Glycosylation and Glycoproteins Research

Deep Learning Prediction of Intact N-/O-Glycopeptide Tandem Mass Spectra Enhances Glycoproteomics

This study introduces DeepGPO, a deep learning framework that accurately predicts N- and O-glycopeptide MS/MS spectra, enhancing glycoproteomics analysis.

PNGaseA-mediated N-glycan stripping from peptides by infant-derived Bifidobacterium bifidum

This paper characterizes a PNGaseA enzyme from Bifidobacterium bifidum that preferentially strips N-glycans from peptides over proteins, revealing its structural basis for substrate specificity.

Contrasting macroevolutionary patterns in the human N-glycosylation pathway

This study examines the evolutionary origins of glycosylation machinery genes and their targets in humans, revealing ancient and recent evolutionary patterns in the N-glycosylation pathway.

Tracking of in vivo O-GlcNAcylation in an Alzheimer's Disease and Aging C. elegans Model

The paper tracks O-GlcNAcylation changes in C. elegans models of Alzheimer's Disease and aging, showing age-dependent increases in wild-type worms and declines in the AD model, with altered trafficking dynamics in transgenic nematodes.

A triple fluorescence approach to measure O-GlcNAc dyshomeostasis in stem cells

This paper introduces a triple fluorescence stem cell sorting approach to measure O-GlcNAc dyshomeostasis and demonstrates its utility for high-throughput screening.

Comparative Evaluation of Glycoproteomics Software for Rare Glycopeptide Identification

This study evaluates five glycoproteomics software tools for identifying rare sialic acid-containing glycopeptides, finding that each tool has unique strengths and limitations in detection accuracy and reliability.

Retention of DLK1 in the endoplasmic reticulum identifies roles for EGF domain-specific O-glycans in the secretory pathway

This study investigates the role of EGF domain-specific O-glycans in the secretory pathway using DLK1 as a model, revealing distinct roles for O-fucose, O-glucose, and O-hexose modifications in ER transport.

ALG13 loss-of-function alters glycosylation, impairs neuronal maturation, and drives network hypoactivity in a cortical organoid model of CDG

This study uses cortical organoids to show that ALG13 loss-of-function causes glycosylation defects, neuronal maturation issues, and network hypoactivity in CDG.

Evolutionarily conserved temperature dependency leads to loss of protein O-GlcNAc in mammalian hypothermia

This study identifies temperature as an evolutionarily conserved regulator of O-GlcNAcylation, showing that hypothermia reduces O-GlcNAc levels across species.

O-Glycosylated RNA Identification and Site-specific Prediction by Solid-phase Chemoenzymatic TnORNA method and PONglyRNA tool

This paper identifies and predicts O-glycosylated RNA using a chemoenzymatic method and a computational tool, revealing their role in pancreatic cancer progression.

Transformer-based Deep Learning for Glycan Structure Inference from Tandem Mass Spectrometry

This paper introduces GlycoBERT and GlycoBART, transformer-based models for glycan structure prediction from MS/MS data, achieving high accuracy and enabling de novo structure inference.

GlycoRNA-L and glycoRNA-S mediate human monocyte adhesion via binding to Siglec-5

This study identifies glycoRNA-L and glycoRNA-S as mediators of human monocyte adhesion through binding to Siglec-5, with distinct glycan compositions and tissue expression patterns.

Conformational Dynamics and Membrane Insertion Mechanism of B4GALNT1 in Ganglioside Synthesis

This study reveals the conformational dynamics and membrane insertion mechanism of B4GALNT1 in ganglioside synthesis using crystal structures and molecular simulations.

Ultradeep N-glycoproteome Atlas of Mouse Reveals Spatiotemporal Signatures of Brain Aging and Neurodegenerative Diseases

The paper presents an ultradeep N-glycoproteome atlas of mouse tissues, revealing spatiotemporal patterns of glycosylation in brain aging and neurodegenerative diseases.

