Escherichia coli research studies

Molecular Insights into CLD Domain Dynamics and Toxin Recruitment of the HlyA E. coli T1SS

The paper presents the first complete structural model of the HlyA T1SS using cryo-EM and SAXS, revealing CLD domain dynamics and toxin binding mechanisms.

Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei

This study identifies enhanced virulence and stress tolerance as factors contributing to the epidemiological success of Shigella sonnei, using zebrafish models and genomic analysis.

citrOgen: a synthesis-free polysaccharide and protein antigen-presentation to antibody-induction platform

The paper introduces a platform using Citrobacter rodentium to generate antibodies against bacterial polysaccharides and proteins without requiring purified or synthetic antigens.

Isolation, Identification and Determination of Antibiogram Characteristics of Escherichia Coli from Calves in West Shewa Zone, Oromia, Ethiopia.

This study isolates and identifies Escherichia coli from calves in Ethiopia and determines its antimicrobial susceptibility patterns, finding higher prevalence in diarrheic calves and multidrug resistance.

Improved in silico and in vitro methods for E. coli LPS outer core typing

This paper introduces nine oligonucleotides and a Python tool for in silico and in vitro typing of E. coli LPS outer core structures, revealing hybrid types and associations with pathogenicity and phylogeny.

Genetic control of Group 3 (K96) capsule synthesis and complement resistance in an extraintestinal pathogenic Escherichia coli ST69 strain

This study identifies genetic factors controlling Group 3 K96 capsule synthesis in ExPEC strain M12, showing its role in complement resistance and revealing phase-variable control and OxyR-dependent regulation.

Enteropathogenic Escherichia coli manipulates the host exocyst complex to enhance pedestal formation

This paper shows Enteropathogenic E. coli uses the exocyst complex to enhance pedestal formation alongside actin polymerization.

Use of Genome Scale Metabolic Reconstructions of Avian Pathogenic Escherichia coli (APEC) phylogroups for the identification of lineage-specific metabolic pathways

The paper constructs genome-scale metabolic models for APEC phylogroups B2, C, and G, identifying a lineage-specific metabolic pathway in phylogroup C that distinguishes it from other groups.

Evidence for Neolithic acquisition of the high pathogenic island by Escherichia coli followed by recent selection

The paper traces the evolutionary origin and recent selection of the High Pathogenicity Island in Escherichia coli, linking its acquisition to Neolithic human-animal interactions and modern public health implications.

Simple Sequence Repeats Mediate Phase Variation of the Mucoid Phenotype in Hypervirulent Klebsiella pneumoniae

This study identifies simple sequence repeats in rmpA and rmpD/rmpC genes that mediate phase variation of the mucoid phenotype in hypervirulent Klebsiella pneumoniae, enabling adaptation to different infection niches.

Mechanistic insight into the regulation of virulence factor type 1 pili in pathogenic E. coli by rhomboid protease GlpG

This study shows GlpG protease is essential for type 1 pili assembly in E. coli, affecting biofilm formation and bacterial fitness.

Macrophages orchestrate elimination of Shigella from the intestinal epithelial cell niche via TLR-induced IL-12 and IFN-γ

This study identifies macrophages, not neutrophils, as key in controlling Shigella through TLR-induced IL-12 and IFN-γ in a mouse model of shigellosis.

Evaluation of Klebsiella pneumoniae antigen MrkA reveals expression-limited therapeutic potential

The paper evaluates MrkA expression variability in Klebsiella pneumoniae and shows that anti-MrkA mAb efficacy depends on antigen abundance.

The growth and pathogenesis of Citrobacter rodentium is compromised by disrupted mucin sugar pathways that accumulate N-acetylglucosamine 6-phosphate

This paper shows that disrupting mucin sugar pathways in Citrobacter rodentium leads to accumulation of N-acetylglucosamine 6-phosphate, impairing bacterial growth and pathogenesis in mice.

High-risk clonal groups of Avian Pathogenic Escherichia coli (APEC) demonstrate heterogeneous phenotypic characteristics in vitro and in vivo

This study compares phenotypic differences among high-risk APEC clonal groups in vitro and in vivo, finding variation in invasion, survival, and virulence.

