flow cytometry

Heterogeneity in cell-cycle dynamics of synthetic mRNA-induced β-cell proliferation

This study induces β-cell proliferation in rats using synthetic mRNAs and identifies heterogeneity in cell-cycle dynamics, revealing variability in G1 duration and division completion.

Adenosine impairs T cell function via activation of purine salvage pathway and AMP-induced inhibition of pyrimidine nucleotide biosynthesis

This study shows adenosine impairs T cell function by activating the purine salvage pathway and inhibiting pyrimidine biosynthesis through AMP-mediated suppression of UMP synthase.

Phenotypic and functional characterization of tumor-reactive T cells in malignant pleural effusions

This study characterizes T cells in malignant pleural effusions (MPE) and finds they have lower exhaustion and higher cytotoxic potential compared to tumor T cells, suggesting MPE as a better source for TIL therapy.

Individual mouse mitotic chromosomes exhibit cell type-specific differences in biomechanical properties

The paper shows that mouse metaphase chromosomes from different cell types have distinct biomechanical properties, with ESC-derived chromosomes showing conserved force-dependent responses but differing from those of NSCs and preB cells.

A Full Spectrum Fibroblast Profiling Panel Reveals Phenotypic and Metabolic Heterogeneity of Cancer-Associated Fibroblasts at the Single-Cell Level

This paper introduces a 28-marker flow cytometry panel to profile cancer-associated fibroblasts (CAFs) and reveals their phenotypic and metabolic heterogeneity across different models.

Natural killer cells mediated antibody dependent cellular cytotoxicity response in acute and recovery phases of chikungunya

The paper assesses NK cell-mediated ADCC response in chikungunya patients across different phases and finds persistent ADCC in recovered individuals and correlation with antibody levels.

Development and translational validation of a novel mouse model for allergic rhinitis

This paper develops a novel mouse model of cat allergic rhinitis that recapitulates key clinical features and enables mechanistic and therapeutic studies.

High-Dimensional Immunophenotyping Identifies Circulating Immune Signatures Associated with FeNO-Defined Asthma Phenotypes

This study identifies distinct systemic immune signatures in asthma patients based on FeNO levels using high-dimensional immunophenotyping.

Machine Learning Approach for Enumeration of Circulating Cells with Diffuse in vivo Flow Cytometry

The paper develops a machine learning approach to improve CTC enumeration in diffuse in vivo flow cytometry by distinguishing CTC peaks from artifacts.

Adaptive motility enables neutrophils to rapidly navigate confined capillaries

This study shows neutrophils maintain high migration speed in capillaries of varying sizes by adapting their actomyosin cytoskeleton via the ROCK-MyoII pathway, preventing vascular obstruction.

Circulating Immune Profiling Reveals Immune Signatures Associated with Disease Stage and Outcome in Endometrial Cancer

This study identifies immune signatures in circulating blood linked to disease stage and prognosis in endometrial cancer using flow cytometry.

Chronic opioid-associated immune dysregulation among people living with HIV

This study finds that opioid use disorder is linked to persistent immune dysregulation in HIV-positive individuals despite effective antiretroviral therapy.

A CD40-Targeted IL-21 Fusokine Enables Rapid Generation of Human IL-10⁺Granzyme B⁺ Regulatory B Cells

This paper demonstrates that a CD40-targeted IL-21 fusion protein and TLR9 activation can induce a stable regulatory B cell phenotype in human B cells with high efficiency.

Diabetes mellitus affects urinary tissue tropism of group B streptococci in a sex-dependent manner

This study shows diabetes increases GBS urinary tract infection susceptibility and dissemination, with sex-dependent differences in tissue tropism and immune response.

A 28-color panel for classical and non-classical T lymphocytes in decidua and PBMC in rhesus macaques

This paper develops a 28-color flow cytometry panel to identify T lymphocytes, B cells, and NK cells in rhesus macaque decidual and PBMC samples, enabling analysis of maternal immune responses during pregnancy.

