Advanced Fluorescence Microscopy Techniques

Optical segmentation-based compressed readout of neuronal voltage dynamics

This paper introduces a novel optical segmentation method for compressed readout of neuronal voltage dynamics, achieving high-speed recording of subcellular activity with improved data efficiency.

Highly multiplexed spectral FLIM via physics informed data analysis

This paper introduces a Bayesian S-FLIM framework that learns spectra and lifetimes photon-by-photon, enabling efficient deconvolution of up to 9 fluorophore species with overlapping spectra.

Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera

This paper develops a high-throughput single-molecule FLIM technique using a time-gated single-photon camera, achieving precise lifetime measurements with spatial multiplexing of over 3000 molecules.

Design of Orthogonal Far-Red, Orange and Green Fluorophore-binding Proteins for Multiplex Imaging

The paper designs orthogonal fluorophore-binding proteins using deep learning for multiplex imaging, demonstrating high specificity and utility in labeling and monitoring subcellular processes.

Superresolution imaging of live samples by centroid reassignment microscopy

This paper introduces a superresolution microscopy technique called centroid reassignment microscopy (CRM) that enables fast, robust, and protocol-compatible imaging of live samples.

Simultaneous 2- and 3-photon multiplane imaging across cortical layers in freely moving mice

The paper introduces a head-mounted multiplane microscope that enables near-simultaneous imaging of neuronal activity from multiple cortical layers in freely moving mice.

Quantification of Cyclin-CDK dissociation constants in living cells using fluorescence cross-correlation spectroscopy with green and near-infrared fluorescent proteins.

This study measures cyclin-CDK dissociation constants in living cells using FCCS with optimized fluorescent proteins, revealing insights into cyclin-CDK binding redundancy.

Localisation of Apicomplexa motor Myosin A with axial nanometric precision using graphene energy transfer

This study localizes Myosin A in Toxoplasma gondii with 1-4 nm axial precision using graphene energy transfer, supporting the linear migration motor model.

Spinning disk confocal microscopy with a 25 Megapixel Camera

The paper presents a low-cost spinning disk confocal microscopy system using a 25 megapixel CMOS camera and custom hardware, validated with biological samples.

Determine Protein Interaction Affinity without Purification Reveals a Two-enzyme Co-recognition of SUMOylation Substrate using Quantitative FRET (qFRET) Technology

This paper develops a high-throughput qFRET method to determine protein interaction affinity without purification, revealing a two-enzyme co-recognition mechanism for SUMOylation substrates.

Assessing cellular metabolic dynamics with NAD(P)H fluorescence polarization imaging

This paper introduces FPRM, a fast, label-free imaging method to monitor cellular metabolism using NAD(P)H autofluorescence, and demonstrates its application in tracking metabolic changes under various perturbations.

Systematic Characterization of Optical Aberrations Reveals Cryo-FLM Localization Fidelity

This study systematically analyzes optical aberrations in cryo-FLM, showing they cause localization errors up to 300 nm axially and introduces models to reduce errors to under 10 nm.

The two key substitutions in the chromophore environment of mKate2 to produce an enhanced FusionRed-like red fluorescent protein

The paper describes a new red fluorescent protein variant that outperforms its parents in brightness, fluorescence lifetime, and blinking properties.

Redox-dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells

This study identifies redox-dependent binding configurations of NAD(P)H in live cells using FLIM and modeling, revealing how redox states influence fluorescence decay.

The ratio between experimental and theoretical localization precision of single molecules is sensor-dependent and typically larger than one

The paper examines the discrepancy between theoretical and experimental localization precision in single-molecule imaging, finding that experimental precision is often higher than theoretical estimates.

High speed functional imaging with a microfluidics-compatible open-top light-sheet microscope enabled by model predictive control of a tunable lens

This paper introduces a microfluidics-compatible open-top light-sheet microscope using model predictive control of a tunable lens to enable high-speed 3D imaging of C. elegans.

Single-molecule live imaging of subunit interactions and exchange within cellular regulatory complexes

This study uses PAPA-fSMT to investigate P-TEFb interactions and exchange in live cells, revealing dynamics of the 7SK ribonucleoprotein complex and its association with chromatin and BRD4.

AlliGator: Open Source Fluorescence Lifetime Imaging Analysis in G

AlliGator is a Windows open source software for analyzing fluorescence lifetime imaging data, offering FRET analysis and lifetime-derived quantity mapping.

Chemogenetic and optogenetic strategies for spatiotemporal control of split-enzyme-based calcium recording

This paper introduces two strategies to control the timing and location of Ca2+ recording using the SCANR system: one optogenetic and one chemogenetic.

Decoding mEos4b Day-Long Maturation and Engineering Fast Maturing Variants

The paper characterizes the maturation speed of mEos-derived PCFPs in E. coli and develops fast-maturing variants of mEos4b.

Self-supervised generation of realistic training data enables nanoscale localization in challenging conditions

The paper introduces a physics-informed generative model to create realistic training data for localization microscopy, improving performance in challenging conditions.

mScarlet3-H with low brightness and fluorescence lifetime has potential for cellular lifetime-unmixing and lifetime-based pH-sensing applications

This paper evaluates mScarlet3-H, a red fluorescent protein with lower brightness and fluorescence lifetime, showing its potential for lifetime-based pH sensing and cellular lifetime unmixing.

A gentle palette of plasma membrane dyes

This paper introduces PK Mem dyes for time-lapse fluorescence imaging, showing reduced phototoxicity and improved photostability for live and fixed cell imaging.

4polar3D : Single molecule 3D orientation imaging of dense actin networks using ratiometric polarization splitting

The paper introduces 4polar3D, a method for 3D orientation imaging of dense actin networks using ratiometric polarization splitting, demonstrating its ability to resolve nanoscale organization in cellular structures.

Beyond Static Screens: optical pooled screening of signaling dynamics using time-lapse FLIM

The paper develops a high-throughput live-cell screening platform using time-lapse FRET-FLIM to study dynamic signaling phenotypes, demonstrating its effectiveness in identifying regulators of cAMP signaling.

MUFASA: A Continuous-Time Stochastic Framework for Realistic Fluorescence Microscopy Simulation

MUFASA simulates fluorescence microscopy data using continuous-time Markov chains and provides parameter estimation and validation tools for SMLM and FF-SRM protocols.

Super-resolution microscopy of mitochondrial mRNAs

This study uses super-resolution microscopy to visualize mitochondrial mRNAs and their interactions with proteins, revealing dynamic changes in mRNA distribution and structure during apoptosis and in disease.

Fluorescent protein tags for human tropomyosin isoform comparison

The paper develops fluorescent tags to visualize and compare eight human tropomyosin isoforms in live cells without disrupting their function.

A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo

The paper introduces a genetically encoded fluorescence sensor for PKC activity, demonstrating its use in vivo and identifying PKC activity in specific neurons during locomotion.

Electrochemically controlled switching of dyes for enhanced super-resolution optical fluctuation imaging (SOFI)

This paper demonstrates electrochemical control of dye switching to enhance SOFI imaging resolution and speed by reducing switching heterogeneity and increasing switching rates.