A genome-wide association study in 10,000 individuals links plasma N-glycome to liver disease and anti-inflammatory proteins

This study identifies 16 new genetic loci associated with plasma N-glycosylation in 10,000 individuals and links them to liver disease and inflammatory proteins.

A Unified Atlas of T cell Glycophysiology

This study characterizes T cell glycosylation patterns across mouse and human tissues, revealing species-specific differences in glycan profiles and dynamics.

The sialome of the retina, alteration in age-related macular degeneration (AMD) pathology and potential impacts on Complement Factor H

This study characterizes the sialome of the human retina and identifies alterations in age-related macular degeneration (AMD) that may impact complement factor H binding.

Chemoenzymatic Synthesis of Asymmetric Bisecting Bi-, Tri-, and Tetra-Antennary N-Glycans

This paper develops a chemoenzymatic method to synthesize asymmetrical bisecting N-glycans and investigates their binding properties with lectins and influenza viruses.

A High-Resolution N-Glycoproteome Atlas Reveals Tissue-Specific Glycan Remodeling but Non-random Structural Microheterogeneities

The paper presents a high-resolution N-glycoproteome atlas of 24 mouse tissues, revealing tissue-specific glycan remodeling and non-random structural microheterogeneities.

Serum Lewis fucosylation reports on survivorship of patients with septic shock upon admission to intensive care unit

This study identifies glyco-signatures in serum samples from septic shock patients that predict survival outcomes upon ICU admission using glycomics and machine learning.

GlyContact reveals structure-function relationships in glycans

GlyContact analyzes glycan 3D structures from MD, NMR, or X-ray data to understand structure-function relationships and improve AI models for lectin-glycan binding.

Metabolic Control of Glycosylation Forms for Establishing Glycan-Dependent Protein Interaction Networks

The paper introduces GAP-MS to study glycan-dependent PPIs and shows it identifies PPI variations under different glycosylation conditions.

An Integrative Approach to Develop and Characterise Antibodies Against the Cancer Associated Antigen Sialyl Lewis A (CA 19-9)

This paper develops and characterizes novel monoclonal antibodies against sLeA, showing improved affinity and specificity compared to existing antibodies.

Human parainfluenza virus infection remodels the host cell glycome

This study investigates how HPIV-3 infection alters the host cell glycome using mass spectrometry, revealing changes in N-glycans, O-glycans, and glycosphingolipids.

LM-GlycoRepo Version 1.0: A novel repository system for mouse tissue glycome mapping data

LM-GlycoRepo Version 1.0 is a repository for mouse tissue glycome mapping data that supports FAIR principles and includes an embargo system and visualization tool.

Molecular basis of Siglec-10 ligand recognition and antibody blockade

This study identifies key residues in Siglec-10 involved in ligand recognition and shows that antibody blockade can restore CAR-T cell cytotoxicity.

Integrated tumour-immune cell response modelling of Luminal A breast cancer details malignant signalling and ST3Gal1 inhibitor induced reversal

The paper develops a systems model of MUC1 glycosylation and immune interactions in Luminal A breast cancer, showing that sialyltransferase inhibition reverses cancerous signaling.

Evolutionary unique N-glycan-dependent protein quality control system plays pivotal roles in cellular fitness and extracellular vesicle transport in Cryptococcus neoformans

This study investigates the role of an N-glycan-dependent ERQC system in C. neoformans, showing its impact on virulence, cell surface organization, and extracellular vesicle transport.

Mgat4b mediated selective N-glycosylation regulates melanocyte development and melanoma progression

This study identifies Mgat4b as a regulator of melanocyte development and melanoma progression through N-glycosylation, showing its role in cell migration, adhesion, and tumor initiation.

Glycan analysis probes inspired by human lectins for investigating host-microbe crosstalk

The paper introduces glycan analysis probes (GAPs) inspired by human lectins to study host-microbe interactions and glycan-mediated processes.