Reproductive Biology and Fertility

Fertility is compromised after oocyte-specific deletion of Katanin A-subunit, Katna1, but not Katnal1

This study shows that deleting Katna1 in mouse oocytes reduces fertility, while Katnal1 deletion has no effect, with defects in MII spindle morphology and fertilization rates.

CHEK2 SIGNALING IS THE KEY REGULATOR OF OOCYTE SURVIVAL AFTER CHEMOTHERAPY

This study shows that inhibiting CHEK2 protects primordial oocytes from chemotherapy-induced damage, suggesting it could be used to preserve ovarian function in cancer patients.

C-type natriuretic peptide improves maternally aged oocytes quality by inhibiting excessive PINK1/Parkin-mediated mitophagy

This study shows that C-type natriuretic peptide improves oocyte quality in aged mice by reducing oxidative damage and suppressing excessive mitophagy through the cAMP/PKA pathway.

Comprehensive comparison of female germ cell development in vitro and in vivo identifies epigenetic gene regulation crucial for oocyte development and embryonic competence

Differential effects of follicle-stimulating hormone glycoforms on the transcriptome profile of cultured rat granulosa cells as disclosed by RNA-seq

This study uses RNA-seq to analyze how different FSH glycoforms affect gene expression, biological processes, and pathways in rat granulosa cells over time.

Luteinizing hormone stimulates ingression of granulosa cells within the mouse preovulatory follicle.

The paper shows LH induces granulosa cell ingression in the mural granulosa layer, contributing to structural changes during ovulation.

Human induced pluripotent stem cell-derived ovarian support cell co-culture improves oocyte maturation in vitro after abbreviated gonadotropin stimulation

This study shows that co-culturing human oocytes with ovarian support cells improves in vitro maturation rates and blastocyst formation compared to standard IVM methods.

Mapping the anatomical and transcriptional landscape of early human fetal ovary development

This study maps the anatomical and transcriptional changes in human fetal ovaries during development using single-nuclei RNA sequencing and other techniques, identifying novel pathways and regulators.

CD38 regulates ovarian function and fecundity via NAD+ metabolism

This study shows CD38 loss increases ovarian NAD+ levels and fecundity in young mice but not in older mice.

The longitudinal single-cell panorama of cynomolgus monkey ovary throughout lifespan revealed a conserved ovarian clock between primates

The paper constructs a longitudinal single-cell atlas of cynomolgus monkey ovaries across 22 years, identifies a novel oocyte type, and develops a transcriptomic ovarian clock applicable to humans.

Akr1b7 functions as a master regulator in ovarian aging

This study identifies Akr1b7 as a key regulator in ovarian aging, showing its role in oocyte maturation and estrous cycle regulation in mice.

Effects of lactate, super-GDF9 and low oxygen tension during biphasic in vitro maturation on the bioenergetic profiles of mouse cumulus-oocyte-complex

This study evaluates the bioenergetic profiles of mouse cumulus-oocyte-complexes during biphasic IVM, showing that low oxygen tension improves metabolic similarity to in vivo maturation.

Using unlabeled information of embryo siblings from the same cohort cycle to enhance in vitro fertilization implantation prediction

The paper uses sibling embryo data from the same IVF cohort to improve machine learning prediction of embryo implantation outcomes.

Zn2+ is Essential for Ca2+ Oscillations in Mouse Eggs

This study shows that Zn2+ is essential for Ca2+ oscillations in mouse eggs, with both insufficient and excessive Zn2+ disrupting these responses.

Deep learning pipeline reveals key moments in human embryonic development predictive of live birth in IVF

The paper uses deep learning to identify key developmental stages in human embryos that predict viability, improving IVF embryo selection.

Assessing the influence of distinct IVF culture media on human pre-implantation development using single-embryo transcriptomics

This study uses single-embryo transcriptomics to assess how different IVF culture media affect gene expression in human preimplantation embryos, showing medium-specific effects that diminish by the blastocyst stage.

Maternal regulation of the vertebrate oocyte-to-egg transition

This paper identifies maternal-effect mutants in zebrafish that reveal molecular mechanisms controlling the oocyte-to-egg transition, focusing on yolk globule formation and protein processing.

Biallelic Variants in MAD2L1BP (p31comet) Cause Female Infertility Characterized by Oocyte Maturation Arrest

This study identifies biallelic MAD2L1BP variants causing oocyte maturation arrest in female infertility and shows functional rescue with full-length cRNAs.

MYC is essential for induction of major ZGA and subsequent preimplantation development

This study shows that Myc is essential for zygotic genome activation (ZGA) and preimplantation development in mouse embryos.

Delay of human early development via in vitro diapause

This study shows that human blastoids and pluripotent stem cells can enter a dormant state via mTOR inhibition, with distinct temporal dynamics compared to mice.

REGENERATIVE GENE THERAPY IN THE HYPOTHALAMUS PROLONGS FERTILITY IN FEMALE RATS

This study shows that long-term hypothalamic gene therapy with Yamanaka factors extends fertility in middle-aged female rats by preserving ovarian function and cyclicity.

Branched germline cysts and female-specific cyst fragmentation facilitate oocyte determination in mice

The paper identifies branched germline cyst structures in mice and shows they facilitate oocyte determination through directional cytoplasmic transport and cyst fragmentation.

LSM14B is essential for mitochondrial clustering in the oocyte meiosis

This study shows LSM14B is essential for MARDO assembly in mouse oocytes, leading to meiotic maturation failure and infertility.

Retrospective model utilizing biopsies, granulosa cells, and polar body to predict oocyte competence in bovine

This paper predicts oocyte competence in bovine using RNA-seq and WGBS from biopsies, cumulus cells, granulosa cells, and polar bodies, identifying transcriptional and epigenetic differences between cleaved but arrested and blastocyst-stage embryos.

Improved rescue of immature oocytes obtained from conventional gonadotropin stimulation cycles via human induced pluripotent stem cell-derived ovarian support cell co-culture

This study shows that co-culturing immature human oocytes with hiPSC-derived ovarian support cells improves in vitro maturation rates and transcriptomic similarity to in vivo matured oocytes.

Macrophage activation drives ovarian failure and masculization

The paper identifies a macrophage-germline-somatic gonadal cell axis driven by BMP15 mutation that contributes to ovarian failure and masculization in zebrafish.

Genomic insights into southern white rhinoceros ( Ceratotherium simum simum ) reproduction: revealing granulosa cell gene expression

This study analyzes gene expression in granulosa cells of southern white rhinoceros to inform assisted reproductive technologies.

Multi-omics analysis demonstrates a critical role for GLP methyltransferase in transcriptional repression during oogenesis

The paper investigates the role of GLP methyltransferase in oocyte development using multi-omics analysis and mouse models, finding that GLP contributes to transcriptional repression and is critical for oogenesis.

Lack of accelerated ovarian aging in a follicle-stimulating hormone receptor haploinsufficiency model

This study shows that KOMP Fshr heterozygous knockout mice do not exhibit accelerated ovarian aging phenotypes previously reported in another Fshr haploinsufficiency model.

More accurate analysis of maternal effect genes by siRNA electroporation into mouse oocytes

This study introduces an electroporation method to suppress maternal effect genes in mouse oocytes without removing cumulus cells, showing efficient maternal mRNA suppression and revealing H3.3's role in post-fertilization development.