Escherichia coli research studies

A novel C57BL/6 mouse model for the study of severe Citrobacter rodentium infection

This paper develops a non-genetically modified C57BL/6 mouse model that develops severe Citrobacter rodentium infection, driven by microbiota changes and impaired early immunity.

MYOSIN IIA MOTOR REGULATES ATTACHING-EFFACING BACTERIA INTERACTIONS WITH INTESTINAL EPITHELIUM

This study shows that NM IIA limits A/E bacterial colonization by inhibiting actin pedestal formation, while NM IIC does not play a similar role.

Identification of specific metabolic capacities associated with major extraintestinal pathogenic Escherichia coli lineages

The paper identifies lineage-specific metabolic capacities in extraintestinal pathogenic Escherichia coli clones that may contribute to their ecological success and dissemination.

Paired in situ and molecular analyses identify mechanisms of pathogen persistence within environmental communities

The paper identifies genes and mechanisms enabling pathogen persistence in freshwater ecosystems using paired in situ and molecular analyses of Escherichia, Klebsiella, and Enterococcus.

A type III secretion system is required for Bordetella atropi host cell invasion in vivo

This study identifies the type III secretion system (T3SS) as essential for Bordetella atropi host cell invasion in vivo, with deletion of the regulator bvgS preventing invasion.

Genetic basis for antimicrobial resistance in Escherichia coli isolated from household water in municipal Ibadan, Nigeria

This study characterizes antimicrobial resistance and virulence genes in E. coli isolated from household water in Ibadan, Nigeria, finding multidrug resistance and diarrheagenic genes more common in dry season isolates.

Gut-relevant short-chain fatty acids modulate host-pathogen dynamics of uropathogenic Escherichia coli at the colonic epithelial interface

This study investigates how gut-derived short-chain fatty acids affect uropathogenic Escherichia coli behavior and host-pathogen interactions at the colonic epithelial interface.

Stitching genomics data to protein structures: Virulence factors in non-O157 Shiga toxin-producing Escherichia coli

The paper analyzes genetic variation in virulence factors of non-O157 STEC genomes and models their protein interactions to assess pathogenic potential.

New paradigm of CRISPR spacerome for classification of global Escherichia coli lineages

The paper identifies a conserved CRISPR spacerome signature across globally distributed E. coli strains, suggesting its potential as a marker for epidemiological tracking.

Decoding E. coli's Gut Survival Strategies: A CRISPRi Approach Across Diets, Inflammatory Environment and Strains

This study uses CRISPRi to identify genetic factors affecting E. coli adaptation across diets, inflammation, and strains in a mouse model.

Degradation of the intestinal mucus layer by the ETEC protease EatA is species specific determined by the structure of the MUC2 mucin

The paper shows ETEC protease EatA degrades human MUC2 mucin specifically, enabling bacterial infection by disrupting the intestinal mucus layer.

Oligomeric coiled-coil adhesins that drive chain-like adhesion diversify surface colonization strategies in Shiga toxin-producing Escherichia coli

This study investigates CLAP in LEE-negative STEC, showing chain formation dynamics and genetic diversity, with claB prevalence and EibG's role in virulence.

centriflaken: an automated data analysis pipeline for assembly and in silico analyses of foodborne pathogens from metagenomic samples

centriflaken automates metagenomic analysis for detecting and characterizing foodborne pathogens like STEC, reducing analysis time and effort.

Accurate interpretation of within-host dissemination using barcoded bacteria

This paper develops a simulation-based metric to accurately interpret bacterial dissemination by distinguishing true dissemination from barcode confounding in barcoded bacteria studies.

Protective and pathogenic antibody responses from a primate Shigella outbreak inform vaccine design

The paper isolates antibodies from a Shigella outbreak in non-human primates, showing some target O-antigen and T3SS proteins, with some antibodies enhancing virulence.

Scaling-up the production of recombinant EIT antigen by E. coli fermentation for a vaccine-preparation against EHEC for cattle

This paper optimizes and scales up EIT antigen production in E. coli fermentations for a cattle vaccine against EHEC, achieving 350 doses per liter.

Enterococcus faecalis Influences the Transcription and Metabolism of Pathogenic Escherichia coli when Grown in Co-Culture

This study examines how Enterococcus faecalis affects the transcriptome and metabolism of Escherichia coli in co-culture, revealing strain-specific responses and nutrient cross-feeding.

The lac operon in uropathogenic Escherichia coli enhances intracellular growth by enabling host glycan utilization

This study shows that the lac operon in uropathogenic E. coli enables host glycan utilization, enhancing intracellular growth and infection dissemination.

Phage-encoded sRNA counteracts xenogenic silencing in pathogenic E. coli

The paper identifies a prophage-encoded sRNA, HnrS, that counteracts xenogenic silencing by repressing Hha, enabling virulence gene expression in E. coli.

E. coli associated with Crohn's disease exhibit distinct strategies to colonize macrophages

The paper shows that AIEC E. coli strains use different strategies to colonize macrophages and form IBCs, with variations in detoxification, biofilm formation, and macrophage responses.

The pcnB gene sustains Shigella flexneri virulence

The paper shows that the pcnB gene in Shigella flexneri promotes virulence by sustaining optimal levels of the virulence plasmid pINV, enhancing colonization and infection.

Co-evolution between colibactin production and resistance is linked to clonal expansions in Escherichia coli

The paper investigates the evolutionary dynamics of colibactin production and resistance in E. coli, showing clonal expansions linked to co-evolution between production and immunity.

LPS O-antigen polysaccharide length impacts outer membrane permeability of enteric gram-negative bacteria

This study shows that longer O-antigen polysaccharides on LPS in E. coli and Shigella flexneri weaken the outer membrane barrier, increasing antibiotic susceptibility.

Host range and zoonotic potential linked to P-like fimbrial adhesin specificity in avian pathogenic Escherichia coli

This study investigates the role of P-like fimbriae in avian pathogenic Escherichia coli, showing their species- and tissue-specific adherence and potential zoonotic risk.

Basic reproduction number for pandemic Escherichia coli clones varies markedly and can be comparable to pandemic influenza viruses

The paper estimates the basic reproduction number (R0) for pandemic E. coli clones and finds that ST131-A has an R0 comparable to pandemic influenza viruses.

Genome-scale CRISPRi profiling reveals metabolic vulnerabilities of uropathogenic Escherichia coli in human urine

This study uses CRISPRi to identify metabolic vulnerabilities of UPEC in urine, highlighting ALS II as a druggable target.

Cooperative Binding of Cytosolic Type III Secretion System Proteins to the Injectisome Revealed by Live-Cell Single-Molecule Localization Microscopy

This study uses live-cell single-molecule microscopy to show that cytosolic T3SS proteins bind to injectisomes with dynamics influenced by effector presence and secretion state.

Population structure, antimicrobial resistance, and virulence factors of diabetic foot-associated E. coli

This study analyzes the genomic diversity, antimicrobial resistance, and virulence factors of E. coli isolates from diabetic foot infections, revealing high multidrug resistance and virulence potential.

Bacterial warfare is associated with virulence and antimicrobial resistance

This study links bacterial warfare via bacteriocins to virulence and antimicrobial resistance in E. coli, showing their association with pathogenic strains and horizontal gene transfer.

Virus-like particle capture reveals coordination of actin remodeling during Shigella flexneri entry by host proteins

The paper identifies 14-3-3ζ and CAP2 as host proteins required for actin remodeling during Shigella flexneri entry, showing their coordinated role in membrane ruffle formation.