Trypanosoma species research and implications

Trypanosoma congolense Variant Surface Glycoprotein gene expression occurs in the absence of monoallelic transcription control

This study shows that Trypanosoma congolense expresses multiple Variant Surface Glycoprotein genes in single cells, unlike Trypanosoma brucei, which uses monoallelic transcription.

Restricted expression site use and extreme genome diversification drives trypanosome antigenic variation in chronic bovine infections

The paper analyzes antigenic variation in Trypanosoma brucei during chronic bovine infections, revealing enhanced diversity and a reproducible temporal expression hierarchy of VSGs.

A platform for robust quantitative functional genomics reveals temporal fitness landscapes in African trypanosomes

DRiF-Seq, a sequencing-optimized RNAi platform with a posteriori noise estimation, quantifies clone-resolved fitness genome-wide in pleomorphic Trypanosoma brucei; over half of core genes carry fitness costs.

Structural basis for the inhibition of Trypanosoma brucei enolase by a camelid single-domain antibody

This paper identifies a camelid single-domain antibody that inhibits T. brucei enolase and elucidates its inhibitory mechanism using biochemical, biophysical, and structural methods.

Renal and endothelial biomarkers in Chagas disease in the Brazilian Amazon region: early indicators of kidney injury and disease progression

This study identifies elevated biomarkers in blood and urine that indicate early renal and endothelial dysfunction in Chagas disease patients in the Brazilian Amazon, undetectable by conventional tests.

Glycerol metabolism triggers trypanosome differentiation into transmissible forms in mammalian tissue-like conditions

This study identifies glycerol as a trigger for Trypanosoma brucei differentiation into transmissible forms under tissue-like conditions, challenging the traditional bloodstream model of transmission.

Acute Sublethal Heat Stress Impairs Blood Feeding and Trypanosome Infection in the Kissing Bug, Rhodnius prolixus

This study shows that acute heat stress reduces blood feeding and Trypanosome infection in Rhodnius prolixus, with recovery improving these outcomes.

Cyclic AMP compartmentalization drives signal specificity to control vector colonization and mammalian host infection by American trypanosomes

This study identifies two cAMP microdomains in Trypanosoma cruzi, showing their distinct roles in parasite survival and infection.

Uncovering parasite diversity in Ecuadorian wildlife: new trypanosomatid species and novel reservoir hosts for Leishmania amazonensis

Evaluation of Trypanosoma brucei Phosphofructokinase Allosteric Inhibition: An In-Silico Study

This study evaluates T. brucei phosphofructokinase inhibitors using in-silico methods, finding that inhibitor-ATP interactions predict inhibitory activity and that virtual screen compounds outperform existing ones.

Comparative pangenomics of Trypanosoma brucei reveal host specialization is driven by targeted or segmental genome streamlining in human and equine-restricted lineages

This paper investigates how Trypanosoma brucei adapts its genome during host specialization, revealing two distinct structural evolution pathways in human and equine-restricted lineages.

A Trypanosoma brucei gambiense gene variant confers human serum resistance

The paper identifies a gene variant in Trypanosoma brucei gambiense that confers resistance to human serum, explaining how this subspecies evades trypanolytic factors.

A genome wide antigen-antibody screen identifies a yeast-based therapeutic vaccine candidate for Chagas disease

The paper identifies a yeast-based therapeutic vaccine candidate for chronic Chagas disease by screening T. cruzi antigens with patient antibodies and validating its efficacy in mice.

From Proteome Mining to Structural Validation: Phosphopyruvate Hydratase as a Structurally Tractable Drug Target in Kinetoplastid Parasites

The paper identifies phosphopyruvate hydratase (PPH) as a drug target in kinetoplastid parasites through proteome mining, structural modeling, and experimental validation, and identifies hinokinin as a promising antiparasitic compound.

Trypanosoma brucei infection remodels the uterine immune environment and drives neuroendocrine dysfunction

This study shows that Trypanosoma brucei infection affects the uterine immune environment and causes neuroendocrine dysfunction in mice, with distinct effects on morphology and inflammation.

