Cellular transport and secretion

Molecular architecture of synaptic vesicles.

This study uses cryo-electron tomography to describe the molecular architecture and structural heterogeneity of individual synaptic vesicles in mouse brains and cultured neurons.

PIKFYVE inhibition induces endosome- and lysosome-derived vacuole enlargement via ammonium accumulation

This study shows that PIKFYVE inhibition causes endosome and lysosome enlargement through ammonium accumulation from glutamine metabolism, impairing lysosomal function and autophagic flux.

Phosphorylation on serine 72 modulates Rab7A palmitoylation and retromer recruitment

This study shows that phosphorylation of Rab7A at serine 72 modulates its palmitoylation and retromer recruitment, with NEK7 as the kinase responsible for this phosphorylation.

Key determinants of the dual clamp/activator function of Complexin

This study identifies key molecular features of Complexin II that regulate vesicle fusion timing by interacting with SNARE proteins and synaptotagmin I.

Structural basis for Retriever-SNX17 assembly and endosomal sorting

This study determines the structure of the Retriever-SNX17 complex using cryo-EM, revealing how SNX17 interacts with Retriever to facilitate endosomal sorting of cargo proteins.

Intersectin1 promotes clathrin-mediated endocytosis by organizing and stabilizing endocytic protein interaction networks

This study shows that intersectin1 promotes clathrin-mediated endocytosis by organizing and stabilizing endocytic protein interaction networks rather than initiating CME.

Unremodeled plasma membrane GPI-anchored proteins trigger aberrant clathrin-mediated endocytosis

The paper shows that unremodeled GPI-anchored proteins in yeast cause lipid disorder and abnormal endocytosis, disrupting membrane protein trafficking.

Membrane fusion reactions limited by defective SNARE zippering or stiff lipid fatty acyl composition have distinct requirements for Sec17, Sec18, and adenine nucleotide

This study investigates how defective SNARE zippering or stiff lipid fatty acyl composition affect membrane fusion and the roles of Sec17 and Sec18 in overcoming these impediments.

Subtleties in Clathrin Heavy Chain Binding Boxes provide selectivity among Adaptor Proteins of Budding Yeast

The paper investigates clathrin binding selectivity among yeast adaptor proteins, finding Ent5 has the highest affinity and Ent1 shows stronger clathrin interaction than Ent2.

TC10 differently controls the dynamics of Exo70 in growth cones of cortical and hippocampal neurons

This study investigates how TC10 regulates Exo70 dynamics in growth cones of cortical and hippocampal neurons using super-resolution microscopy.

Mannose receptor (MRC1) mediates uptake of dextran in macrophages via receptor-mediated endocytosis

This study identifies MRC1 as a key receptor for dextran uptake in macrophages via receptor-mediated endocytosis, distinguishing it from fluid-phase uptake mechanisms.

Phospholipid signaling and multivesicular endolysosomes modulate membrane dynamics at the biotrophic interface in Rice Blast

This study identifies Rab7 and phosphoinositide signaling as critical for unconventional secretion of cytoplasmic effectors across the Magnaporthe oryzae-Rice interface during biotrophy.

E3 Ubiquitin Ligase Highwire/Phr1 Mediates Endocytic Control of JNK Signaling in Drosophila Neurons

This study identifies Highwire/Phr1 as an endocytosis-regulated E3 ubiquitin ligase that controls JNK signaling and synaptic growth in Drosophila neurons.

Rab11 suppresses neuronal stress signaling by localizing Dual leucine zipper kinase to axon terminals for protein turnover.

This study shows Rab11 suppresses neuronal stress by localizing DLK to axon terminals for protein turnover, and its loss leads to neuronal stress and loss.

Defects in exosome biogenesis are associated with sensorimotor defects in zebrafish vps4a mutants

This paper identifies a zebrafish vps4a mutation causing sensorimotor defects by reducing Vps4a ATPase activity and exosome biogenesis, affecting specific reflexes and vision.

Nance-Horan Syndrome-like 1 interacts with endophilin A2 and Ena/VASP proteins to promote fast endophilin-mediated endocytosis.

This study identifies NHSL1's role in promoting fast endophilin-mediated endocytosis by interacting with EndoA2 and Ena/VASP proteins, enhancing actin polymerization at FEME sites.

Growing Macrophages Regulate High Rates of Solute Flux by Pinocytosis

This study shows that murine macrophages under CSF1 stimulation rely on pinocytosis for solute influx and growth, with leucine affecting both protein synthesis and solute accumulation.

A distinctive PI(4,5)P2 compartment forms during entosis and related engulfment processes

The paper identifies a unique PI(4,5)P2-positive compartment formed during entosis and related engulfment processes, distinct from phagosomes and macropinosomes.

Neosynthesized polarized and non-polarized plasma membrane cargoes follow distinct trafficking routes in Aspergillus nidulans

The paper investigates distinct trafficking routes for polarized and non-polarized membrane proteins in Aspergillus nidulans, showing they use different secretory pathways and Golgi dependencies.

Rab6a enables BICD2/dynein-mediated trafficking of human papillomavirus from the trans-Golgi network during virus entry

This study identifies Rab6a as essential for HPV trafficking from the TGN to the nucleus by facilitating its association with dynein and BICD2.

Rabphilin-3A negatively regulates neuropeptide release, through its SNAP25 interaction

This study identifies rabphilin-3A as a negative regulator of DCV exocytosis through its interaction with SNAP25, showing increased DCV release in its absence.

PI(3,5)P2 asymmetry during mitosis is essential for asymmetric vacuolar inheritance

The Endoplasmic Reticulum as an Active Liquid Network

This paper develops a physical model of the endoplasmic reticulum as an active liquid network to explain its dynamic structure and function.

Filopodia-like Structures are Essential for Steroid Release

The paper identifies filopodia-like structures, called hormonemes, as essential for regulated steroid release in Drosophila, showing their role in synchronizing secretion with physiological needs.

Multiplexed DNA-PAINT Imaging of the Heterogeneity of Late Endosome/Lysosome Protein Composition

This study uses multiplexed DNA-PAINT imaging to reveal cell-type specific subpopulations of late endosomes/lysosomes with unique protein compositions.

Endosomal actin branching, fission and receptor recycling require FCHSD2 recruitment by MICAL-L1

This study identifies MICAL-L1's role in recruiting FCHSD2 to endosomes, which is essential for actin branching, fission, and receptor recycling.

Neurotransmitter release is triggered by a calcium-induced rearrangement in the Synaptotagmin-1/SNARE complex primary interface

This study identifies two distinct regions in the synaptotagmin-1/SNARE complex interface that mediate different functions in neurotransmitter release and shows that Ca2+ triggers a rearrangement of this interface.

Membrane tension regulation is required for wound repair

This study identifies membrane tension as a key regulator of exo- and endocytic events during plasma membrane wound repair, showing its role in coordinating resealing and homeostasis restoration.

Regulation of angiogenesis by endocytic trafficking mediated by cytoplasmic dynein 1 light intermediate chain 1

This study shows that LIC1 and RILPL1/2 restrict angiogenesis by promoting VEGFR2 degradation, and their disruption increases angiogenesis through enhanced Rab11-mediated recycling.

Active morphodynamics of intracellular organelles in the trafficking pathway

The paper investigates the morphodynamics of intracellular organelles by modeling active mechano-chemical cycles in trafficking pathways, showing how fission and fusion events generate active stresses and hydrodynamic flows.