Invasion and intracellular proliferation of non-pathogenic E. coli in model infection systems

This study shows that a non-pathogenic E. coli strain can exhibit pathogenic traits under infection conditions using live-cell fluorescence microscopy.

A translocation-competent pore is required for Shigella flexneri to escape from the double membrane vacuole during intercellular spread

This study shows that IpaC forms a translocation-competent pore essential for Shigella flexneri to escape double membrane vacuoles during intercellular spread.

The natural diversity of E. coli transporter-dependent capsules

The paper maps E. coli capsule serotypes using genomic data, identifies novel K-types, and develops an in silico serotyping tool applicable to diverse E. coli genomes.

Enteropathogenic E. coli-mediated Fast and Coordinated Ca2+ responses regulate NF-κB activation

This study shows EPEC induces fast, coordinated Ca2+ responses via eATP, leading to reduced NF-κB activation through dampened O-linked β-N-acetylglucosamine modification.

Multiplexed PCR to measure in situ growth rates of uropathogenic E. coli during experimental urinary tract infection.

This paper introduces a PCR-based method to measure bacterial growth rates during infection, showing it correlates with WGS-based methods and reveals UPEC growth dynamics in a mouse UTI model.

Convergent evolution of distinct D-ribulose utilisation pathways in attaching and effacing pathogens

The paper identifies convergent evolution of D-ribulose utilisation pathways in attaching and effacing pathogens, showing how EHEC and Citrobacter rodentium exploit this nutrient for gut colonization.

From Colonisation to Invasion: Genomic and Phenotypic Comparison of Faecal and Bloodstream Isolates from the same patients

This study compares genomic and phenotypic differences between faecal and bloodstream isolates from the same patients to understand pathogenesis of Gram-negative bloodstream infections.

Lymphostatin: Structure of a large multi-functional virulence factor

This paper determines the structure of lymphostatin, a multifunctional virulence factor in E. coli, revealing its domains and mechanism of action.

Enterococcal cell wall remodelling underpins pathogenesis via the release of the Enteroccocal Polysaccharide Antigen (EPA)

This paper identifies AtlE, an N-acetylmuramidase essential for virulence in E. faecalis, showing its role in cell wall remodelling and immune evasion via the release of EPA-containing fragments.

Large-scale genomic analysis reveals the distribution and diversity of Type VI Secretion Systems in Escherichia coli

This study analyzes T6SS distribution in 131,610 E. coli genomes, revealing subclass prevalence, associations with pathotypes, and relationships with virulence and resistance genes.

LDL receptor-mediated endocytosis of Escherichia coli α-hemolysin mediates renal epithelial toxicity

This study identifies LDLR-mediated clathrin-dependent endocytosis as the mechanism by which HlyA from UPEC causes renal epithelial toxicity.

Growth dynamics and protein-expression of Escherichia coli serotypes O26:H11, O111:H8 and O145:NM in the bovine rumen.

This study compares the growth and protein expression of non-O157 STEC serotypes and non-STEC E. coli in bovine rumen environments under different dietary conditions.

Determinants of the Divergent Salmonella and Shigella Epithelial Colonization Strategies Resolved in Human Enteroids and Colonoids

This study compares Salmonella and Shigella colonization strategies in human enteroids and colonoids, identifying distinct virulence factor modules that drive divergent infection kinetics and epithelial colonization patterns.

Cryo-EM structures of engineered Shiga toxin-based immunogens capable of eliciting neutralizing antibodies with therapeutic potential against Hemolytic Uremic Syndrome

This paper presents cryo-EM structures of engineered Shiga toxin-based immunogens that stabilize pentamers and show interaction with neutralizing antibodies, supporting their use in a vaccine for STEC-HUS.

Ecotype-specific genomic features within the Escherichia cryptic clade IV

The paper characterizes a new Escherichia cryptic clade IV strain from an urban stream, revealing genomic features linked to environmental adaptation and ecological origin.