Advanced Fluorescence Microscopy Techniques

In vivo deep-brain microscopy at submicrometer resolution with refractive index-matched prism interfaces

This paper develops an implantable microprism interface for deep-brain imaging with submicrometer resolution and wide-field two-photon microscopy.

Prolonged Single Neuron Voltage Imaging in Behaving Mammals

The paper demonstrates prolonged single-neuron voltage imaging in behaving mice using ElectraOFF, revealing plasticity changes during neuromodulation.

Single Particle Tracking of Genetically Encoded Nanoparticles: Optimizing Expression for Cytoplasmic Diffusion Studies

This paper optimizes genetically encoded nanoparticle expression to improve single-particle tracking of cytoplasmic diffusion in mammalian cells, showing increased diffusivity and better tracking quality.

Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment

This paper introduces a DNA-PAINT system with optical photon reassignment on a spinning disk confocal to achieve high-resolution imaging in thick samples and large fields of view.

A photostable genetically encoded voltage indicator for imaging neural activities in tissue and live animals

The paper introduces Vega, a photostable GEVI with high sensitivity and fast response, enabling high-fidelity voltage imaging in tissue and live animals.

FLIMPA: A versatile software for Fluorescence Lifetime Imaging Microscopy Phasor Analysis

FLIMPA is an open-source software for FLIM phasor analysis that integrates data from multiple trials and provides tools for quantifying microtubule depolymerisation.

Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution

This paper demonstrates three-photon in vivo imaging in the mouse medial prefrontal cortex to achieve sub-cellular resolution of neuronal and glial activity at unprecedented depths.

Gradual labeling with fluorogenic probes for MINFLUX nanoscopy of densely packed structures

This paper introduces GLF-MINFLUX, a method for nanoscale imaging of densely packed structures, achieving improved localization precision and faster acquisition.

Imaging neuronal voltage beyond the scattering limit

This paper introduces a method to image neuronal voltage with super-resolution beyond tissue scattering limits, achieving high precision in dense neuronal populations.

Near-Zero Photon Bioimaging

This paper introduces near-zero photon imaging, a method that captures high-fidelity biological images at ultralow irradiance and high speed using AI.

Triple labeling resolves a GPCR intermediate state by 3-color single molecule FRET

This paper introduces a triple-labeling strategy for single molecule FRET to study conformational changes in GPCRs, revealing a previously unknown intermediate state.

DeepTRACE: Flexible Machine Learning for Analysis and Discovery in Single Molecule Tracks

DeepTRACE is a machine learning tool that analyzes single-molecule tracks in living cells, uncovering molecular processes and relationships not present in training data.

Multimodal Phasor Approach to study breast cancer cells invasion in 3D spheroid model

The paper uses a multimodal imaging approach to study breast cancer cell invasion in 3D spheroids, quantifying changes in metabolism, fatty acid storage, and collagen organization over time.

IntAct-U-ExM: Ultrastructure Expansion microscopy of actin networks via an internally-tagged actin

The paper develops IntAct-U-ExM to visualize actin networks with super-resolution, showing improved resolution of actin structures in yeast and mammalian cells.

A Spectral Image Scanning Microscope for Multi-Color High-Resolution Imaging

This paper introduces a spectral CSD-ISM system for high-resolution, multi-color imaging with improved speed and resolution.

PerfectlyAverage: a classical open-source software method to determine the optimal averaging parameters in laser scanning fluorescence microscopy

PerfectlyAverage determines optimal averaging parameters in laser scanning fluorescence microscopy by analyzing SNR, photobleaching, and PSD to reduce acquisition time.

Chip-Based 3D Interferometric Nanoscopy

This paper introduces SiLM, a chip-based 3D single-molecule localization microscopy method that achieves high axial precision with a single-objective lens, comparable to 4Pi methods.

Dark-based Optical Sectioning assists Background Removal in Fluorescence Microscopy

This paper introduces a method to remove defocus backgrounds in fluorescence microscopy using dark channels and dual frequency separation, significantly improving image quality.

Novel Fluorescent and Photoconvertible Fusions Reveal Dorsal Activator Dynamics

The paper introduces four new fluorescent protein fusions for Dorsal to study its dynamics in fruit fly embryos.

Identifying Intermolecular Interactions in Single-Molecule Localization Microscopy

This paper develops a probabilistic algorithm to accurately identify and quantify intermolecular interactions using single-molecule localization microscopy.

DNA-PAINT imaging with hydrogel imprinting and clearing

This study shows that polyacrylamide hydrogels support DNA-PAINT imaging by preserving nanoscale docking strand positioning and enhancing diffusion and accessibility.

CoSiDeX: A hyperspectral fluorescent protein resource for highly multiplexed imaging

The paper introduces CoSiDeX, a tool for selecting and decoding fluorescent protein palettes in multiplexed imaging experiments using hyperspectral data.

Model-free machine learning-based 3D single molecule localisation microscopy

This paper introduces a machine learning-based 3D SMLM method called easyZloc that works with standard microscopes and reduces computational demands.

Deep Volumetric Localization Super-Resolution Microscopy

This paper introduces VLM, a super-resolution microscopy method that combines hardware and algorithmic improvements to achieve high-fidelity 3D single-molecule imaging with 10 nm and 25 nm localization precision.

Bayesian Inference of Binding Kinetics from Fluorescence Time Series

This paper infers binding and unbinding rates from fluorescence time series while accounting for photobleaching using a two-stage Bayesian approach.

FRAP-in-SR: Fluorescence recovery in the Super-Resolution regime reveals subcompartments of 53BP1 foci

This study uses FRAP-SR to reveal subcompartments within 53BP1 foci, showing differential protein mobility and function in different foci types.

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

The paper compares monomeric StayGold variants using protein nanocages in living cells, finding mStayGold is 3-fold brighter and more photostable than EGFP and other variants.

A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging

This paper introduces a low-cost FPGA-based method to correct pile-up distortions in TCSPC-FLIM, enabling high-speed, accurate in vivo imaging with subcellular resolution.

Image-based 3D active sample stabilization on the nanometer scale for optical microscopy

This paper introduces an image-based 3D active stabilization method for super-resolution microscopy, achieving ~1 nm stability without requiring fiducial markers or complex hardware.

GEQO biosensors for absolute analyte quantification in single cells

The paper introduces GEQO biosensors for absolute analyte quantification in single cells without requiring specialized equipment, showing they account for buffering and retain time trace information.