Adenosine and Purinergic Signaling

Shared mechanisms of dopamine and ATP transmission in the nucleus accumbens

This study examines ATP and dopamine co-release in the nucleus accumbens using FSCV, showing their coupled release and shared regulatory mechanisms.

A feed-forward loop between niche adenosine and Gzmk⁺ CD8 T cells propagates systemic inflammaging

This study identifies an adenosine-Gzmk axis that drives T cell aging and systemic inflammaging, showing that targeting it can reverse aging phenotypes and extend lifespan in mice.

Platelet-specific P2Y1 receptor deficient mice have suppressed leukocyte recruitment in response to lipopolysaccharide.

This study creates a platelet-specific P2Y1 receptor knockout mouse model and shows it suppresses leukocyte recruitment during inflammation.

Optimized Methods for Measuring Extracellular ATP from Human Airway Epithelial Cells and Bronchoalveolar Lavage Fluid

This paper introduces an optimized method to measure extracellular ATP in human airway samples, showing improved stability and specificity for eATP quantification.

Inhibition of (interstitial) P2Y6 receptors attenuates renal fibrosis progression

This study shows that inhibiting P2Y6 receptors reduces renal fibrosis in mice by preventing fibroblast activation and migration.

Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during BV2 microglial activation

The paper introduces X7-uP, a chemical labeling reagent for P2X7 receptors, enabling super-resolution imaging of their nanoscale redistribution in BV2 microglial cells during activation.

Cryo-EM reveals the structural basis of subtype-specific, noncompetitive inhibition of the human P2X3 receptor

The paper reports the cryo-EM structure of the human P2X3 receptor complexed with sivopixant, revealing subtype-specific allosteric inhibition mechanisms and structural basis for improved selectivity.

ATP increases murine neuroblastoma cell size through a PANX1- and macropinocytosis-dependent mechanism

This study shows ATP increases murine neuroblastoma cell size via PANX1-dependent macropinocytosis, with PANX1 internalization and lipid interactions involved.

The extracellular ATP/P2X7R signaling axis drives early neuroinflammation and neuronal hyperexcitability in an Alzheimer's disease mouse model

Neuroprotective action of agonists and modulators of A 1 adenosine receptors upon hyperexcitation: mechanism of the antiepileptic activity and role of neuron-glial interaction

This study investigates the antiepileptic and neuroprotective effects of A1 adenosine receptor agonists and modulators in various models of hyperexcitation, showing their ability to suppress seizures and improve survival.

Jasmonate Primes Plant Responses to Extracellular ATP through Purinoceptor P2K1

This study shows jasmonate primes plant responses to extracellular ATP via P2K1, enhancing calcium elevation and transcriptional reprogramming in a JA signaling-dependent manner.

Molecular basis of ligand binding and receptor activation at the human A3 adenosine receptor

The paper presents cryo-EM structures of the human A3 adenosine receptor in three functional states, revealing a unique activation mechanism and a cryptic binding pocket.

A2AR-mediated inhibition of B cell maturation: a novel mechanism of immune suppression in cancer

This study shows that A2AR inhibition enhances B cell maturation into antibody-secreting cells in the tumor microenvironment, reversing immune suppression.

Bayesian inference of functional asymmetry in a ligand-gated ion channel

The paper uses Bayesian inference and molecular dynamics to show asymmetric coupling in P2X2 receptors, explaining negative cooperativity and the flip state.

P2Y2 receptors are essential for hepatic clearance ofuropathogenic E. coli in a murine sepsis model

This study shows that P2Y2 receptors are essential for hepatic clearance of uropathogenic E. coli, leading to improved survival in a murine sepsis model.

Spatiotemporal dynamics of CD73 in mouse retina under physiological conditions

This study examines the spatiotemporal expression of CD73 in the mouse retina across development and adulthood, revealing its role in retinal function and adenosine signaling.

Sensing of extracellular ATP via P2RX7 drives lung tumor growth through regulatory T cell suppressive function

This study identifies P2RX7 as a key regulator of Treg suppressive function in lung tumors, showing that targeting P2RX7 could improve lung cancer treatment by reducing Treg-mediated immune suppression.

On-cell Saturation Transfer Difference (STD) NMR on ion channels: characterizing negative allosteric modulator binding interactions of P2X7.

This paper demonstrates on-cell STD NMR for studying P2X7 receptor-ligand interactions, providing binding epitope maps and 3D models for two antagonists.

Adenosine 2B receptor signaling impairs vaccine-mediated protection against pneumococcal infection in young hosts by blunting neutrophil killing of antibody opsonized bacteria

This study shows that adenosine 2B receptor signaling impairs neutrophil killing of antibody-opsonized pneumococci in young vaccinated mice, reducing vaccine efficacy, but not in aged mice.

Characterization of AZ12464237 as a high affinity, non-nucleotide antagonist radioligand for the P2Y12 receptor

This paper characterizes [3H]AZ12464237 as a high-affinity radioligand for the P2Y12 receptor, showing selectivity and utility in in vitro studies.

Structural basis of ATP release and ion selectivity in Pannexin1 channels

This study identifies phosphorylation as a switch that stabilizes Pannexin1 in an ATP-releasing conformation, important for developing disease modulators.

UB-MBX-46 is a potent and selective antagonist of the human P2X7 receptor developed by structure-based drug design

The paper identifies molecular features of the human P2X7 receptor using cryo-EM structures and designs a potent, selective antagonist, UB-MBX-46, with picomolar potency and high selectivity.

The effect of oxidative stress on the Adenosine A2a Receptor structure, activity and signalling

This study investigates how oxidative stress affects the structure, activity, and signaling of the Adenosine A2a Receptor (A2aR), finding no structural impact but increased cAMP levels in response to agonists under oxidative conditions.

ATP and glutamate coordinate contractions in the freshwater sponge Ephydatia muelleri

This study shows ATP and glutamate work together in freshwater sponges to trigger whole-body contractions, suggesting ancient signaling mechanisms predating nervous systems.

Discovery of the first-efficacious A2AR negative allosteric modulators for high adenosine cancer immunotherapies

The paper identifies potent A2AR negative allosteric modulators through high-throughput screening and SAR analysis, showing their efficacy in high-adenosine conditions for safer cancer immunotherapies.

Supracellular contractility in Xenopus laevis embryonic epithelia regulated by extracellular nucleotides and the purinergic G-protein coupled receptor P2Y2.

The paper identifies extracellular nucleotides ATP and UTP as drivers of epithelial contractility in Xenopus embryos, mediated by the P2Y2 receptor.

Human P2X4 receptor gating is modulated by a stable cytoplasmic cap and a unique allosteric pocket

The paper reports cryo-EM structures of human P2X4 receptor in different states, revealing the cytoplasmic cap forms before agonist binding and is stabilized by lipids, with unique allosteric features for modulator development.

Endogenously expressed P2X5 includes a proportion of isoforms or transcript variants sensitive to extracellular ATP

The study identifies ATP-sensitive P2X5 isoforms and transcript variants in multiple species, challenging the assumption of their ATP-insensitivity.

Ectonucleotidases and Purinergic Receptors in Mouse Prostate Gland

This study identifies differential expression and functional roles of ectonucleotidases and purinergic receptors in mouse prostate cells, revealing their involvement in smooth muscle contraction and potential relevance to prostate diseases.

Novel naphthyridones targeting Pannexin 1 for colitis management

The paper identifies a novel naphthyridone inhibitor of PANX1 with reduced off-target effects, showing efficacy in a mouse model of IBD.