Glycosylation and Glycoproteins Research

Extracting informative glycan-specific ions from glycopeptide MS/MS spectra with GlyCounter

GlyCounter is a tool to extract glycan-specific ions from glycopeptide MS/MS spectra, enabling analysis of glycan features without database searches.

Reaction kinetics of procainamide dye derivatization of N-linked glycans to enable robust process analytical workflows for glycoprotein-based biologics manufacturing

This study determines the reaction kinetics of procainamide labeling of N-glycans to improve analytical workflows for glycoprotein manufacturing.

Cytosolic mannosyltransferases involved in the ER N-glycosylation pathway exhibit bacterial ancestry

The paper investigates the evolutionary origins of ALG1 and ALG2 mannosyltransferases, finding that ALG2 likely originated from bacterial ancestors and ALG1 homologs are exclusive to bacterial lineages.

The Androgen Receptor and MYC synergise to modulate the synthesis of Siglec-7 ligands in prostate cancer

This study shows that the androgen receptor and MYC work together to control the production of Siglec-7 ligands in prostate cancer by regulating sialylation and glycopeptide profiles.

Identification and characterization of emNagⅡ, a novel β-1,2-N-acetylglucosaminidase from Elizabethkingia meningoseptica

This paper identifies and characterizes emNagⅡ, a β-1,2-N-acetylglucosaminidase from Elizabethkingia meningoseptica that removes β-1,2-linked N-acetylglucosamine from living cell surfaces.

Tissue-Specific Regulatory and Expression Patterns of CDG-causative genes account to Phenotypic Variability

The paper analyzes the expression and regulatory patterns of CDG-causative genes across tissues to explain phenotypic variability in congenital disorders of glycosylation.

Multi-domain O-GlcNAcase structures reveal allosteric regulatory mechanisms

This study presents multi-domain OGA structures from Trichoplax adhaerens and human, revealing allosteric regulatory mechanisms involving the pHAT domain.

Stromal cells modulate innate immune cell phenotype and function in colorectal cancer via the Sialic acid/Siglec axis

This study shows stromal cell sialylation via the Sialic acid/Siglec axis suppresses innate immune cell function in CRC, and targeting this pathway restores immune activity.

Multivariate analysis of glycogenes reveals coordinated regulation of immunoglobulin glycosylation in an immortalized human B cell system

The paper uses cytokine perturbations in B cells to analyze glycogene expression and IgG glycosylation, identifying cytokine-specific signatures and glycosylation profiles.

Spatial mapping of cell-surface protein glycosylation at molecular resolution

This paper introduces a super-resolution microscopy method to map cell-surface protein glycosylation at molecular resolution within the native cellular context.

Age-related remodeling of the glycocalyx drives T cell exhaustion

This study shows that aging reduces 2,6-linked sialic acids on T cells, leading to exhaustion and impaired immune function.

Active Site Heterogeneity Drives Differential Sialic Acid Metabolism in Enterobacteriaceae Pathogens

This study investigates how differences in active site structures of the NanA enzyme affect sialic acid metabolism in Enterobacteriaceae pathogens, revealing implications for pathogenicity and therapeutic targets.

Proteome-based investigation of O-GlcNAcylation in a C. elegans model of Ageing and Alzheimer's disease: Functional Support for Earlier Hypothesis-Generating Findings

This study identifies O-GlcNAc-modified proteins in C. elegans and a tauopathy model, showing developmental regulation and disruption in the model, with PDI-2 as a candidate regulator of proteostasis.

Hydroxyl and Trifluoromethyl Radical Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides

This paper introduces a novel method using radical carbohydrate footprinting to identify glycan-protein interactions and map binding interfaces by analyzing oxidation patterns of oligosaccharides.

A lectin-based magnetic CRISPR screening protocol permits rapid discovery of genes regulating cell-surface glycosylation

The paper introduces a lectin-based magnetic CRISPR screening protocol that enables faster and easier identification of genes regulating cell-surface glycosylation, applied to breast cancer hypersialylation.

