F. Li

Rapid quantification of polyhydroxyalkanoates accumulated in living cells based on green fluorescence protein labeled phasin: The qPHA method

The paper develops a method to rapidly quantify PHA in living cells using GFP-labeled phasins, achieving high accuracy with R-squares over 0.9.

A Genome-wide CRISPR Screen Identifies WDFY3 as a Novel Regulator of Macrophage Efferocytosis

This study identifies WDFY3 as a novel regulator of macrophage efferocytosis through a genome-wide CRISPR screen, revealing its role in actin depolymerization and lysosomal acidification.

Genomic insights into the phylogeny and biomass-degrading enzymes of rumen ciliates

The paper presents 52 high-quality genomes of rumen ciliates, reclassifying them into 19 species and identifying their biomass-degrading enzymes.

VRK1 is a Paralog Synthetic Lethal Target in VRK2-methylated Glioblastoma

This study identifies VRK1 as a synthetic lethal target in VRK2-methylated glioblastoma by showing that VRK1 inhibition leads to tumor growth inhibition in xenograft models.

3-Phosphoinositide-dependent kinase 1 drives acquired resistance to osimertinib

This study identifies PDK1 as a driver of osimertinib resistance in NSCLC, showing its inhibition sensitizes resistant cells and correlates with disease progression.

A potent human monoclonal antibody with pan-neutralizing activities directly dislocates S trimer of SARS-CoV-2 through binding both up and down forms of RBD

This study identifies a potent human monoclonal antibody, 35B5, that neutralizes SARS-CoV-2 and its variants by targeting a conserved epitope on the RBD, offering a potential therapeutic and vaccine design strategy.

Simulation-based inference of evolutionary parameters from adaptation dynamics using neural networks

The paper estimates beneficial mutation rates and fitness effects using simulation-based Bayesian inference on CNV dynamics in yeast.

Unidirectional Single-File Transport of Full-Length Proteins Through a Nanopore

This paper demonstrates a method for unidirectional transport of full-length proteins through nanopores using guanidinium chloride and machine learning to identify protein orientation and identity.

Construction of a solid Cox model for AML patients based on multiomics bioinformatic analysis

This study constructs a prognostic model for AML using multiomics data to identify immune-related biomarkers and classify patients into subtypes with different survival outcomes.

Molecular basis for the R-type anion channel QUAC1 activity in guard cells

This study determines the cryo-EM structure of QUAC1, an R-type anion channel, revealing its dimeric architecture and mechanism of malate-mediated activation.

The cause of on-target point mutations generated by CRISPR-Cas9 treatment in the yeast Xanthophyllomyces dendrorhous

This study identifies on-target point mutations caused by CRISPR-Cas9 in yeast, showing sequence dependence and involvement of NHEJ components.

Opposite effects of nutrient enrichment and herbivory by an alien snail on growth of an invasive macrophyte and native macrophytes

This study examines how nutrient enrichment and herbivory by an invasive snail interactively affect growth of an invasive macrophyte and native macrophytes in freshwater mesocosms.

Ribosomal protein L23 drives the metastasis of hepatocellular carcinoma via upregulating MMP9

This study identifies ribosomal protein L23 as a driver of hepatocellular carcinoma metastasis by upregulating MMP9 expression and promoting tumor growth and metastasis in vivo.

Amplification-free detection of viral RNA by super resolution imaging-based CRISPR/Cas13a System

This paper develops a CRISPR/Cas13a system combined with super resolution imaging for direct, amplification-free viral RNA detection with improved sensitivity.

Allosteric Inhibition of a Vesicular Glutamate Transporter by an Isoform-Specific Antibody

This study develops isoform-specific antibodies against VGLUT2 to investigate allosteric inhibition mechanisms and their physiological roles.

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell wall acetylation

The study identifies a secondary metabolite produced by L. reuteri that mediates intraspecies antagonism, with resistance linked to cell wall acetylation.

Engineered AIM-based Selective Autophagy to Degrade Proteins and Organelles

The paper introduces a method to degrade specific proteins and organelles using engineered autophagy receptors, demonstrating its effectiveness in plant and transient expression systems.

Long-term culture of fetal monocyte precursors in vitro allowing the generation of bona fide alveolar macrophages in vivo

This study demonstrates the in vitro generation of alveolar macrophage-like cells from fetal liver monocytes using GM-CSF, which can engraft in vivo and protect against respiratory infections.

Structural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing

This study reveals the structural basis of SETD2 methyltransferase interaction with hnRNP L paralogs, showing how a leucine pair in SETD2 interacts with hnRNP L RRM2 and affects splicing regulation.

CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.

The paper uses cryo-EM and AlphaFold2 to determine the structure of SARS-CoV-2 Nsp2, revealing its zinc-binding site and potential interactions with host proteins.

Platelet HMGB1 in Platelet-Rich Plasma Promotes Tendon Wound Healing

This study shows that platelet HMGB1 in PRP enhances tendon healing in mice, with PRP from GFP mice promoting faster healing and better tissue organization compared to PRP from HMGB1 KO mice.

Structural basis for HCMV Pentamer recognition by antibodies and neuropilin 2

This study determines the cryo-EM structure of HCMV Pentamer bound to neuropilin 2 and neutralizing antibodies, revealing molecular interactions and epitopes involved in viral tropism and neutralization.

Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius.

This study identifies a feline commensal bacterium, S. felis, that inhibits drug-resistant S. pseudintermedius through antimicrobial peptides, showing potential for bacteriotherapy against skin infections.

Pharmacological targeting of a PWWP domain demonstrates cooperative control of NSD2 localization

The paper develops a chemical probe, UNC6934, that targets the PWWP1 domain of NSD2, revealing cooperative control of NSD2 localization through chromatin reader domains.

Geminiviral genomes encode additional proteins with specific subcellular localizations and virulence function

This study identifies additional ORFs in geminiviral genomes that encode small proteins with specific subcellular localizations and virulence functions.

Response of whitefly to the wild tomato Solanum habrochaites

This study examines how the wild tomato Solanum habrochaites affects the survival, fecundity, and gene expression of the whitefly Bemisia tabaci, revealing stress gene induction and potential for pest control.

Probing the SAM Binding Site of SARS-CoV-2 nsp14 in vitro Using SAM Competitive Inhibitors Guides Developing Selective bi-substrate Inhibitors

This paper identifies SAM competitive inhibitors of SARS-CoV-2 nsp14 methyltransferase and characterizes their mechanism, guiding the development of selective bi-substrate inhibitors.

A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex

The paper develops a radioactivity-based high-throughput screening assay for the SARS-CoV-2 nsp10-nsp16 complex and optimizes it for HTS with a Z'-factor of 0.83.

Production of SARS-CoV-2 virus-like particles in insect cells

This study produces SARS-CoV-2 virus-like particles in insect cells for vaccine development and research.

Identification of candidate susceptibility genes to Puccinia graminis f. sp. tritici in wheat

The paper identifies candidate susceptibility genes to wheat stem rust disease by analyzing gene expression changes in susceptible and resistant wheat genotypes and a non-host plant.