Autophagy in Disease and Therapy

In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment

Cryo-ET of Parkin-expressing U2OS cells shows oligomycin/antimycin depolarization moves some ATP synthase to the inner boundary membrane, abolishes matrix calcium granules, and favors closed prohibitin domes.

ATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains

This study shows ATXN3 helps restore lysosomal function after damage by targeting K48-K63-branched ubiquitin chains on regenerating lysosomes.

Investigating the relationship Between AMBRA1 and Cell Proliferation in Melanoma

This study examines AMBRA1's role in melanoma by analyzing transcriptomic changes from overexpression and knock-down, identifying novel cancer-related pathways and tumor suppressor functions.

ANKZF1 helps to eliminate the stress-damaged mitochondria by LC3-mediated mitophagy

This study identifies ANKZF1 as a key player in PINK1-Parkin-mediated mitophagy, showing its role in eliminating stress-damaged mitochondria through LC3 interaction.

Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

MEG3 Enhances Survival of Developing Human Neurons with CLCN4-Linked Autophagy Impairment

The paper identifies CLCN4 variants causing neuronal cell death and autophagy impairment, showing MEG3 restoration rescues these defects in human neurons.

Proteasome Inhibition Reduces NBR1 Protein Levels in mATG8-Deficient Cells Independently of Lysosomal Degradation and RAB27A

The paper shows that proteasome inhibition reduces NBR1 levels in mATG8-deficient cells through a non-lysosomal, RAB27A-independent pathway involving ubiquitin and HRS.

Liver CYP4A autophagic-lysosomal degradation (ALD): A major role for the autophagic receptor SQSTM1/p62 through an uncommon target interaction site

The paper identifies SQSTM1/p62 as a key autophagic receptor for CYP4A degradation, showing its role in preventing proinflammatory metabolite production and insoluble aggregates in liver cells.

Multi-site phosphorylation of BCL2L13 in a TBK1- and AMPK-dependent manner reveals new modes of mitophagy regulation

This study identifies phosphorylation sites in BCL2L13 regulated by AMPK and TBK1, revealing a novel mechanism for mitophagy regulation.

Structural Analyses of a GABARAP~ATG3 Conjugate Uncover a Novel Non-covalent Ubl-E2 Backside Interaction

The paper reports the crystal structure of a GABARAP~ATG3 conjugate, revealing a non-covalent interaction between GABARAP and the backside of ATG3's E2 catalytic center, which is important for PE conjugation.

Autophagic stress activates distinct compensatory secretory pathways in neurons

The paper shows that neurons with Parkinson's disease-associated LRRK2 mutations increase secretion of autophagosomes and exosomes to manage cellular waste and communication, potentially delaying disease onset but contributing to neuroinflammation.

ATG8i Autophagy activation is mediated by cytosolic Ca2+ under osmotic stress in Arabidopsis thaliana

This study shows osmotic stress and cytosolic Ca2+ regulate ATG8i autophagosome accumulation in Arabidopsis thaliana, with ATG8i involved in ER-phagy under stress.

The N-degron pathway governs autophagy to promote thermotolerance in Arabidopsis

The paper shows that ATG8a turnover via the Arg/N-degron pathway regulates autophagy and thermotolerance in Arabidopsis during heat stress.

Lysosome-like structures in the nucleolus mediate H2Aub degradation under glucose starvation

The paper identifies nucleolar lysosome-like structures that degrade H2Aub during glucose starvation, contributing to amino acid supply for energy and affecting cell growth.

Influence of ULK1 activity on the memory of ATG101 auto-activation dynamics

This study investigates how ULK1 activity influences ATG101 auto-activation dynamics, revealing a memory mechanism that accelerates autophagy complex formation.

Microbial Peptidoglycan Engages Autophagy Receptor P62 to Induce Protective Mitophagy in the Liver

This study identifies microbial peptidoglycan as a mitophagy inducer that protects against hepatocyte death and liver fibrosis by interacting with p62.

Muscle Type-Specific Modulation of Autophagy Signaling in Obesity: Effects of Caloric Restriction and Exercise

This study examines how obesity, caloric restriction, and exercise affect autophagy signaling in skeletal muscle, finding that CR+Ex reduces body mass and alters autophagy markers differently in red and white muscle.

Profiling Lysosomal and Mitochondrial Dysfunction in Neurodegenerative Diseases Using Human Fibroblasts for Translational Therapeutic Screening

The paper evaluates lysosomal and mitochondrial dysfunction in human fibroblasts from healthy donors and neurodegenerative disease patients, validating assays for high-throughput therapeutic screening.

Laminar Shear Stress Increases the Cytosolic Pool of PINK1 in Endothelial Cells and Enhances Mitophagic Sensitivity Toward Dysfunctional Mitochondria

This study shows laminar shear stress increases cytosolic PINK1 in endothelial cells, enhancing mitophagic sensitivity to dysfunctional mitochondria and improving cardiovascular health.

Lysosomal membrane permeabilization enhances the anticancer effects of RNA Polymerase I transcription inhibitors

This study shows that lysosomal membrane permeabilization enhances the effectiveness of RNA Polymerase I inhibitors against cancer cells.

The evolutionarily conserved PRP4K-CHMP4B/vps32 splicing circuit regulates autophagy

This study identifies a conserved PRP4K-CHMP4B/vps32 splicing circuit that regulates autophagy in both human cells and Dictyostelium discoideum.

The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy

This study identifies the p97-UBXD8 complex as a regulator of peroxisome abundance by suppressing pexophagy through ubiquitylation of PMP70.

Synergistic Control of Mitochondrial Dynamics and Function by ERAD and Autophagy in Brown Adipocytes

This study reveals a synergy between SEL1L-HRD1 ERAD and autophagy in maintaining mitochondrial structure and thermogenic capacity in brown adipocytes.

SARS-CoV-2 envelope protein induces LC3 lipidation via the V-ATPase-ATG16L1 axis

This study shows SARS-CoV-2 E protein induces LC3 lipidation through the V-ATPase-ATG16L1 axis, which supports viral replication rather than inhibiting it.

Cell-type specific autophagy in human leukocytes

The paper measures autophagic flux in 19 human blood cell sub-populations using flow cytometry, finding differences by cell type, sex, and age, with non-classical monocytes responding uniquely to amino acid withdrawal.

Balance between autophagy and apoptosis determines anoikis resistance: a mathematical model on cell fate

The paper develops a mathematical model to study how cancer cells balance autophagy and apoptosis to determine anoikis resistance.

Stereospecific rapid activation of Transcription Factor EB (TFEB) by Levacetylleucine (NALL)

This paper shows that NALL activates TFEB, enhancing lysosomal and autophagic function, which may explain its therapeutic effects in neurodegenerative diseases.

Autophagy across tissues of aging mice

This study examines autophagic flux changes with age and diet in mice, revealing sexual dimorphisms across tissues.

The developing retina undergoes mitochondrial remodeling via PINK1/PRKN-dependent mitophagy

The paper identifies two mitophagy peaks during retinal development, one PINK1-independent and one PINK1-dependent, linked to oxidative stress and RGC differentiation.

ATG9A and ARFIP2 cooperate to regulate PI4P levels for lysosomal repair

The paper shows ATG9A and ARFIP2 work together to regulate PI4P levels for lysosomal repair during damage and bacterial infection.