CRISPR and Genetic Engineering

A side-by-side comparison of variant function measurements using deep mutational scanning and base editing

This study compares deep mutational scanning and CRISPR base editing for variant function annotation, finding strong correlation between base editor data and gold standard DMS results.

Engineered ADARs enable single-nucleotide resolution DNA A-to-G editing without bystander effects

This paper introduces snuABE, a single-nucleotide resolution ABE that reduces bystander effects through engineered ADAR and tagRNA design, achieving precise A-to-G editing in human cells.

Random guide-independent DNA cleavage from the Argonaute of Exiguobacterium sp. AB2

This study characterizes EsAgo, a prokaryotic Argonaute from Exiguobacterium sp. AB2, showing it can cleave DNA with or without guides and has potential biotechnological applications.

SYNCAS-Mediated CRISPR-Cas9 Genome Editing in the Jewel Wasp, Nasonia vitripennis

This study uses SYNCAS to achieve efficient CRISPR-Cas9 gene editing in Nasonia vitripennis, with KO efficiencies up to ten times higher than other maternal injection methods.

Self-limiting population suppression gene drive in the West Nile vector mosquito, Culex quinquefasciatus

This paper describes a CRISPR-based gene drive targeting the doublesex gene in Culex mosquitoes to suppress populations through male-biased propagation and sterile intersexes.

Higher resolution pooled genome-wide CRISPR knockout screening in Drosophila cells using integration and anti-CRISPR (IntAC)

The paper introduces IntAC, a CRISPR screening method for Drosophila cells that improves accuracy by suppressing early Cas9 activity, leading to a more comprehensive list of fitness genes.

An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks

This paper enhances the Eco1 retron editor for precise genome editing in human cells by improving ncRNA stability and reducing sgRNA extension, achieving editing without double-strand breaks.

Rewiring DNA Repair Activity into CRISPR Signal Transduction via Synthetic DNA Transducers

This paper introduces a synthetic DNA transduction platform that links DNA repair activity to CRISPR-Cas12a activation, enabling rapid and sensitive detection of DNA glycosylase activity and inhibitor screening.

Populus VariantDB v3.2 facilitates CRISPR and Functional Genomics Research

Populus VariantDB v3.2 improves CRISPR and functional genomics research by enabling precise gRNA and primer design through querying sequence polymorphisms in poplar genomes.

A CRISPR/Cas9-based system using dual-sgRNAs for efficient gene deletion in Mycobacterium abscessus

This paper develops a CRISPR/Cas9 system with dual-sgRNAs for efficient large-fragment gene deletion in Mycobacterium abscessus, achieving over 90% deletion efficiency at certain loci.

Enhanced cleavage of genomic CCR5 using CASX2Max

The paper evaluates CasX2Max, a modified CRISPR system, showing it enhances cleavage efficiency for the CCR5 gene compared to native CasX2.

Specific Modulation of CRISPR Transcriptional Activators through RNA-Sensing Guide RNAs in Mammalian Cells and Zebrafish Embryos

This paper introduces a method to modulate CRISPR activity in response to RNA detection using engineered guide RNAs, demonstrating functionality in mammalian cells and zebrafish embryos.

Directed evolution expands CRISPR-Cas12a genome editing capacity

The paper identifies CRISPR-Cas12a variants with expanded PAM recognition, enabling targeting of ~25% of the human genome.

Benchmarking Genetic Interaction Scoring Methods for Identifying Synthetic Lethality from Combinatorial CRISPR Screens

Simply cut out -Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride

The paper develops a method for marker-free gene deletion in Trichoderma atroviride using CRISPR/Cas9 RNPs and transient telomere vectors, achieving efficient gene deletions via NHEJ.

Highly efficient genome editing in Bacillus subtilis via miniature DNA nucleases IscB.

This study develops efficient genome editing tools in Bacillus subtilis using IscB and enIscB, achieving high deletion and integration efficiencies.

Deep mutational scanning identifies variants of Cas1 and Cas2 that increase spacer acquisition in type II-A CRISPR-Cas systems

The paper uses deep mutational scanning to identify Cas1 and Cas2 variants that enhance spacer acquisition in CRISPR-Cas systems, improving immunity against phage infection.

Virulence regulates and boosts CRISPR-Cas9 immunity in Group B Streptococcus

This study shows that CovR, a virulence regulator, enhances CRISPR-Cas9 immunity in Group B Streptococcus by modulating Cas9 activity and improving immune memory.

A simplified two-plasmid system for orthogonal control of gene expression using light-activated CRISPR effector for mammalian cells

This paper simplifies the LACE optogenetic system from four to two plasmids, showing similar performance with reduced variability.

Multifaceted mRNA analysis using programmed RNA cleavage byMucilaginibacter paludis Argonaute

This paper demonstrates the use of Mpa Ago for in vitro mRNA processing and analysis, including 3' end polishing and comprehensive assessment of mRNA features using LC-MS/MS.

Temporal Control of CRISPR-Cas9 Activity Using Bio-orthogonal Chemistry.

This paper introduces a method to temporally control CRISPR-Cas9 activity using bio-orthogonal chemistry, demonstrating its effectiveness in live cells and therapeutic applications.

CRISPRware: an efficient method for contextual gRNA library design

CRISPRware designs context-specific gRNAs using sequencing data, enabling allele-specific targeting across the genome for gene therapy applications.

A compendium of nonredundant short Polymerase III promoters for CRISPR applications

The paper presents a collection of minimal Pol III promoters for CRISPR applications, showing they can match longer promoters' performance in dicots.

Visualization of a multi-turnover Cas9 after product release

This study uses guide RNA truncation and cryo-EM to visualize multi-turnover Cas9 states, revealing how PAM-proximal product binding inhibits re-binding of new targets.

Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens

The paper develops a method to quantify genetic interactions from CRISPR-Cas9 screens and identifies systematic variations in screen data.

DNA-free CRISPR Genome Editing in Raspberry (Rubus idaeus) through RNP-mediated Protoplast Transfection and Comparison of Indel Analysis Techniques

CRISPR/Cas9-mediated germline mutagenesis in the subsocial parasitoid wasp, Sclerodermus guani

This study uses CRISPR/Cas9 to achieve germline gene knockout in the subsocial wasp Sclerodermus guani, revealing a white-eye phenotype in homozygous mutants.

Visible traits demonstrate that crispant founder mice can be used for phenotypic assessment

This study shows that crispant founder mice can be used for phenotypic assessment despite genetic mosaicism, as observed null phenotypes were present in up to 100% of founders.

Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines

This study evaluates subretinal delivery of Cas9 ribonucleoproteins in mice and pigs, showing variable editing efficiency and highlighting species-specific challenges for therapeutic translation.

Structures of vertebrate R2 retrotransposon complexes during target-primed reverse transcription and after second strand nicking

The paper reports cryo-EM structures of A-clade R2 retrotransposon proteins during key steps of transgene insertion and identifies features contributing to their selectivity and function.