Autophagy in Disease and Therapy

Isolation of functional lysosomes from skeletal muscle

This paper describes a method to isolate functional lysosomes from mouse skeletal muscle tissue, preserving their structure and function, and demonstrates reduced lysosomal activity in aging muscle.

Chaperone AIP Couples mTORC1 Activation and Catabolic Metabolism During Neonatal Development

The paper identifies AIP as a regulator that links mTORC1 activation with catabolic metabolism during neonatal development, ensuring metabolic adaptation and survival.

Phase separation promotes Atg8 lipidation for autophagy progression

This study shows that phase separation of the Atg1 complex forms the PAS, which facilitates Atg8 lipidation and autophagosome formation in yeast.

Endocytic clathrin-coated vesicles are targeted for selective autophagy during osmotic stress.

The paper identifies a mechanism in plants where clathrin-coated vesicles are selectively degraded via autophagy during osmotic stress to maintain membrane tension and cell integrity.

BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM

The paper identifies BLTP3A as a protein associated with late endocytic membranes and shows it interacts with the CASM pathway to respond to lysosome damage.

Mitochondria-Lysosome Crosstalk Shapes Metabolic Transition in Neonatal Enterocytes

This study identifies the mitochondria-lysosome axis as critical for maintaining the function of neonatal lysosome-rich enterocytes and preventing premature differentiation.

Lysosomal RNA profiling reveals targeting of specific types of RNAs for degradation

This study identifies lysosomal RNases and shows specific RNA types are targeted for degradation via autophagy-dependent mechanisms.

Functional autophagy gene set signature and state classification reveal a link between autophagy induction, lysosomal activity, and poor prognosis in glioblastoma

The paper develops a functional autophagy gene set signature to classify autophagy states and links autophagy induction and lysosomal activity to poor prognosis in glioblastoma.

Targeting a novel chloroquine derivative to lysosomes induces massive and irreversible damage to lysosomes and suppresses autophagosomes and lysosomes assembly in cancer

This paper develops a novel chloroquine derivative, Lysostilbene-4, which irreversibly damages lysosomes and suppresses their recovery in pancreatic cancer cells.

TECPR2 maintains mitochondrial homeostasis in neurodegeneration

This study shows that TECPR2 loss causes mitochondrial dysfunction and mitophagy impairment in HSAN9, which can be mitigated by ISR suppression.

Targeted degradation of SARS-CoV-2 via the autophagy-lysosome system using chemical mimetics of the N-degron pathway

This study shows that chemical mimetics of the N-degron pathway, called ATLs, can degrade SARS-CoV-2 via autophagy-lysosome system in cells and animals.

Peri-mitochondrial actin filaments inhibit Parkin assembly via disruption of ER-mitochondrial contact

The paper shows that peri-mitochondrial actin filaments (ADA) inhibit Parkin assembly by disrupting ER-mitochondrial contacts, delaying mitophagy after acute damage and blocking it during chronic dysfunction.

Processing bodies promote lysosomal quality control and cell survival during recovery from lysosomal damage

This study identifies processing bodies (PBs) as critical for lysosomal quality control and cell survival during recovery from lysosomal damage, distinguishing their role from the acute damage response.

A singular lipid composition supports the progression of plant autophagy

This study identifies a unique lipid composition in plant autophagic membranes, showing phosphatidylglycerol's functional role in autophagy activity.

Unc-51-like Kinase 1 (ULK1) Regulates Bacterial Ubiquitylation and p62 Recruitment during Xenophagic Clearance of Listeria monocytogenes

This study identifies ULK1's role in bacterial ubiquitylation and p62 recruitment during xenophagy, showing its importance in autophagic clearance of Listeria monocytogenes.

Soma-centered control of synaptic autophagy by Rab39-regulated anterograde trafficking of Atg9

This study identifies Rab39 as a regulator of autophagy at presynaptic terminals in Drosophila, showing its role in trafficking Atg9 vesicles and its link to neurodegeneration.

Nonuple atg8 mutant provides genetic evidence for functional specialization of ATG8 isoforms in Arabidopsis thaliana

The paper shows that different ATG8 isoforms in Arabidopsis have specialized functions in autophagy, as demonstrated by a mutant lacking all nine isoforms and complementation experiments.

PEX14 functions as a molecular link between optineurin and pexophagy in human cells

This study identifies PEX14 as a molecular link between optineurin and pexophagy, elucidating its role in recruiting autophagic machinery for peroxisome degradation.

Transcriptional Dynamics Uncover the Role of BNIP3 in Mitophagy during Muscle Remodeling in Drosophila

The paper uses time-course RNA-seq to study muscle remodeling in Drosophila, identifying BNIP3-mediated mitophagy as critical for mitochondrial degradation during this process.

Autophagic degradation of EIN3 ensures developmental plasticity and recovery from environmental stress in Arabidopsis

This study shows autophagy regulates EIN3 stability, affecting Arabidopsis development and stress responses.

Most Autophagic Cell Death Studies Lack Evidence of Causality

This study evaluates 104 2022 papers claiming to show autophagic cell death, finding most lack causality evidence and suffer from methodological and terminological issues.

Ancestral P-body proteins rewired for autophagic recyclingin the early land plant Marchantia polymorpha

This study identifies EDC4 and DCP1 as autophagy receptors for P-body turnover in Marchantia polymorpha, revealing lineage-specific adaptations and potential therapeutic applications.

Mitochondrial damage triggers concerted degradation of negative regulators of neuronal autophagy

The paper identifies a novel stress response pathway, MitoSR, triggered by mitochondrial damage that enhances mitophagy by degrading negative regulators in neurons.

Autophagy maintains HEV identity and function during inflammation

This study shows autophagy is essential for maintaining HEV function and expansion during inflammation, ensuring proper PNAd production and lymphocyte homing.

ER-phagy Receptors Intrinsically Disordered Modules Drive ER Fragmentation and ER-phagy

This study shows that intrinsically disordered regions (IDRs) of ER-phagy receptors drive ER fragmentation, not their membrane-remodeling domains.

Treatment of age-related decreases in GTP levels restores endocytosis and autophagy

The paper shows that restoring GTP levels in aged neurons improves endocytosis and autophagy, reducing Aβ aggregates and oxidative damage.

GENETIC ANALYSIS OF ARABIDOPSIS AUTOPHAGY-RELATED 8 FAMILY PROTEINS REVEALS THEIR REDUNDANT AND REGULATORY ROLES IN PLANT AUTOPHAGY

This study uses CRISPR/Cas9 to knockout Arabidopsis ATG8 genes, revealing their redundant and regulatory roles in autophagy, with Clade I and II ATG8s showing overlapping functions and interactions with RABG3 proteins.

Impact of O-GlcNAcylation elevation on mitophagy and glia in the dentate gyrus

This study shows that elevated O-GlcNAcylation reduces mitophagy in astrocytes and alters glia homeostasis in the dentate gyrus.

A dual component system instructs membrane hydrolysis during the final stages of plant autophagy

This study identifies two phospholipases, LCAT3 and LCAT4, that work together to hydrolyze autophagic bodies in plant cells, completing the autophagy pathway.

Mammalian cells evacuate and shelter mitochondrial DNA from destruction following hypoxia response-induced mitophagy

The paper shows mammalian cells protect mitochondrial DNA during hypoxia-induced mitophagy by forming large mitochondria that shield mtDNA from degradation.