introduces SurfACT, a surfactant cocktail that reduces air-water interface artifacts in cryoEM, improving particle distribution and map completeness for four proteins.

Cryo-EM image processing of amyloid filaments in RELION-5.1

The paper introduces new tools in RELION-5.1 for automated processing and analysis of cryo-EM images of amyloid filaments, demonstrating their application on experimental datasets.

A high-performance end-to-end 3D CLEM processing workflow for facilities

This paper presents an end-to-end 3D CLEM processing pipeline that integrates existing and new tools to streamline analysis, improve reproducibility, and support facility-scale use.

In-Chamber Sublimation: A Practical Approach for Mitigating Ice and Curtaining in Cryo-Electron Tomography Lamellae Preparation

This paper demonstrates that sublimation within the SEM chamber reduces ice contamination and curtaining in cryo-ET lamellae preparation without compromising vitrification.

torch-projectors: A High-Performance Differentiable Projection Library for PyTorch

This paper introduces torch-projectors, a high-performance differentiable Fourier-space projection library for PyTorch that outperforms existing implementations by 1-2 orders of magnitude.

Mind the crack: Crack-arrest holes and soft-suspension support integration in cryo-lamella preparation for improved resistance to crack formation, fracture and deformation

This paper introduces two modifications to cryo-lamella preparation to reduce crack formation and improve mechanical resistance during imaging.

Improving the visualization of viruses in soil

This study improves virus visualization in soil using a simple spin filtration method combined with cryo-electron microscopy, revealing previously unseen morphotypes.

A foundation AI model enhances electron microscopy image analysis

DF5T improves EM image quality through five tasks, enhancing segmentation and 3D restoration of organelles.

ProPicker: Promptable Segmentation for Particle Picking in CryogenicElectron Tomography

ProPicker is a 3D segmentation model for particle picking in cryo-ET that uses prompts to detect particles efficiently and accurately.

Unravelling the Maturation Pathway of a Eukaryotic Virus through Cryo-EM

This study uses cryo-EM to determine structural models of five intermediates in the maturation pathway of Nudaurelia capensis omega virus, revealing key structural changes during viral maturation.

Absolute hand determination of glycofibrils from natural sources in cryo-EM

This study introduces Ahaha, a method for determining absolute hand of glycofibrils in cryo-EM, enabling atomic model building from natural sources.

Radiation dose effects in correlative X-ray / cryo-electron microscopy of frozen hydrated biological samples

This study evaluates the impact of X-ray radiation doses on cryo-preserved biological samples, showing that high doses do not prevent subsequent cryo-EM analysis for intermediate to high-resolution structural insights.

QuickStainer: a rapid negative staining device for improved preservation of molecular structure

QuickStainer improves preservation of labile macromolecules by rapidly staining samples with minimal incubation time on the carbon grid.

Cryogenic Electron Tomography by the Numbers: Charting Underexplored Lineages in Structural Cell Biology

This paper quantifies cryo-ET data across the biosphere to identify methodological trends, improve imaging efficiency, and highlight underexplored lineages in structural cell biology.

The vault particle is enclosed by a C13-symmetric cap with a positively charged exterior

This study presents a 2.25 Å cryo-EM structure of the vault cap, revealing a 13-fold symmetric arrangement and a positively charged exterior with implications for vault function and therapeutic applications.

Learning Mappings from Cryo-EM Images to Atomic Coordinates via Latent Representations

The paper demonstrates a method to predict atomic coordinates from cryo-EM images using a convolutional auto-encoder and regression network, achieving low RMSD in synthetic datasets.

Diffusion Probabilistic Models for Missing-Wedge Correction in Cryo-Electron Tomography

The paper introduces MW-RaMViD, a diffusion model for correcting missing-wedge artifacts in cryo-ET by generating missing tilt images, showing improved reconstruction fidelity with smaller step sizes and larger conditioning windows.