Microtubule and mitosis dynamics

The kinetochore corona orchestrates chromosome congression through transient microtubule interactions

This paper uses live-cell microscopy and computational modeling to show that chromosome congression is driven by stochastic, transient microtubule interactions at the kinetochore corona.

Proximity labeling reveals cell cycle-specific NEK2 interactions and a regulatory axis controlling NUSAP1 stability

The paper uses proximity labeling and mass spectrometry to map NEK2 interactions across the cell cycle, revealing a NEK2-NUSAP1 degradation pathway.

N-terminal phosphorylation inhibits Arabidopsis katanin and affects vegetative and reproductive development in opposite ways

This study shows that phosphorylation of Arabidopsis katanin regulates microtubule severing, affecting vegetative growth and male fertility in opposite ways.

Targeted therapy-induced chromosomal instability dictates mitotic dependency on Aurora Kinase A

This study shows that KRASG12C inhibition induces chromosomal instability (CIN) in NSCLC cells, which synergizes with Aurora Kinase A inhibition to enhance therapeutic efficacy.

Mechanical force locally damages, remodels and stabilizes the lattice of spindle microtubules

This study shows that mechanical force causes local damage and remodeling of spindle microtubules, leading to stabilization under force.

Explainable Machine Learning Identifies Dosage Compensation Factors in Aneuploid Human Cancer Cells

This study uses explainable machine learning to identify factors that buffer protein levels in aneuploid cancer cells, finding key predictors and implications for therapy.

Bayesian data driven modelling of kinetochore dynamics: space-time organisation of the human metaphase plate

The paper uses Bayesian inference to analyze kinetochore dynamics in human cells, identifying spatial and temporal organization patterns during mitosis.

A nucleotide code governs Lis1's ability to relieve dynein autoinhibition

The paper identifies nucleotide codes that regulate dynein-Lis1 binding and reveal how Lis1 relieves dynein autoinhibition through structural changes.

Cell and nuclear size are associated with chromosomal instability and tumorigenicity in cancer cells that undergo whole genome doubling

The paper shows that cell and nuclear size in tetraploid cancer cells correlate with chromosomal instability and tumorigenicity, with smaller cells having better fitness and poorer patient survival in WGD-positive cancers.

Loss of T73T75 phosphorylation on PP2A-B56Par1 advances mitotic entry and reduces S. pombe cell size

The paper identifies that phosphorylation of T73T75 on PP2A-B56Par1 by CDK1-Cyclin B delays mitotic entry and increases cell size in S. pombe.

Chromosomal instability increases radiation sensitivity

The paper shows that higher chromosomal instability (CIN) in cancer cells and tumors increases sensitivity to radiation, both directly and through drug-induced CIN mechanisms.

GMCL1 Controls 53BP1 Stability and Modulates Taxane Sensitivity

This study identifies GMCL1 as a negative regulator of the mitotic stopwatch pathway, showing it promotes 53BP1 degradation and reduces paclitaxel sensitivity in cancer cells.

Structural characterization of a minimal KLC2/Nup358/BicD2 complex

The paper characterizes the structure of a KLC2/Nup358/BicD2 complex using cryo-EM and SAXS, showing changes in stoichiometry and structure upon BicD2 binding.

SSNA1 mechanically reinforces the damaged microtubule lattice

This study shows SSNA1 strengthens microtubules by increasing rigidity and resistance to breakage while blocking tubulin-mediated repair at damage sites.

Recognizing dUTPase as a mitotic factor essential for early embryonic development

This study identifies dUTPase as a mitotic regulator essential for early embryonic development, showing its role in centrosome integrity and spindle dynamics beyond its known nucleotide sanitization function.

Molecular structure of the ESCRT III-based archaeal CdvAB cell division machinery

The paper presents structural studies of the archaeal CdvAB cell division machinery, showing CdvA forms helical filaments and CdvB adopts ESCRT-III-like folds, revealing membrane engagement mechanisms.

Distinct p21 dynamics drive alternative routes to whole-genome duplication through a common CDK4/6-dependent polyploid G0 state

The paper identifies two distinct p21-driven pathways to whole-genome duplication in breast cancer cells, both converging on a CDK4/6-dependent polyploid G0 state, and shows that CDK4/6 inhibitors can block WGD and enhance therapy efficacy.

Annexin A2 and lamin B join membrane recycling compartments for the assembly of biomolecular condensates operating in mitotic partitioning

This study identifies Annexin A2 and lamin B-containing biomolecular condensates in mitotic cells that facilitate membrane recycling and partitioning of endomembranes during cell division.

CCAN forms the spherical shell-like structure in interphase kinetochores

The paper shows that CCAN forms a spherical shell-like structure in interphase kinetochores using in silico and in vivo analyses.

Multiple clustered centrosomes in antigen-presenting cells foster T cell activation without MTOC polarization

This study shows that multiple clustered centrosomes in antigen-presenting cells enhance T cell activation by positioning near the cell center, unlike T cells where centrosomes polarize toward the immune synapse.

Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons

This study directly labels microtubule turnover in developing neurons, revealing in-lattice repair and stabilization patterns during axon formation.

Tubulin autoregulation controls the biosynthesis of γ-tubulin to ensure mitotic fidelity

The paper shows that γ-tubulin is regulated by the same autoregulatory pathway as α- and β-tubulin, ensuring proper microtubule nucleation and mitotic fidelity.

Restoring Shugoshin 1 reduces chromosome errors in human eggs

This study shows that restoring Shugoshin 1 reduces chromosome errors in aged human eggs by preserving cohesion.

Kinetochore Homeostasis is Maintained by Coordinated Chromatin Stabilization and Soluble Buffering

The paper shows that CENP-T homeostasis is maintained by two distinct pathways involving chromatin stabilization and soluble buffering.

Dual pathways via CENP-C and Mis18C recruit HJURP for CENP-A deposition into vertebrate centromeres

This study identifies two recruitment pathways for HJURP to centromeres via CENP-C and Mis18C, essential for CENP-A deposition and centromere maintenance in chicken cells.

Distinct pools of CENP-A and CENP-C support unique phases of centromere assembly in spermatogenesis.

The paper shows that CENP-C and CID have distinct functional pools during Drosophila spermatogenesis, with different roles in centromere assembly and kinetochore function.

Tau condensation on DNA and localization on centromeres: A potential link to cell division

This study shows tau protein binds DNA, forms condensates, and localizes to centromeres, suggesting a new role in mitosis and cell division.

Structural transitions in kinesin minus-end directed microtubule motility

This paper reports high-resolution structures of kinesin-14 motor proteins during different mechanochemical states, revealing structural transitions that drive force generation and the power stroke.

The Structural Basis of alpha/beta-tubulin Assembly and Disassembly by Tubulin Cofactors

This study determines the cryo-EM structures of TBC-DE and TBC-DEG assemblies to elucidate their mechanisms in regulating β-tubulin assembly and disassembly.

Linking Kinetochore Attachment to Checkpoint Control: The Role of Aurora B in BubR1 Acetylation

This paper identifies Aurora B-mediated phosphorylation and acetylation of BubR1 as critical for SAC signaling, linking kinetochore attachment to checkpoint activity and offering therapeutic targets for chromosome instability cancers.