Age and sex alter the immune response in a chronic fibrosis model via changes in T cell and macrophage phenotype

This study shows age and sex affect fibrosis and immune response in a mouse model of implant-induced chronic inflammation through changes in T cell and macrophage phenotypes.

Interferon-α-Driven Stratification of B Cell Reveals Metabolic Reprogramming of Double Negative, Naive and Transitional cell subsets and Refines Molecular Classification in Söjgren's Disease.

This paper identifies an IFN-α signature in B cells of Sjögren's disease patients, revealing metabolic reprogramming and refining molecular classification.

Systematic Evaluation of Nasal Immune Cell Sampling and Antigen-specific T cell Detection using Cryopreserved Nasal Swabs

This study evaluates nasal immune cell sampling methods and antigen-specific T cell detection using cryopreserved nasal swabs, finding that flocked swabs yield more cells and cryopreservation preserves functional T cells.

A 16-colour spectral flow cytometry panel to characterise T cell immunophenotypes in canine oral melanoma

The paper develops a 16-color spectral flow cytometry panel to analyze T cell immunophenotypes in canine oral melanoma and identifies distinct immune features in the tumor microenvironment.

A flow cytometry-based screening platform for identifying candidate radiosensitizers targeting DNA repair

This paper develops a flow cytometry-based screening platform to identify radiosensitizers targeting DNA repair, finding compounds that enhance radiosensitivity in cancer cells.

Larger inducible reservoir and higher abundance of exhausted CD8+ T cells in people treated after late versus non-late diagnosis of chronic HIV one year after ART initiation

SynNotch receptors for visualizing immunoreceptor force transmission and downstream signaling in vivo

This study develops SynNotch reporters to visualize force transmission and downstream signaling in immunoreceptors in vivo, showing force acts as a co-stimulus for CD40 and TCR signaling.

ALDH Activity in Monocytes is Associated with Subclinical Coronary Atherosclerosis in Treated People with HIV-1

The paper links ALDH activity in monocytes to subclinical coronary atherosclerosis in HIV-1 treated individuals, independent of traditional risk factors.

Hepatic γδ NKT cells modulate liver-resident CD8+ T cells to attenuated malaria parasite vaccines

This study identifies hepatic γδ NKT cells as modulators of liver-resident CD8+ T cell responses in malaria vaccine efficacy, showing their role in protection through IFNγ production and tissue-homing properties.

ST6GAL1-mediated sialyl linkage switching in tumor-associated macrophages drives cancer-promoting nanotubes carrying α2,6-sialylation in anti-inflammatory cells

This study identifies ST6GAL1-mediated sialyl linkage switching in TAMs that drives cancer-promoting nanotubes with α2,6-sialylation, affecting immune interactions and CRC progression.

A Shifting Immune Landscape: ILC Redistribution and Neutrophil Polarization in Vascular Cognitive Impairment and Dementia (VCID)

This study investigates how vascular dysfunction in VCID affects innate immune cells, finding compartment-specific ILC redistribution and pro-inflammatory neutrophil polarization in the brain.

Memory T Cells in MHC-Deficient Humanized Mice

This study compares T cell development in humanized mice with and without murine MHC, finding that busulfan conditioning in MHC-deficient mice promotes activated T cells while irradiation favors naive T cells.

Differential Impact of Isoflurane and Propofol on Apoptotic Regulation of Helper T cells

This study compares the effects of isoflurane and propofol on apoptosis in CD4+ T cells, showing isoflurane induces apoptosis via ROS-p38-caspase-3/7 pathway, while propofol protects mitochondrial function.

Ketone ester supplementation reduces age-associated B cell activation in aged mice without impairing antibody responses

Bidirectional disruption of Lrrk2 function drives T cell dysregulation and an exhaustion-like immune response

This study shows that disrupting Lrrk2 function in both directions leads to T cell dysregulation and exhaustion-like immune responses in mice.