Fungal and yeast genetics research

Telomere to telomere sequence of model Aspergillus fumigatus genomes

The paper presents full-length telomere-to-telomere genome assemblies for two Aspergillus fumigatus strains using long-read sequencing technologies.

Functional variability in adhesion and flocculation of yeast megasatellite genes

The paper investigates the functional roles of megasatellites in yeast adhesion and flocculation by deleting genes and evolving strains.

Deletion of can1/cat1 genes and expression of a dominant any1 mutation establish an effective canavanine selection in fission yeast

This study identifies a dominant any1 mutation that confers strong canavanine resistance in fission yeast, enabling its use as a genetic selection marker.

Transcriptome and proteome profiling reveals complex adaptations of Candida parapsilosis cells assimilating hydroxyaromatic carbon sources

This study profiles transcriptome and proteome of Candida parapsilosis assimilating hydroxyaromatic carbon sources, revealing pathway regulation and metabolic connections.

Growth-Rate Dependent And Nutrient-Specific Gene Expression Resource Allocation In Fission Yeast

This study investigates how growth rate and nutrient-specific factors influence gene expression in fission yeast, revealing coordinated and condition-specific regulation of metabolic enzymes.

Network Analysis Reveals Different Cellulose Degradation Strategies across Trichoderma harzianum Strains Associated with XYR1 and CRE1

The paper compares cellulose degradation strategies in two Trichoderma harzianum strains using network analysis, revealing strain-specific regulatory differences involving XYR1 and CRE1.

Cre/lox-mediated chromosomal integration of biosynthetic gene clusters for heterologous expression in Aspergillus nidulans

The paper develops a system for targeted chromosomal integration of biosynthetic gene clusters in Aspergillus nidulans using Cre/lox-mediated recombination, demonstrating efficient integration and expression of a 21 kb DNA fragment.

Epistatic genetic interactions govern morphogenesis during sexual reproduction and infection in a global human fungal pathogen

This study identifies genetic interactions governing morphogenesis in Cryptococcus fungi using QTL analysis, revealing epistatic relationships between genes involved in signaling pathways.

A transcription factor and a phosphatase regulate temperature-dependent morphogenesis in a fungal plant pathogen

The paper identifies two genes regulating temperature-dependent morphogenesis in Z. tritici, showing that intracellular osmotic stress drives these transitions.

A cellular and molecular atlas reveals the basis of chytrid development.

This study creates a cellular and molecular atlas of chytrid development using Rhizoclosmatium globosum, revealing specialized biological processes and subcellular structures.

Multi-omics analysis of multiple glucose-sensing receptor systems in yeast

This study compares two glucose receptor systems in yeast, showing they regulate distinct metabolic processes and signaling pathways.

On the illusion of auxotrophy: met15Δ yeast cells can grow on inorganic sulfur thanks to the previously uncharacterized homocysteine synthase Yll058w

The paper shows that yeast cells lacking Met15 can assimilate inorganic sulfur via Yll058w, but require exogenous methionine or hydrogen sulfide chelators to grow, challenging the traditional view of MET15 as essential for sulfur assimilation.

A natural fungal gene drive enacts killing through targeting DNA

The paper identifies Spok1, a fungal spore killer protein, as a DNA-targeting agent that causes killing in fungi and functions across bacteria and yeast.

Meiosis in the human pathogen Aspergillus fumigatus has the highest known number of crossovers

The paper shows that meiosis in Aspergillus fumigatus has the highest known crossover rate, producing ~29 crossovers per chromosome, and can generate azole-resistant haplotypes.

Chromatin profiling reveals genome stability heterogeneity in clinical isolates of the human pathogen Aspergillus fumigatus

The paper compares chromatin activities in two A. fumigatus isolates, finding higher genome instability in Af293 and identifying a variant with chromosomal alterations linked to increased virulence.

Heat Stress Regulates the Expression of TPK1 Gene at Transcriptional and Post-Transcriptional Levels in Saccharomyces cerevisiae

The paper shows heat stress increases TPK1 mRNA levels and stabilizes it in cytoplasmic foci, impairing translation, while the CWI pathway via Wsc3 regulates this response in Saccharomyces cerevisiae.

Fission stories: Using PomBase to understand Schizosaccharomyces pombe biology

The paper describes PomBase, a database for fission yeast, and highlights its features and contributions to the model organism database community.

New Data and Collaborations at the Saccharomyces Genome Database: Updated reference genome, alleles, and the Alliance of Genome Resources

The paper describes recent updates to the Saccharomyces Genome Database, including a new reference genome, allele curation system, and resources for studying human disease using yeast.

Host defense mechanisms induce genome instability in an opportunistic fungal pathogen

This study shows that host-produced reactive oxygen species and antimicrobial peptides induce genome instability in Candida albicans, leading to genetic changes that may enhance its adaptability.

Combined Pan-, Population-, and Phylo-Genomic Analysis of Aspergillus fumigatus Reveals Population Structure and Lineage-Specific Diversity

This study combines pan-, population-, and phylo-genomic analyses of Aspergillus fumigatus to reveal population structure, lineage-specific diversity, and ecological drivers of virulence.

Genomic characterization of a pathogenic isolate of Saccharomyces cerevisiae reveals an extensive and dynamic landscape of structural variation

The paper presents a comprehensive genomic structural analysis of a pathogenic S. cerevisiae isolate using long-read sequencing, revealing extensive structural variation and its implications for genomic stability and development.

Multiple causal DNA variants in a single gene affect gene expression in trans

The paper identifies multiple causal DNA variants within a single gene that affect gene expression in trans in yeast, showing complexity in trans-acting variation.

Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit

The paper develops SECRiFY, a method to assess secretability of protein fragments in yeast, revealing intrinsic disorder and flexibility as key factors.

Sex without crossing over in the yeast Saccharomycodes ludwigii

The paper shows that Saccharomycodes ludwigii undergoes meiosis without recombination, relying on frequent fertilization within tetrads instead.

KTD1 is a yeast defense factor against K28 killer toxin

The paper identifies KTD1 as a novel defense factor in Saccharomyces cerevisiae that confers resistance to the K28 killer toxin.

Genetic Interaction between RLM1 and F-box Motif Encoding gene SAF1 Contributes to Stress Response in Saccharomyces cerevisiae

The paper identifies a genetic interaction between SAF1 and RLM1 in Saccharomyces cerevisiae that influences stress response, showing differential tolerance to various stressors.

A novel and effective Cre/lox-based genetic tool for repeated, targeted and markerless gene integration

This paper develops a Cre/lox-based genetic tool for markerless, repeated gene integration in Yarrowia lipolytica with high efficiency.

Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase

The paper describes engineered yeast shuttle vectors that allow efficient modification of replication loci using Cre recombinase and restriction enzymes.

S. pombe wtf genes use dual transcriptional regulation and selective protein exclusion from spores to cause meiotic drive

The paper investigates how wtf4 meiotic drivers in S. pombe use dual transcriptional regulation and selective protein exclusion to ensure their transmission through gametes.

A yeast with muscle does not run faster: full humanization of the glycolytic pathway in Saccharomyces cerevisiae

This study successfully humanizes the entire glycolytic pathway in yeast, revealing conserved functions and context-dependent responses.