Hemoglobin structure and function

A dichotomous family of Cache domain proteins evolved through c-type heme acquisition

Cache sensor domains of DUF3365/Tll0287 family bind covalent c-type heme through a CXXCH insertion; a signaling member behaves like an NO sensor, a stand-alone one like a redox protein.

Origins of allostery in vertebrate hemoglobin evolution

This study shows that allostery in vertebrate hemoglobin evolved from a non-allosteric dimer through three key substitutions, leveraging inherent conformational flexibility of the globin fold.

Mechanism of heme binding by CP motifs in the BACH1 DNA-binding region

This study investigates how heme binds to BACH1's CP motifs and how this affects DNA dissociation, revealing that multiple residues contribute to heme binding and DNA release.

Structural Basis of a Novel Heme Binding Bacterial One-Component Switch

This paper characterizes FG214, a heme-binding one-component switch from Fimbriimonas ginsengisoli, showing heme redox state controls monomer-dimer transitions and DNA binding.

Identification of scavenger receptor BI as a scavenger of free heme that is essential for protection against hemolysis

This study identifies scavenger receptor BI as a critical heme scavenger that protects against hemolysis, showing its therapeutic potential in hemolytic disorders.

Immobilized neuroglobin scavenges carbon monoxide from circulating carboxyhemoglobin

This paper develops an immobilized neuroglobin-based system for CO removal, showing preserved activity and recyclability in a solid matrix.

Regulatory mechanism of heme-regulated inhibitor through autophosphorylation-driven activation and heme-induced deactivation

This study identifies autophosphorylation-driven activation and heme-induced deactivation mechanisms of HRI, revealing how phosphorylation and heme regulate its interaction with eIF2.

Cryo-EM provides insight into how the Staphylococcus aureus IsdH receptor removes hemin from the hemoglobin:haptoglobin complex

This paper uses cryo-EM to show how the Staphylococcus aureus IsdH receptor extracts hemin from the hemoglobin:haptoglobin complex by selectively engaging βHb and using N-linked glycans on haptoglobin to facilitate this process.

The Rationalization of Carbon Monoxide and Hemoglobin Association

This paper derives equations describing the relationship between carbon monoxide saturation and pressure, showing a hyperbolic curve and confirming CO's 230-fold higher affinity for hemoglobin than oxygen.

A Deep Dive into the Globin Superfamily of Sharks, Skates, and Rays: Contrasting patterns of gene loss and retention relative to bony vertebrates

This study analyzes globin gene evolution in cartilaginous fish using genomic and transcriptomic data, revealing gene loss, retention, and expression patterns compared to bony vertebrates.

Hemoglobin alpha regulates T-lymphocyte activation and mitochondrial function

This study identifies hemoglobin alpha's role in T-lymphocyte activation and mitochondrial function, showing its impact on cytokine production and EAE severity.

Cytochrome b5 reductase 4 efficiently reduces Neuroglobin and Cytoglobin

This study shows that cytochrome b5 reductase 4 can efficiently reduce both cytoglobin and neuroglobin, suggesting it may serve as a neuroglobin reductase in vivo.

Arterial iron regulates vasodilation during anemia via endothelial holo-alpha globin

This study shows arterial iron deficiency affects vasodilation by altering endothelial holo-alpha globin levels and NO signaling in female mice, but not in males.

Full-length structure and heme binding in the transcriptional regulator HcpR

This study determines the structure of HcpR from Porphyromonas gingivalis and identifies key residues involved in heme binding and activation.

Deep Red Blood Cell Proteome Defines the Band 3 N-Terminus Interactome as a Regulator of Hypoxic Adaptation via BLVRB-Dependent S-Nitroso Transfer

The paper defines the Band 3 N-terminus interactome in red blood cells, revealing its role in hypoxic adaptation through BLVRB-dependent S-nitroso transfer and metabolic remodeling.

Myoglobin leaching into the serum of IDA mice is driven by the high activation of sGC under anemic conditions which induces myoglobin expression

This study shows that myoglobin leaks into the serum of anemic mice due to increased sGC activation and expression, which is linked to iron deficiency anemia.

Signatures of Electron-hole Hopping in Myoglobin Peroxidase Activity Revealed by Deep Mutational Learning

This study uses deep mutational learning to identify myoglobin variants with enhanced peroxidase activity, showing the role of electron-hole hopping in catalytic efficiency.

Cell-Free production of soybean leghemoglobins and non-symbiotic hemoglobin

The paper demonstrates the production of soybean leghemoglobins and non-symbiotic hemoglobins using E. coli-based cell-free protein synthesis, showing correct amino acid sequence, functionality, and digestibility.

Engineering a highly selective, hemoprotein-based scavenger as a carbon monoxide poisoning antidote with no hypertensive effect

This paper develops a hemoprotein-based CO scavenger that selectively removes CO from hemoglobin, accelerates its clearance, and avoids hypertension compared to hemoglobin-based treatments.

Scavenger receptor CD163 multimerises to allow uptake of diverse ligands

This study shows CD163 forms multimeric complexes to recognize diverse haemoglobin-haptoglobin ligands, enabling detoxification.

Structural elucidation of the haptoglobin-hemoglobin clearance mechanism by macrophage scavenger receptor CD163

This study determines the cryo-EM structures of CD163 in complex with haptoglobin-hemoglobin, revealing its trimeric structure and calcium-dependent mechanism for HpHb clearance.

Conformational analysis of liganded human hemoglobin by cryo electron microscopy

This study uses cryo-EM to analyze the conformational states of CO-bound human and crocodile hemoglobin, revealing distinct structural characteristics and the influence of an amino acid substitution.

Two birds with one stone: a novel potential antibiotic blocking IsdB-mediated heme extraction by Staphylococcus aureus with serendipitous hemoglobin left-shifting activity

This paper identifies C35 as a dual-acting compound that inhibits Staphylococcus aureus growth by blocking heme acquisition and modulates hemoglobin oxygen affinity.

Rational design of tertiary coordination sphere of a heme-based sensor for two-orders enhanced oxygen affinity

This study enhances the oxygen affinity of the DosS protein by modifying its heme coordination sphere, achieving a 150-fold increase in O2 binding affinity.

The Multifunctional Catalytic Hemoglobin from Amphitrite ornata: Protocols on Isolation, Taxonomic Identification, Protein Extraction, Purification, and Characterization

This paper describes a method for taxonomic identification and characterization of the multifunctional catalytic hemoglobin (AoDHP) from Amphitrite ornata, including DNA barcoding and enzyme activity assays.

Hemoglobin alpha is a redox-sensitive mitochondrial-related protein in T-lymphocytes

This study identifies hemoglobin alpha-a1 as a redox-sensitive mitochondrial antioxidant in T-lymphocytes, showing its regulation by redox stress and metabolic state.

Evolution of myoglobin in avian lineages: positive selection for aggregation resistance in penguins and high purifying selection in galliforms

The paper models myoglobin structures in 302 vertebrates, finds positive selection for aggregation resistance in penguin myoglobins and purifying selection in galliforms, and proposes a new equation for net charge and concentration relationship.

Molecular basis of hemoglobin adaptation in the high-flying bar-headed goose

The paper investigates the molecular basis of hemoglobin adaptation in bar-headed geese by analyzing mutational intermediates and their pleiotropic effects.

Allosteric signalling paths in hemoglobin: a protein dynamics network analysis

This study maps heme-heme interactions in hemoglobin using molecular dynamics simulations to identify key residues and pathways involved in allosteric signaling.