Reproductive Biology and Fertility

Reconstitution of chromatin reorganization during mammalian oocyte development

This study identifies RNA polymerase II degradation as the driver of chromatin reorganization from non-surrounded nucleolus to surrounded nucleolus in mammalian oocytes.

Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes

This study identifies impaired maternal mRNA deadenylation as a molecular defect in in vitro matured mouse and human oocytes, leading to abnormal gene expression.

Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes

The paper uses single-cell proteomics to show that advanced maternal age oocytes have reduced proteostasis and meiosis proteins.

Interaction between gene expression and morphokinetic parameters in undisturbed human embryo culture

This study compares gene expression in human embryos cultured in time-lapse systems versus conventional incubators and identifies molecular mechanisms linking morphokinetic parameters to embryo development.

SMAD4 promotes somatic-germline contact during murine oocyte growth

The paper shows that SMAD4 deletion in granulosa cells reduces transzonal projections (TZPs) contacting oocytes, suggesting TGFβ signaling stabilizes soma-germline communication.

Distinct waves of ovarian follicles contribute to mouse oocyte production

The paper uses scRNA-seq and lineage tracing to study mouse ovarian follicle waves, finding that wave 1 follicles undergo atresia and contribute to steroidogenesis, while wave 1.5 follicles generate most early oocytes.

Theca cell mechanics and tissue pressure regulate mammalian ovarian folliculogenesis

This study shows that theca cells regulate ovarian follicle growth through mechanical signaling and tissue pressure, affecting granulosa cell behavior and oocyte development.

Autologous ovarian tissue transplantation and longevity improvement (experimental mice model).

The study shows that step-by-step autologous ovarian transplantation improves longevity in mice more effectively than hormonal replacement therapy.

Karyopherin α2 is a maternal effect gene required for early embryonic development and female fertility in mice

This study shows that KPNA2 deficiency in mice causes embryonic arrest at the 2-cell stage and disrupts genome activation and nuclear translocation of Nucleoplasmin 2.

FOXL2 interaction with different binding partners regulates the dynamics of ovarian development

The paper investigates FOXL2's role in ovarian development through multi-omics analysis, identifying its binding partners and regulatory targets, and shows that USP7 deletion causes infertility.

Identification of novel genes in cattle (Bos taurus) and biological insights into their function in embryo development

The paper identifies 3,033 potential new genes in cattle oocytes and embryos using transcriptome sequencing and validates one gene's role in blastocyst development through CRISPR-Cas9.

In Vitro Culture Alters Cell Lineage Composition and Cellular Metabolism of Bovine Blastocyst

The paper uses single-cell transcriptomics to compare bovine blastocysts cultured in vivo versus in vitro under different conditions, revealing how culture affects cell lineage and metabolism.

Dose-response functional and transcriptomic effects of follicle-stimulating hormone on ex vivo mouse folliculogenesis

This study investigates the dose-response effects of FSH on mouse folliculogenesis using single-cell RNA sequencing, revealing gene expression patterns and potential molecular mechanisms of FSH-induced ovarian disorders.

Mechanism Study on the Effect of Kidney Tonifying and Liver Soothing Methods on Mouse Oocyte Quality by Regulating OSFs and Smads Pathways

This study investigates how kidney tonifying and liver soothing methods affect mouse oocyte quality by regulating BMP-6 and Smads pathways through protein and mRNA expression analysis.

Molecular signatures associated with successful implantation of the human blastocyst

The paper identifies molecular signatures in human blastocysts associated with successful implantation by analyzing gene expression and cell-cell interactions in embryos with different morphological grades.

A Spatiotemporal Molecular Atlas of the Ovulating Mouse Ovary

This study maps the spatiotemporal molecular changes during mouse ovary follicle maturation and ovulation, identifying key cell-type-specific programs and interactions.

Multiple intersecting pathways are involved in the phosphorylation of CPEB1 to activate translation during mouse oocyte meiosis

This study identifies CDK1/MAPK and AURKA/PLK1 pathways as converging on CPEB1 phosphorylation during mouse oocyte meiosis, showing CDK1 is critical for translation activation.

Oocyte and cumulus cell cooperativity and metabolic plasticity under the direction of oocyte paracrine factors

This study shows how oocyte-secreted factors alter proteomic and metabolic profiles of oocytes and cumulus cells during maturation, enhancing their cooperativity for fertilization.

Cell specification and functional interactions in the pig blastocyst inferred from single cell transcriptomics

The paper uses single-cell RNA sequencing and proteomics to analyze pig blastocyst development, identifying new cell subpopulations and regulatory networks.

The oocyte microenvironment is altered in adolescents compared to oocyte donors.

This study compares the molecular signatures of cumulus cells and follicular fluid between adolescents and oocyte donors, finding significant differences in gene expression and cytokine profiles.

First cleavage is a manifestation of the geometry of the unfertilized oocyte: implications for monozygotic twinning in mice.

The paper shows that the orientation of the first cleavage in mouse oocytes follows the shorter diameter of the unfertilized oocyte, leading to segregation of animal and vegetal hemispheres and explaining differences in monozygotic twins.

Age-associated aberrations of cumulus-oocyte interaction and microfilamentous structure in the zona pellucida decline female fertility

This study shows that age-related structural changes in the zona pellucida reduce fertilization rates in mice by impairing cumulus-oocyte interactions and sperm binding.

GametesOmics: A Comprehensive Multi-Omics Database for Exploring the Gametogenesis in Humans and Mice

GametesOmics integrates multi-omics data from human and mouse gametogenesis to provide tools for analyzing gene expression, DNA methylation, and chromatin accessibility.

Improved mRNA electroporation into immature oocytes to examine protein dynamics during development

This study develops a low-invasive electroporation method for mRNA delivery into mouse oocytes, achieving high survival rates and enabling chromosomal dynamics visualization.

Artificial environment impact: O2 concentration changes between IVM and IVF alter embryo production, metabolism, and epigenetic marks

This study examines how varying oxygen concentrations during in vitro maturation and fertilization affect bovine embryo production, metabolism, and epigenetic marks.

OOCYTE AND EMBRYO CULTURE UNDER OIL PROFOUNDLY ALTERS EFFECTIVE CONCENTRATIONS OF SMALL MOLECULE INHIBITORS

The paper shows that oil-covered culture systems reduce the effectiveness of small molecule inhibitors in oocyte and embryo experiments, affecting EC50 values and suggesting potential issues with reproducibility.

Transcriptome analysis of the mouse fetal and adult rete ovarii and surrounding tissues

This study analyzes bulk, single-cell, and single nucleus transcriptomes of fetal and adult mouse rete ovarii to identify gene expression signatures and differences across RO regions.

Superovulation and aging perturb oocyte-granulosa cell communication

This study investigates how superovulation and aging affect oocyte-granulosa cell communication in mice, finding that both disrupt similar gene sets and impair oocyte quality.

Intracellular fraction of zona pellucida protein 3 is required for the oocyte to embryo transition in mice

This study identifies a novel intracellular function of zona pellucida proteins in mice, showing they are critical for embryonic cell survival during the oocyte-to-embryo transition.

Impact of repeated cryopreservation on embryo health and implantation potential

This study evaluates the impact of repeated cryopreservation on embryo health and implantation potential in mice, finding reduced survival and inner cell mass cells after multiple freeze-thaw cycles.