Advanced Fluorescence Microscopy Techniques

Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging

This paper introduces Rho-tag and SiR-tag, fast, reversible, and fluorogenic protein tags for live-cell imaging, compatible with super-resolution techniques and demonstrated in zebrafish larvae.

Cortex-Wide, Cellular-Resolution Volumetric Imaging with a Modular Two-Photon Imaging Platform

The paper introduces Meso2P, a two-photon imaging platform that enables high-resolution, large-scale volumetric imaging of the cortex with modular upgrades for multi-plane and optogenetic stimulation.

Array Detection Enables Large Localization Range for Simple and Robust MINFLUX

This paper introduces ISM-FLUX, a MINFLUX implementation using a SPAD array detector, achieving larger localization range and simpler architecture.

SNAP-tag2: faster and brighter protein labeling

This paper introduces SNAP-tag2, an improved version of SNAP-tag with faster labeling kinetics and higher fluorescence brightness for better live-cell imaging.

Model-free photon analysis of diffusion-based single-molecule FRET experiments

The paper introduces a model-free method to analyze diffusion-based single-molecule FRET experiments, showing it accurately captures protein dynamics without artifacts from photon trajectory length or diffusion.

Multiparametric Correlative Topographical and Volumetric Fluorescence Microscopy

This paper introduces a combined SICM-OPM system for simultaneous non-contact topographical and volumetric fluorescence imaging in live cells, demonstrating its use in studying cellular remodeling and molecular delivery.

Label-free visualization of ciliary rootlets in mouse brain

This paper introduces a label-free imaging method to visualize ciliary rootlets in mouse brain tissue, revealing their organized distribution and formation timing relative to primary cilia.

Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues

The paper introduces ExA-SPIM, a microscope that enables high-resolution imaging of large tissue samples without sectioning, demonstrating its use on mouse brains and human white matter.

Optimization of Tissue Clearing Methods and Imaging Conditions for 3D Visualization of the Vasculature of the Adult Murine Knee

This study optimizes tissue clearing and imaging methods to achieve high-resolution 3D visualization of the vasculature in adult murine knee tissues.

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy

This paper designs de novo Rhodamine Binder (Rhobin) tags with high brightness, stability, and applicability in live-cell imaging and extreme conditions.

Two-Point Calibration Protocol for the FRET Indicator Pyronic in Neurons

This paper presents a two-point calibration protocol for the FRET indicator Pyronic to measure cytosolic pyruvate concentrations in neurons at single-cell resolution.

A General Strategy to Endow Dyes with Genic Fluorescence for Wash-Free Imaging of Proteins in Living Cells

This paper introduces a method to enhance fluorescent dye imaging in living cells by using FRET pairs to reduce background noise and improve signal-to-noise ratio.

Toward Crosstalk-free All-optical Interrogation of Neural Circuits

This paper introduces an active pixel power control method to reduce crosstalk in two-photon optogenetics and calcium imaging, improving the accuracy of neural circuit analysis in zebrafish.

A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification

This paper introduces a 3D immunolabeling method for high-speed and high-sensitive detection of targets in 3D tissues, demonstrating its application in visualizing neuronal and glial cells in mouse brain tissues.

Cell barcoding with tandem fluorescent proteins enables high-throughput signaling dynamics analysis

This paper introduces sctFP barcodes for high-throughput signaling dynamics analysis by enabling multiplexed biosensor tracking through reliable fluorescence signal differentiation.

Quantifying Uncertainty in Phasor-Based Time-Domain Fluorescence Lifetime Imaging Microscopy

This study develops a theoretical uncertainty model for phasor-based FLIM to quantify noise effects on lifetime estimation and fluorophore unmixing, validated with simulations and experiments.

Deep learning for fluorescence lifetime predictions enables high-throughput in vivo imaging

FLIMngo, a deep learning model, enables fast and accurate fluorescence lifetime predictions from low-photon FLIM data, improving throughput and reducing phototoxicity for in vivo applications.

Fast Photostable Expansion Microscopy Using QDots andDeconvolution

This paper introduces a method combining quantum dots, widefield imaging, and deconvolution to improve the speed and signal-to-noise ratio of expansion microscopy.

Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice

This study develops sciDISCO, an optimized tissue clearing protocol for spinal cord imaging in mice, enabling 3D analysis of intact and injured tissue.

Tunable fluorogenic DNA probes drive fast and high-resolution single-molecule fluorescence imaging

The paper introduces tunable fluorogenic DNA probes that enable high-concentration single-molecule fluorescence imaging, overcoming the high concentration barrier and enabling faster and higher-resolution imaging.

MiniXL: An open-source, large field-of-view epifluorescence miniature microscope for mice capable of single-cell resolution and multi-brain region imaging

Electrokinetic detection of single-molecule phosphorylation

This study demonstrates electrokinetic detection of single-molecule phosphorylation, resolving phosphorylated sites and monitoring kinase cycles with single turnover resolution.

Oblique Thick Light-Sheet Microscopy with Spatiotemporal 3D Localization for RNA Imaging in Intact 50 μm-Thick Sections

This paper introduces a microscopy technique that enables 3D RNA imaging in thick brain sections by combining spatiotemporal localization with volumetric excitation, revealing detailed neuronal distributions and expression gradients.

Parallelization of single-molecule binding kinetic measurements via protein barcode sequencing

This paper introduces a method to parallelize single-molecule binding kinetic measurements using protein barcode sequencing, enabling rapid and multiplexed screening of protein libraries.

In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors

The paper introduces a far-red dopamine sensor, HaloDA1.0, enabling multiplex imaging of neurochemical networks in vivo with high sensitivity and versatility.

Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view

This paper introduces a model-based method to estimate field-dependent aberrations in single molecule localization microscopy without calibration, improving localization accuracy across large fields of view.

A Set of Novel Far-Red Fluorescent Proteins for Temporal Domain Multiplexing and Super-Resolution Imaging

The paper introduces novel far-red fluorescent proteins for temporal domain multiplexing and super-resolution imaging, enabling subcellular structure distinction without hardware modifications.

A parallelly distributed microscope and software system for scalable high-throughput multispectral 3D imaging

The paper introduces a parallelly distributed microscope and software system for high-throughput multispectral 3D imaging with high resolution and data throughput.

Bright and Photostable Voltage Sensors Derived from mBaoJin

This paper introduces two photostable voltage sensors, ElectraON and ElectraOFF, which enable longer in vivo recordings by improving photostability compared to existing GEVIs.

Focus-Tunable Two-Photon Fiberscope Enabling in vivo Imaging at Selected Depths

This paper introduces a two-photon fiberscope with tunable focus for in vivo imaging at selected depths, demonstrating its performance using phantoms and biological samples.