NEK kinase TcRDK2 controls differentiation, host-cell infection, and remodeling of the translation initiation machinery in Trypanosoma cruzi

This study identifies TcRDK2 as a NEK kinase essential for kinetoplast segregation, metacyclogenesis, and host-cell infection in T. cruzi, highlighting it as a potential therapeutic target for Chagas disease.

Distinct nanoscale membrane organizations of mucins and trans-sialidases in Trypanosoma cruzi

This study reveals the nanoscale organization of mucins and trans-sialidases in T. cruzi, showing segregated clusters and a shared fibrillar network with distinct structural features.

The bromodomain protein Bdf3 is sufficient to activate expression of a procyclin gene in bloodstream stage African trypanosomes

This paper shows that Bdf3 activation at the EP promoter in bloodstream Trypanosoma brucei increases EP1 transcript levels, supporting its role in initiating transcription during differentiation to the procyclic stage.

Integrative genomics identifies candidate genes underlying trypanotolerance in hybrid African cattle

This study identifies candidate genes for trypanotolerance in African cattle by integrating genomic and transcriptomic data from hybrid populations.

Reduced levels of inositol hexakisphosphate kinase (IP6K) impair life-cycle transitions and the intracellular development of Trypanosoma cruzi within human cardiomyocytes

This study shows that reducing IP6K levels in T. cruzi impairs key life-cycle transitions and intracellular development in human cardiomyocytes, suggesting IP6K as a potential drug target for Chagas disease.

Remodeling of tRNA modification in Trypanosoma cruzi life forms

This study profiles tRNA modifications in different life stages of Trypanosoma cruzi and finds that hydroxywybutosine modification levels regulate metacyclogenesis.

The Trypanosoma cruzi cell atlas; a single-cell resource for understanding parasite population heterogeneity and differentiation.

This paper creates a single-cell atlas of Trypanosoma cruzi, revealing life cycle genes, cell heterogeneity, and transcriptomic changes during differentiation.

Investigating the axoneme CCDC40 protein reveals new insights in trypanosome morphogenesis and division

This study investigates the role of CCDC40 in trypanosome flagellum structure and function, revealing that its depletion causes structural defects but not impaired cell division.

Adaptive metabolic reprogramming of the brain tissue during late-stage Trypanosoma brucei infection maintains host learning and memory

This study shows that during stage II of HAT infection, the brain reprograms its metabolism to use fatty acids and lipids instead of glucose, maintaining cognitive functions despite metabolic changes.

Cell surface localisation of GPI-anchored receptors in Trypanosoma brucei

This study shows GPI-anchored receptors in Trypanosoma brucei are localized across the entire cell surface, not just the flagellar pocket, challenging previous assumptions about immune evasion.

Nonlinear Dynamics of Left Ventricular Mass Remodeling in Chagas Cardiomyopathy

This study examines nonlinear dynamics of left ventricular mass remodeling in Chagas cardiomyopathy and links them to all-cause mortality using mathematical modeling and clinical data.

Loss of Vitellogenin Receptor Function Results in Yolk Depletion, Virome Expansion and Reduced Bacterial Load Within the Oocytes of Rhodnius prolixus

This paper shows that blocking the vitellogenin receptor in Rhodnius prolixus causes yolk depletion and changes in the oocyte microbiome, including reduced bacteria and increased viruses.

Transcriptomic profiling of embryo-derived cell lines from the Chagas disease insect vector Rhodnius prolixus

The paper characterizes the transcriptomes of two Rhodnius prolixus embryo-derived cell lines, identifying shared and distinct gene expression patterns that suggest different cell phenotypes.

A lipid droplet triacylglycerol lipase governs neutral lipids mobilization and differentiation of Trypanosoma brucei

This study identifies and characterizes TbPat, a triacylglycerol lipase in Trypanosoma brucei, showing its role in lipid mobilization and differentiation.

Systems-level analysis of RDK1 reveals compartment-specific kinase activity and a function in the maintenance of the mitochondrial proteome in Trypanosoma brucei

This study identifies RDK1's compartment-specific kinase activity and its role in maintaining the mitochondrial proteome in Trypanosoma brucei through proteomics and phosphoproteomics analyses.