Adenosine and Purinergic Signaling

A Second Drug Binding Site in P2X3

This study identifies a second drug-binding site in P2X3 receptors and shows camlipixant acts as an allosteric inhibitor, providing insights into its selectivity and mechanism of action.

The C-terminal activating domain promotes Panx1 channel opening

This paper identifies the C-terminal activating domain (CAD) as essential for Panx1 channel opening, showing how its cleavage repositions the N-terminus to reduce anion accumulation and promote ion permeability.

Selective regulation of IFN-γ and IL-4 co-producing unconventional T cells by purinergic signalling

This study shows that purinergic signaling via ARTC2 and P2RX7 regulates the survival and function of unconventional T cells, affecting IFN-γ and IL-4 production.

Modulation of the ATP-adenosine signaling axis combined with radiotherapy facilitates anti-cancer immunity in brain metastasis

This study shows that blocking ATP-to-adenosine enzymes and combining with radiotherapy improves anti-cancer immunity in brain metastases by altering immune cell populations and tumor inflammation.

Novel carboxy-terminal Pannexin 1 isoform is expressed in mouse melanoma cells and interacts with the full-length protein

Insights from Molecular Docking and Dynamics Simulations of P2RX7-????Syn Complex

This paper uses molecular docking and dynamics simulations to study the interaction between P2RX7 and alpha-synuclein, identifying binding sites and structural changes involved in neuronal damage.

Elucidating Interactome Dynamics of the A2A Adenosine Receptor

The paper investigates the effects of PDRN on ADORA2A-interacting proteins through proteomic analysis and network modeling, identifying novel interactions and potential sites involved in neuronal differentiation.

Retinal microglial cells increase expression and release of IL-1β when exposed to ATP

This study shows that retinal microglial cells increase IL-1β expression and release when exposed to ATP via the P2X7 receptor, with effects distinct from astrocytes.

Norepinephrine changes behavioral state via astroglial purinergic signaling

The paper shows norepinephrine induces astroglial purinergic signaling that suppresses behavior through adenosine receptor activation in zebrafish.

Adenosinergic modulation of layer 6 microcircuitry in the medial prefrontal cortex is specific to presynaptic cell type

The paper investigates adenosinergic modulation in layer 6 of the medial prefrontal cortex in rats, revealing differential A1AR expression and synaptic sensitivity between two excitatory neuron subtypes.

Identification of a carbohydrate-recognition motif of purinergic receptors

The paper identifies a conserved carbohydrate-recognition motif in purinergic receptors and demonstrates its role in activating GPCRs through sugar nucleotide binding.

Spontaneous and multifaceted ATP release from astrocytes at the scale of hundreds of synapses

The paper shows astrocytes release ATP spontaneously and non-vesicularly, affecting hundreds of synapses without requiring calcium.

A GRAB sensor reveals activity-dependent non-vesicular somatodendritic adenosine release

The paper develops a genetically encoded adenosine sensor to show activity-dependent non-vesicular adenosine release from somatodendritic regions via calcium influx and equilibrative transporters.

Ion permeation pathway within the internal pore of P2X receptor channels

The paper identifies a critical residue in P2X receptor channels that influences ion permeability through lateral fenestrations.

Species-dependent actions of the Gαob selective adenosine A1 receptor agonist BnOCPA

The paper compares the effects of BnOCPA, an A1R agonist, in rat and mouse hippocampal neurons, finding differences in its functional selectivity between pre- and postsynaptic receptors.

Adenosine A1R/A3R Agonist AST-004 Reduces Brain Infarction in Mouse and Rat Models of Acute Ischemic Stroke

This study shows AST-004, an A1R/A3R agonist, reduces brain infarction in mouse and rat stroke models, with efficacy dependent on A3R signaling and astrocyte mitochondrial metabolism.

Crosstalk between AQP4-dependent ATP/Adenosine release and dopamine neurotransmission in depressive behavior during cocaine withdrawal

The paper investigates how AQP4-dependent adenosine release interacts with dopamine neurotransmission during cocaine withdrawal, showing AQP4's role in modulating depressive behavior.

Mu opioid receptors acutely regulate adenosine signaling in a thalamo-striatal circuit.

This study shows that opioid receptors inhibit adenosine signaling in the striatum by reducing cAMP, specifically affecting D1R-positive medium spiny neurons.

Dopamine release in nucleus accumbens is under tonic inhibition by adenosine A1 receptors regulated by astrocytic ENT1 and dysregulated by ethanol

This study investigates how adenosine A1 receptors inhibit dopamine release in the nucleus accumbens, showing that astrocytic ENT1 regulates this inhibition and that ethanol disrupts it.

An ultrasensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo

The paper develops an ultrasensitive GRABATP1.0 sensor to detect extracellular ATP in real time, demonstrating its use in vitro and in vivo across multiple conditions.

Deletion of CD73 increases exercise power in mice

This study shows that deleting CD73 in mice increases critical power and maximum RER during exercise, indicating improved physical performance.

Functional expression of the ATP-gated P2X7 receptor in human iPSC-derived neurons and astrocytes

This study demonstrates functional P2X7R expression in human iPSC-derived neurons and astrocytes using calcium imaging and immunostaining.

Adenosine receptor and its downstream targets, mod(mdg4) and Hsp70, work as a signaling pathway modulating cytotoxic damage in Drosophila

This study investigates the role of adenosine signaling in Drosophila, showing that mod(mdg4) and Hsp70 mediate its protective effects against cytotoxic damage.

ATP signaling in the integrative neural center of Aplysia californica

This study characterizes P2X receptors in Aplysia californica, showing their expression in chemosensory systems and an integrative neurosecretory center, highlighting evolutionary conservation of purinergic signaling.

P2X-GCaMPs as versatile tools for imaging extracellular ATP signaling

The paper develops P2X-GCaMP biosensors to detect extracellular ATP with high sensitivity and tracks ATP release in response to neuronal depolarization and hypotonicity.

P2X receptors in Aplysia californica: Chemosensory systems, bio-energetic and development

The paper characterizes P2X receptors in Aplysia californica, showing their role in chemosensory systems and bioenergetic centers, and demonstrates evolutionary conservation of purinergic signaling.

Sleep deprivation selectively down-regulates astrocytic 5-HT2B receptors and triggers depressive-like behaviors via stimulating P2X7 receptors

This study shows chronic sleep deprivation reduces astrocytic 5-HT2B receptors via P2X7 receptors, leading to depressive-like behaviors through increased arachidonic acid and PGE2 release.

The Cryo-EM Structure of a Pannexin 1 Channel Reveals an Extracellular Gating Mechanism

The paper presents the cryo-EM structure of a pannexin 1 channel, revealing an extracellular gating mechanism and identifying key residues involved in ion selectivity and channel inhibition.

Simulation of P2X-mediated calcium signaling in microglia

The paper develops a computational model to simulate P2X-mediated calcium signaling in microglia and quantifies TNF production under various ATP stimulation conditions.

Patterned expression of Pannexin 1 channels in the adult cerebellar Purkinje cells

The paper describes the patterned expression of Pannexin 1 channels in specific cell types of the adult mouse cerebellum, correlating with the zebrin II distribution.