Viral Infections and Outbreaks Research

Acute malaria dysregulates specialized lymph node macrophages to suppress vaccine-elicited protection against Ebola virus

This study shows that acute malaria infection reduces the effectiveness of a low-dose Ebola vaccine by impairing antibody responses in lymph node macrophages, which can be mitigated with higher vaccine doses.

Novel Mechanism of Inflammatory Activation by Ebola Virus Matrix Protein Linked to the Ebolavirus Virulence

This study identifies VP40 as a key virulence factor in Ebola virus that drives pro-inflammatory responses through NF-κB activation in non-immune cells, independent of TLR4.

Assessing Ebola Virus Circulation in the Tshuapa Province (Democratic Republic of the Congo): A One Health Investigation of Wildlife and Human Interactions

This study investigates Ebola virus circulation in wildlife and human interactions in the Democratic Republic of the Congo, finding antibodies in wildlife and humans but no active virus, suggesting seasonal spillover risks.

Evidence of latency reshapes our understanding of Ebola virus reservoir dynamics

This study models latency in Ebola virus using a novel phylogenetic approach, finding evidence of extended quiescence in the reservoir, which changes understanding of its evolutionary history and geographic spread.

Conserved Filovirus Proteins as Targets of Broad-Spectrum Antivirals

This study identifies conserved binding sites in filovirus proteins to guide the development of broad-spectrum antivirals, finding high conservation in L and VP40 proteins and validating existing drugs like Remdesivir.

Molecular insights into nucleocapsid assembly and transport in Marburg and Ebola viruses

The paper identifies key nucleocapsid proteins in Marburg and Ebola viruses and shows their role in nucleocapsid assembly and transport, highlighting a conserved motif as a potential antiviral target.

Ebola virus VP35 NNLNS motif modulates viral RNA synthesis and MIB2-mediated signaling

This study identifies and characterizes the NNLNS motif in Ebola virus VP35 that mediates MIB2 binding, inhibiting IFN production and affecting viral RNA synthesis.

Egyptian rousette bat macrophages elicit divergent interferon responses and cytokine storm signalling against Marburg and Sudan viruses

This study compares macrophage responses to Marburg and Sudan viruses in Egyptian rousette bats, revealing divergent interferon and cytokine storm signaling pathways.

Proteolytic processing of the Marburg virus glycoprotein depends on Sec616β and is required for cell entry

This paper identifies Sec61β as essential for proteolytic processing and cell entry of Marburg virus glycoprotein, unlike Ebola virus, and suggests Sec61 as a therapeutic target.

Wisteria floribunda agglutinin enhances Zaire ebolavirus entry through interactions at specific N-linked glycosylation sites on the virus glycoprotein complex

Ebola's Hidden Target: Virus Transmission to and Accumulation within Skin

This study investigates how Ebola virus infects and accumulates in skin tissues of infected animals, identifying TIM-1 as a critical host receptor for viral dissemination.

Molecular basis for the increased membrane fusion activity of the Ebola virus glycoprotein A82V variant from the 2013-2016 epidemic: insights from simulations and experiments

This study identifies how the A82V mutation in Ebola virus GP enhances membrane fusion through conformational changes, using simulations and experiments.

Jamaican fruit bats (Artibeus jamaicensis) competence for Ebola virus but not Marburg virus is driven by intrinsic differences in viral entry and IFN-I signaling antagonism.

This study shows Jamaican fruit bats can support EBOV replication and shedding but not MARV, due to differences in viral entry and IFN-I signaling suppression.

Evaluating the impact of NPC1 SNPs on entry efficiency of Filovirus in vitro: agent-based model approach

The paper uses an agent-based model to quantify how specific NPC1 SNPs affect filovirus entry efficiency in vitro, finding that D508N, P424A, and S425L reduce entry efficiency for both Ebola and Marburg viruses.

Rational design of next-generation filovirus vaccines through glycoprotein stabilization, nanoparticle display, and glycan modification

This study rationally designs and characterizes filovirus glycoproteins and nanoparticles in vitro and in vivo to aid vaccine development.

Metabolically purified human stem cell-derived hepatocytes reveal distinct effects of Ebola and Lassa viruses

This study uses metabolically purified human hepatocytes to show that Ebola and Lassa viruses have distinct effects on liver cells.

A Multivalent Pan-Ebolavirus Nanoparticle Vaccine Provides Protection in Rodents from Lethal Infection by Adapted Zaire and Sudan Viruses

This study develops a multivalent nanoparticle vaccine against EBOV and SUDV, showing protection in rodents from lethal infection.

Multiple dermal cell types support productive infection and dynamic translocation of infectious Ebola virus to the apical surface of human skin

This study identifies dermal cell types that support Ebola virus infection and shows how the virus traffics to the skin surface.

Genomic characterization uncovers transmission dynamics of Marburg Virus in Rwanda following a single zoonotic spillover event

The paper uses genomic analysis to show the Rwanda MVD outbreak originated from a single zoonotic event with limited human-to-human transmission.

Ebola virus infection of Flt3-dependent, conventional dendritic cells and antigen cross-presentation leads to high levels of T-cell activation

This study shows that Ebola virus infection of Flt3-dependent conventional dendritic cells enhances cross-presentation, leading to increased CD8 T-cell activation in severe EVD.

A viral vaccine design harnessing prior BCG immunization confers protection against Ebola virus

This paper demonstrates a viral vaccine strategy using BCG-induced Th cells to enhance antibody responses against Ebola virus, providing protection in a mouse model.

Impact of Ebola virus nucleoprotein on VP40 virus-like particle production: a computational approach

This study uses computational methods to show that NP enhances VP40 VLP production by stabilizing filaments and accelerating budding, with optimal timing and expression ratios.

Evaluation of Fendiline Treatment in VP40 System with Nucleation-Elongation Process: A Computational Model of Ebola Virus Matrix Protein Assembly

This paper evaluates the effect of fendiline on EBOV VP40 filament assembly using a computational model, finding it may temporarily increase VLP production but generally reduces it.

Measuring the unknown: an estimator and simulation study for assessing case reporting during epidemics

The paper introduces a statistical method to estimate case reporting during epidemics using transmission chain data and evaluates its performance through simulations.

Mechanisms of phosphatidylserine influence on viral production: a computational model of Ebola virus matrix protein assembly

This study uses a computational model to investigate how phosphatidylserine influences VP40 matrix protein assembly in Ebola virus, showing PS affects filament growth and assembly steps.

Rescue Ebola Patients in silico

The paper develops a mathematical model of the immune response to Ebola infections to stratify patients and identify potential treatment strategies.

A live-cell imaging system for visualizing the transport of Marburg virus nucleocapsid-like structures

The paper develops a live-cell imaging system to visualize Marburg virus nucleocapsid-like structures outside BSL-4 labs, showing they transport similarly to actual nucleocapsids.

Machine-Learning prognostic models from the 2014-16 Ebola outbreak: data-harmonization challenges, validation strategies, and mHealth applications

The paper develops machine-learning prognostic models for Ebola virus disease using data from the 2014-16 outbreak, validated across independent datasets and integrated into a mobile health app.

Pan-filovirus one-step reverse transcription-polymerase chain reaction screening assay

The paper develops a pan-filovirus RT-PCR assay to detect all known filovirus species using universal primers targeting conserved regions of the nucleoprotein.

Positive selection in the Ebola virus proteome

The paper analyzes amino acid substitutions in Ebola virus proteomes to identify evolutionary hotspots and positive selection across different lineages and outbreaks.