Trypanosoma species research and implications

Structural modelling and biophysical analyses reveal a dimeric coiled-coil architecture in the FAZ10 central region of Trypanosoma brucei

This study determines the dimeric coiled-coil structure of the FAZ10 central region in Trypanosoma brucei using structural modeling and biophysical analysis.

A Genome-Wide Genetic Screen Identifies a Novel kDNA Replication Protein in Trypanosomes

This study uses a genetic screen in Trypanosoma brucei to identify novel kDNA maintenance factors, validates one as a novel replication factor, and provides a prioritized list of candidates.

Revisiting Cysteine Protease Function in Trypanosoma cruzi: Implications for Parasite Egress and Differentiation

This study investigates the role of cysteine proteases in Trypanosoma cruzi differentiation and egress, showing that their inhibition blocks maturation and delays parasite release.

The trypanosomatid dynamin-like protein associates with glycosomes

This paper identifies the trypanosomatid dynamin-like protein (DLP) as associating with glycosomes and shows its role in glycosome maintenance through knockdown and overexpression experiments.

Comparative lipidomic profiling of the livestock pathogens Trypanosoma congolense and Trypanosoma brucei

This study compares lipidomic profiles of Trypanosoma congolense and Trypanosoma brucei to understand their metabolic differences and implications for in vitro culture and therapy development.

Single-cell RNA-seq reveals trans-sialidase-like superfamily gene expression heterogeneity in Trypanosoma cruzi populations

This study uses single-cell RNA sequencing to show that T. cruzi trypomastigote cells express trans-sialidase-like superfamily genes with high heterogeneity and no recurrent combinations.

Structural Insights into Bromodomain-Containing Complexes from Trypanosoma cruzi Revealed by Proximity Labeling and Stoichiometric Space Exploration

The paper identifies the structure and stoichiometry of bromodomain-containing complexes in Trypanosoma cruzi using proximity labeling and structure prediction.

Specialised RNA decay fine-tunes monogenic antigen expression in African trypanosomes

The paper identifies three proteins that negatively regulate ESAG transcripts through specialised RNA decay in Trypanosoma brucei, revealing a novel post-transcriptional regulatory mechanism.

Germline Targeted Baboon Apolipoprotein L-1 Protects Mice Against African Trypanosomes

This study generates trypanosome-resistant murine models expressing primate APOL1 proteins to investigate their role in immunity, finding that baboon APOL1 provides partial protection but with limited expression, while a chimeric APOL1 variant shows protection with a fitness cost.

A Trypanosome Trifecta: an independently tunable triple inducer system for genetic studies in Trypanosoma brucei.

This paper develops a triple inducer system in Trypanosoma brucei for independently regulating three genetic elements, demonstrating independent control and tunability.

The trypanosome mRNA decapping enzyme ALPH1 prefers caps without m7G methylation and produces diphosphate-RNA

This study characterizes the trypanosome mRNA decapping enzyme ALPH1, showing it preferentially cleaves non-m7G-capped RNAs and produces diphosphate-RNA.

Glycosomal phosphoenolpyruvate carboxykinase CRISPR/Cas9-deletion and its role in Trypanosoma cruzi metacyclogenesis and infectivity in mammalian host

This study uses CRISPR/Cas9 to delete PEPCK in T. cruzi, showing it impairs differentiation and host invasion but enhances replication once inside host cells.

Live triatomine bug, vector of Trypanosoma cruzi (Chagas disease), found engorged in Lisbon hotel room: A first for Portugal and for Europe

The paper reports the first live triatomine bug in Europe, found in a Lisbon hotel room, highlighting the risk of accidental importation of disease vectors.

Advancing the design of the kissing bug kill trap for surveillance of triatomines

The paper tests improvements to a solar-powered trap for capturing kissing bugs, finding that a six-funnel design with specific lighting and placement is most effective.

Beyond the transcript: chromatin implications in trans-splicing in Trypanosomatids

The paper identifies trans-splicing acceptor sites in TriTryps and shows similar chromatin organization across genes, with histone-containing complexes protecting these sites.