Regulation of N-glycosylation efficiency by eukaryotic oligosaccharyltransferase

This paper identifies sequence determinants that regulate oligosaccharyltransferase (OST) catalytic efficiency and glycosylation specificity in proteins.

GlycoEnzDB: A database of enzymes involved in human glycosylation

GlycoEnzDB curates and presents human glycoenzyme data, linking enzyme functions, pathways, and expression data with external resources and AI-generated descriptions.

Absolute Quantification of Aging-Associated Glycans in IgG for Biological Age Prediction: Insights from Glycomics and Transcriptomics

This study quantifies absolute IgG glycan changes in aging and anti-aging models, identifies GP3 and GP8 as biomarkers, and develops a predictive model for biological age.

Identification and characterization of a novel exo-(N-)glycanase activity of NGLY1 on ENGase-digested N-GlcNAc proteins in vitro

This study identifies and characterizes a novel exo-(N-)glycanase activity of NGLY1 on N-GlcNAc proteins in vitro, expanding its functional repertoire and linking it to clinical features of NGLY1-CDDG.

Metabolic Crossroads: AMDHD2 Couples the Hexosamine Biosynthetic Pathway to Acetyl-CoA Homeostasis in Pluripotent Stem Cells

This study identifies AMDHD2 as a key regulator linking the hexosamine biosynthetic pathway to acetyl-CoA homeostasis in mouse embryonic stem cells, revealing a metabolic mechanism critical for differentiation.

Ex vivo and in vivo CRISPR/Cas9 screenings identify the roles of protein N-glycosylation in regulating T-cell activation and functions

The paper identifies B4GALT1 as a regulator of CD8+ T-cell function through N-glycosylation, showing its potential as a target for immunotherapy.

O-mannosylation and protein maturation check-points represent therapeutic opportunities in BRAF fusion protein oncogenesis

The paper identifies O-mannosylation and protein maturation as therapeutic targets in BRAF fusion oncogenesis by showing their necessity for fusion-induced transformation.

Evidence for Functional Regulation of the KLHL3/WNK Pathway by O-GlcNAcylation

This study shows that KLHL3 and WNK kinases are regulated by O-GlcNAcylation, affecting osmolarity control and ferroptosis, with implications for blood pressure and neuronal health.

The glycosyltransferase ALG3 is an AKT substrate that regulates protein N-glycosylation

This study shows that ALG3, a glycosyltransferase, is phosphorylated by AKT and regulates protein N-glycosylation, affecting cell proliferation and stress responses in cancer.

In-depth analysis of the tear fluid glycoproteome reveals diverse lacritin glycosylation and spliceoforms

This study uses mass spectrometry to analyze tear fluid glycoproteins, revealing novel glycosylation patterns and spliceoforms of lacritin and other clinically relevant proteins.

StCEL-based platform enables detection and sequencing of native sialoglycoRNA

The paper introduces StCEL, a method for detecting and sequencing native sialoglycoRNA, showing its upregulation in cancers and correlation with poor prognosis.

Dynamic Shielding and Allosteric Modulation of Erythropoietin by Glycosylation

This study uses molecular dynamics simulations to show that glycosylation modulates protein surfaces through both local shielding and long-range allosteric effects.

PNGaseL from Flavobacterium akiainvivens targets a diverse range of N-glycan structures

This study identifies PNGaseL from Flavobacterium akiainvivens as an enzyme with broad N-glycan specificity, outperforming PNGaseF and PNGaseA in cleaving diverse glycan structures.

Sialic Acid Identity Modulates Host Tropism of Sialoglycan-binding Viridans Group Streptococci

This study shows that sialic acid identity influences, but does not solely determine, host tropism of sialoglycan-binding streptococci by testing binding preferences of SLBRs to Neu5Ac and Neu5Gc.

Angstrom-resolution imaging of cell-surface glycans

This study visualizes individual sugars within glycans on the cell surface using angstrom-resolution fluorescence microscopy combined with metabolic labeling.