CRISPR and Genetic Engineering

The paper introduces MAGECS, a fast and cost-effective method to select genome-edited cells using magnetic sorting of surface antigens in Cas9-positive cells.

The paper introduces CRBR, a gene correction strategy that repairs mutations in both mitotic and post-mitotic cells, demonstrated in a mouse model of Wolcott-Rallison syndrome.

This paper converts endogenous mosquito genes into non-autonomous gene drives for malaria control by using native regulatory sequences to express antimalarial molecules and guide-RNAs.

This paper shows that CRISPR-Cas9-induced DNA breaks can lead to chromothripsis, a catastrophic chromosomal rearrangement, which is an on-target toxicity in genome editing.

The paper analyzes single-cell genomics and transcriptomics data from CRISPR-Cas9-edited human embryos to assess unintended editing outcomes and loss-of-heterozygosity.

A new genetic editing system termed intMEMOIR reveals the lineage histories of individual cells directly within their native tissue context.

This study demonstrates a method to edit mitochondrial DNA using CRISPR-Cas9 by modifying guide RNA with a stem loop element for mitochondrial targeting.

CRISPAltRations is a cloud-based tool that improves the analysis of CRISPR-induced DNA repair events by outperforming existing software in annotating indels and HDR events.

This paper describes a directed protein evolution method to enhance the catalytic efficiency of CRISPR-Cas9 variants, showing up to 4-fold improvement in AceCas9 and 2-fold increase in HDR-based gene insertion for SpyCas9.

The paper functionally analyzes regulatory sequences in C. elegans by introducing mutations and measuring their effects on gene expression and fitness.

The paper improves CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila by enhancing gRNA efficiency, enabling cell labeling, and providing genetic reagents for flexible Cas9 deployment.

The paper develops a genome-scale CRISPRi guide library for yeast, validates its coverage through fitness defects, and improves guide measurement accuracy with barcoding and in vitro transcription.

The paper proposes and models double drive gene drive designs to enable localized population genetic control by exploiting sequence differences between target and non-target populations.

This paper introduces CHYRON, a DNA recorder that writes new DNA to track lineage and record cellular stimuli, demonstrating its ability to reconstruct lineage relationships and respond to stress signals.

This paper introduces a dCas9 regulator that reduces competition among sgRNAs in CRISPRi circuits, enabling independent gene repression control.

This paper compares CRISPR-mediated HDR and PE2 systems in mice to show PE2's higher fidelity in editing a TFBS, leading to more effective tissue-specific gene inactivation.

The paper integrates two large CRISPR-Cas9 screens to improve the detection of gene dependencies and cancer vulnerabilities by addressing biases and batch effects.

This paper introduces TKIT, a CRISPR/Cas9 method for precise genomic knock-in in neurons, achieving up to 42% efficiency in mouse neurons and demonstrating its use in rat neurons for visualizing endogenous proteins.

This paper describes a programmable pAgo nuclease from Kurthia massiliensis that can cleave both DNA and RNA targets with guide flexibility and mismatch sensitivity.

This study tests split gene-drive systems in Drosophila that target essential genes, showing variable copy efficiencies and sex-biased transmission, with outcomes that can be inherently confined.

The paper optimizes CRISPR-based knockin strategies for editing multi-allelic proteins in cancer cells, demonstrating successful mutation of FOXA1 in MCF-7 cells.

This paper introduces HD-ClvR, a CRISPR-based gene drive system that eliminates resistance and localizes spread, demonstrating its effectiveness in controlling grey squirrel populations.

The paper introduces a computational pipeline to interpret CRISPR mutations in non-coding regions using epigenomic data, revealing novel regulatory elements involved in chemoresistance.

The paper identifies and engineers a compact SlugCas9 variant with broad targeting range, high specificity, and activity for genome editing.

This study uses CRISPR-Cas13a to target and downregulate BCR-ABL in CML cells, reducing downstream gene expression and cell viability.

PrimeDesign is a tool for designing prime editing guide RNAs, enabling single and combination edits, and it supports the creation of a database for over 68,500 pathogenic variants.

The paper demonstrates the use of base editors to generate precise point mutations in zebrafish for studying developmental signaling and human pathologies.

This paper uses CRISPR-Cas9 base editors in pooled screens to assess the functional impact of human variants, identifying loss-of-function mutations and drug sensitivity/resistance mutations.

This paper describes a scarless genome editing method for Drosophila using site-specific integrases and homing nucleases, enabling efficient modification near landing sites.

The paper designs a smaller genome-wide CRISPR-Cas9 library with reduced size while maintaining sensitivity and specificity, enhancing its applicability in complex models.