Evolutionary Remodelling of a Remnant GET-Pathway Factor into PEX38, a Novel and Essential Peroxin in Euglenozoa

The paper identifies PEX38 as a novel, essential peroxin in Euglenozoa, evolved from a remnant GET-pathway factor, and highlights its role in peroxisomal membrane protein targeting and potential as a therapeutic target.

Defining the chromatin-associated protein landscapes on Trypanosoma brucei repetitive elements using synthetic TALE proteins

The paper uses synthetic TALE proteins to identify chromatin-associated proteins on repetitive elements in Trypanosoma brucei, revealing their roles in chromosome structure and function.

Comprehensive genomic analysis of Trypanosoma rangeli reveals key insights into the biology and evolution of this non-virulent American mammalian trypanosome

This study presents a near-complete genome of Trypanosoma rangeli using hybrid sequencing, transcriptomics, and proteomics, revealing novel insights into its biology and evolution.

Histone H3 Ser10 phosphorylation occurs exclusively in replicative stages and peaks during mitosis in Trypanosoma cruzi

Conjugates of α-d-Galp-(1->3)-β-d-Galp for the serological diagnosis of Chagas disease.

This paper develops a synthetic disaccharide biomarker for Chagas disease serodiagnosis, showing high sensitivity and accuracy in distinguishing infected from healthy sera.

Decoding efficacy and resistance space at a drug binding site

The paper uses MOT-library profiling to assess all possible mutations at a drug binding site and predicts resistance mechanisms through computational modeling.

ZNK1 senses zinc and degrades zinc transporter mRNA in trypanosomes

This paper identifies ZNK1 as a zinc-sensing RNA-binding protein in trypanosomes that degrades ZIP3 mRNA in response to zinc availability.

Experimental Trypanosoma cruzi infection cured with a mechanistically distinct drug combination

The paper shows that a combination of GNF6702 and benznidazole can cure experimental chronic Chagas disease with shorter treatment duration and fewer side effects.

Thermo-sensing and argonaute-dependent transcriptome remodelling in trypanosomes

This study identifies temperature-sensitive mRNA structures and shows that argonaute-dependent transcriptome remodelling mediates thermo-sensing in trypanosomes.

Transcriptome analysis of circulating microRNAs associated with Chagas disease susceptibility and chronic Chagas cardiomyopathy severity

This study identifies differentially expressed microRNAs in Chagas disease patients and links them to disease susceptibility and severity of chronic Chagas cardiomyopathy.

Structural Basis of Glycolytic Control in Trypanosoma cruzi: Insights from Enolase and PGI

The paper reports high-resolution structures of T. cruzi glycolytic enzymes and identifies potential inhibitors through structural analysis and virtual screening.

Triatomine host preference in an experimental cafeteria: the impact of Trypanosoma cruzi infections

This study investigates whether Trypanosoma cruzi-infected triatomine insects prefer mammalian hosts over non-mammalian ones, finding that infected T. gerstaeckeri show no host preference while uninfected ones prefer dogs.

Maternal Trypanosoma cruzi infection is associated with significant placental remodeling regardless of vertical transmission

This study shows that maternal Trypanosoma cruzi infection causes significant placental remodeling and systemic changes, regardless of whether the infection is transmitted to the fetus, and identifies potential biomarkers for prenatal monitoring.

Metagenomic enables the first detection of Trypanosoma sp. in Streblidae (Diptera: Hippoboscoidea) parasitizing bats in Sao Paulo, Brazil.

The study detects Trypanosoma sp. in Streblidae flies parasitizing bats using shotgun metagenomics, expanding knowledge of Neobat 4 clade distribution.

Small Heat Shock Proteins have a Paramount Role inTrypanosoma cruzi Infection Impacting Intestinal Homeostasis of an Insect Vector of Chagas Disease

This study shows that small heat shock proteins (sHSPs) in Rhodnius prolixus are critical for gut homeostasis and parasite control during Trypanosoma cruzi infection.