Cellular transport and secretion

Multifunctional Roles of Sec13 Paralogues in the Euglenozoan Trypanosoma brucei

This study shows TbSec13.2 in Trypanosoma brucei is essential for COPII-mediated ER export, challenging the idea that it is restricted to the SEA/GATOR complex.

Ca2+ binding to Esyt is required to modulate membrane contact site density in Drosophila photoreceptors

This study shows that Ca2+ binding to dEsyt modulates ER-PM MCS density in Drosophila photoreceptors, linking Ca2+ influx to signal transduction.

Super-resolution single molecule network analysis (SuperResNET) detects changes to clathrin structure by small molecule inhibitors

SuperResNET analyzes single-molecule imaging data to show that Pitstop 2 and Dynasore alter clathrin structure in distinct ways in HeLa cells.

γ-secretase facilitates retromer-mediated retrograde transport

This study shows that γ-secretase interaction with retromer enhances retromer-mediated retrograde transport of specific cargos in human cells.

Arf family GTPases are present in Asgard archaea

This study identifies ArfR GTPases in Asgard archaea, showing they are ancestral to eukaryotic Arf family proteins and suggest early evolution of endomembrane systems.

GRASP55 Regulates Sorting and Maturation of the Lysosomal Enzyme β-Hexosaminidase A

This study shows GRASP55 regulates lysosomal enzyme trafficking by affecting HEXA sorting, M6P receptor interaction, and GNPTAB expression, leading to altered enzyme maturation and secretion.

Protein Kinase C positively regulates peroxisome biogenesis by promoting peroxisome-endoplasmic reticulum interaction

This study identifies PKC as a regulator of peroxisome biogenesis by enhancing peroxisome-ER interactions through GSK3β inactivation, particularly during neuronal differentiation.

A distant TANGO1 family member promotes vitellogenin export from the ER in C. elegans

This study identifies TNGL-1 as a C. elegans TANGO1 family member that facilitates vitellogenin export from the ER by engaging cargo via a luminal domain.

Actomyosin and the Arp2/3 Complex Are Involved in the Internalization of Cellulose Synthase Complexes

The study directly visualizes CSC internalization and shows actomyosin and the Arp2/3 complex are involved in both exocytosis and internalization of CSCs.

Synaptic vesicle characterization of iPSC-derived dopaminergic neurons provides insight into distinct secretory vesicle pools

The paper characterizes synaptic vesicles in iPSC-derived dopaminergic neurons, revealing distinct secretory vesicle pools with different molecular and ultrastructural features.

Acute GARP depletion disrupts vesicle transport leading to severe defects in sorting, secretion, and O-glycosylation

This study shows that acute GARP depletion causes vesicle transport defects, leading to mislocalization of Golgi proteins, O-glycosylation issues, and accumulation of vesicle-like structures.

BORC segregates synaptic vesicle and lysosomal proteins through motors UNC-104/KIF1A and UNC-116/KIF5

This study identifies BORC's role in segregating synaptic vesicle and lysosomal proteins in neuronal cell bodies through motors UNC-104/KIF1A and UNC-116/KIF5, with LRK-1 and APB-3 regulating this process.

A lever hypothesis for Synaptotagmin-1 action in neurotransmitter release

This paper proposes a lever hypothesis for Synaptotagmin-1 action in neurotransmitter release, suggesting it pulls SNARE complexes to facilitate membrane fusion.

Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons

The paper develops POTATOMap to study biosynthetic lysosomal protein trafficking in neurons, revealing domain-specific mechanisms and interactions.

TAT-1, a phosphatidylserine flippase, affects molting and regulates membrane trafficking in the epidermis of C. elegans

This study identifies TAT-1 as a phosphatidylserine flippase required for normal endosomal function and molting in C. elegans, linking it to NEKL-2/3 and endocytic processes.

Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1

This study shows that mutations in Syt1's C2 domains impair DCV docking but increase fusion pore size and serotonin release speed.

A structure-based mechanism for initiation of AP-3 coated vesicle formation

This study determines the structural mechanism of AP-3 membrane recruitment and activation through cryo-EM, revealing conformational changes driven by Arf1 binding and cargo engagement.

Molecular basis for plasma membrane recruitment of PI4KA by EFR3

The paper reports a cryo-EM structure of EFR3A bound to the PI4KA complex, revealing the molecular mechanism of PI4KA membrane recruitment and its regulation in human disease.

Segregated localization of target-SNARE proteins within presynaptic terminals of Munc18-1 deficient photoreceptors

The paper investigates the localization of SNARE proteins in Munc18-1 deficient photoreceptors and finds that Munc18-1 prevents segregation of syntaxin-3 and SNAP-25 in presynaptic terminals.

Ribosome-binding protein 1, RRBP1, maintains peroxisomal biogenesis

This study identifies RRBP1 as a novel peroxisome biogenesis factor in human cells, showing it promotes peroxisome formation and stabilizes peroxisomal proteins.

Putative neofunctionalization of a Poales-specific EXO70 clade

The paper investigates the neofunctionalization of a Poales-specific EXO70 clade, showing HvEXO70FX12 lost exocyst complex association and may play a role in immunity.

Epiboly in zebrafish requires reactive oxygen species produced by NADPH oxidases for the regulation of vesicular trafficking

This study shows that NADPH oxidase-derived reactive oxygen species regulate endocytosis and vesicular trafficking during zebrafish epiboly, affecting E-cadherin localization and embryo development.

A Commander-independent function of COMMD3 in endosomal trafficking

The paper identifies a Commander-independent function of COMMD3 in endosomal trafficking, mediated by its N-terminal domain binding and stabilizing ARF1.

SNARE mimicry by the CD225 domain of IFITM3 enables regulation of homotypic late endosome fusion

The paper identifies a SNARE-like motif in the CD225 domain of IFITM3 that modulates SNARE complex assembly and endosomal fusion, revealing a mechanism for its antiviral activity.

The two crystal structures of unloaded and cargo-loaded Vacuolar Sorting Receptor 1 lumenal domain (VSR1-Nt) reveal two cargo binding sites and a mechanism of transport.

The paper determines the crystal structures of unloaded and cargo-loaded VSR1-Nt, revealing two cargo binding sites and a calcium-dependent mechanism for cargo binding and release.

Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons

This paper develops Endo-IP and Lyso-IP methods to profile endolysosomal proteins in induced neurons, revealing cargo and machinery involved in neurodegenerative disease.

Structural basis for Rab6 activation by the Ric1-Rgp1 complex

The paper determines the cryoEM structure of the Ric1-Rgp1-Rab6 complex, revealing its mechanism for Rab6 activation and identifying a novel RabGEF domain and arrestin fold interactions.

Movement of the endoplasmic reticulum is driven by multiple classes of vesicles marked by Rab-GTPases

The paper identifies Rab6- and Rab1-marked vesicles as drivers of ER movement via hitchhiking, revealing multiple Rab-vesicle classes contribute to ER motility.

An RNAi screen of Rab GTPase genes in C. elegans reveals that somatic cells of the reproductive system depend on rab-1 for morphogenesis but not stem cell niche maintenance

This study uses RNAi screening in C. elegans to identify rab-1 as essential for gonad morphogenesis but not stem cell niche maintenance.

Coatomer complex I is required for the transport of SARS-CoV-2 progeny virions from the endoplasmic reticulum-Golgi intermediate compartment

This study identifies COPI as essential for SARS-CoV-2 virion transport from the ERGIC, showing that COPB2 depletion inhibits viral replication.