Cellular transport and secretion

Lipid transfer by ORP3 is required for the regulation of PI4P and PI(4,5)P2 at the plasma membrane in mitosis

This study shows that ORP3 lipid transfer is essential for regulating PI4P and PI(4,5)P2 levels at the plasma membrane during mitosis, affecting cell division and preventing genetic instability.

STX1A localizes to the lysosome and controls its exocytosis

This study identifies STX1A as a lysosomal SNARE that regulates exocytosis by localizing to lysosomes and plasma membrane, affecting lysosome number, dispersion, and proteolytic activity.

TMEDs Mediate Versatile Cargo Transport in Vesicle-dependent Unconventional Secretion

This study identifies TMED proteins as key regulators of vesicle-dependent unconventional secretion, showing they mediate cargo selectivity through translocation and oligomerization.

Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis

This study identifies physical interactions between septins and the exocyst complex in fission yeast, showing septins guide exocyst localization for proper cytokinesis.

The ARF regulatory GTPase in Giardia intestinalis is associated with vesicle formation and membrane fusion machinery at non-canonical endosomal compartments

The paper identifies Giardia intestinalis ARF GTPases and their regulators as key components of non-canonical endosomal compartments involved in vesicle formation and membrane fusion.

Endophilin-Lamellipodin-VASP, key components in fast endophilin-mediated endocytosis, control actin polymerization within liquid-like condensates.

This study identifies Endophilin, Lamellipodin, and VASP as key components in fast endophilin-mediated endocytosis that regulate actin polymerization through liquid-like condensates.

Glutamic acid-lysine (EK) rich motif of RabD2 self-associates and regulates pathogenesis through multivesicular bodies pathway in E. histolytica

This paper identifies RabD2 in E. histolytica, showing it self-associates via an EK-rich motif and regulates MVB biogenesis, leading to decreased parasite adherence through ubiquitination of GalNAc lectin.

Of condensates and coats - reciprocal regulation of clathrin assembly and the growth of protein networks

This study shows that protein condensates and clathrin assembly regulate each other during clathrin-mediated endocytosis, enhancing vesicle formation while controlling condensate growth.

Loss of ErbB3 redirects Integrin β1 from early endosomal recycling to secretion in extracellular vesicles

The paper shows that ErbB3 regulates Integrin β1 trafficking, redirecting it from recycling to lysosomal degradation and EV secretion when ErbB3 is lost, affecting cell migration.

Mechanical Cues Regulate Cargo Sorting and Export at the Golgi

This study identifies a mechanical regulation pathway involving Src-FAK-AMPK-GBF1 that controls cargo sorting and secretion from the Golgi in response to substrate stiffness.

CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

The paper identifies CROP2 as a Retriever-WIPI2 complex involved in exporting β1-Integrin from endosomes to the plasma membrane, distinct from the CROP complex.

Quantum Tunneling-Gated Vesicle Fusion: Proton-Coupled Electron Transfer and Mechanical Barrier Softening Shape Neurotransmitter Release Latency

The paper introduces a quantum-mechanochemical model to explain neurotransmitter release latency by combining proton-coupled electron transfer and mechanical barrier softening.

The mechanistic basis of cargo selection during Golgi maturation

This study determines the structure of the COPI vesicle coat with GOLPH3 and shows how it preferentially binds Golgi residents at late cisternae through coincidence detection of COPI and lipid.

Close-Up of vesicular ER Exit Sites by Volume Electron Imaging using FIB-SEM

The paper uses FIB-SEM and ASEM to identify and characterize vesicular ER exit sites in mammalian cells, revealing their abundance and organization.

Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7

This study identifies the RAB32-LRMDA-Commander complex and shows its role in melanosome biogenesis and pigmentation, revealing the mechanism of oculocutaneous albinism type 7.

Mechanism of phosphoinositide regulation of lysosomal pH via inhibition of CLC-7

The paper investigates how PI(3,5)P2 regulates lysosomal pH by inhibiting ClC-7, showing structural changes in ClC-7 upon PI(3,5)P2 binding and linking this to disease mutations.

Mapping EGFR1 sorting domains in endosomes with a calibrated 3D expansion microscopy toolkit

This paper develops a 3D expansion microscopy toolkit to visualize and quantify nanoscale interactions in endosomes, revealing EGF and EGFR1 enrichment and Rab5a accumulation during endosomal maturation.

TRIM62 promotes lipid raft-mediated entry of influenza A virus by regulating WASH localization

This study identifies TRIM62's role in facilitating influenza A virus entry via lipid rafts by modulating WASH and VPS35 interactions.

Harnessing fusion of genome-edited human stem cells to rapidly screen for novel protein functions in vivo

This paper uses cell fusion to rapidly generate genome-edited human cells expressing fluorescent fusion proteins, revealing novel protein-organelle interactions.

Structural remodeling of target-SNARE protein complexes by NSF enables synaptic transmission

The paper reveals how NSF and SNAP proteins remodel SNARE complexes to enable synaptic transmission by disassembling syntaxin clusters through ATP hydrolysis.

Inseparable/IER3IP1 are essential for cytokinesis in Drosophila neuroblast and human cells

This study identifies Insep and IER3IP1 as essential for cytokinesis, showing their conserved role in Rab11 vesicle dynamics across Drosophila and human cells.

RAB14-dependent tubulovesicular recycling directs MET to invadopodia promoting TNBC cell invasion

This study identifies RAB14-dependent tubulovesicular recycling as a mechanism for MET delivery to invadopodia, enhancing TNBC cell invasion through matrix degradation.

Spontaneous mutant in threespine stickleback connects endosome trafficking disorders and inflammatory bowel diseases via changes in the gut microbiome

The paper uses a mutant fish model to show endosome trafficking disorders link to gut microbiome changes and inflammatory bowel disease.

Transcytosis-mediated anterograde transport of TrkA receptors controls formation of presynaptic sites in sympathetic neurons

This study shows transcytosis of TrkA receptors in sympathetic neurons is essential for presynaptic site formation and synaptic transmission.

Phagosome Maturation in Macrophages is Enhanced by p38α MAPK Signaling

The paper shows that specific ligands on phagocytosed cargo activate p38 MAPK signaling, enhancing phagosome maturation and lysosomal delivery in macrophages.

Nanoscopy of Organelle Handoff Portals Reveals Direct Coupling between ER Remodeling and Microtubule-Based Transport

The paper uses nanoscopy to show ER remodeling and microtubule-based transport are directly coupled through nanoscopic hubs and membrane tethers.

Characterisation of LAMP1- and LAMP2A-positive organelles in neurons

The paper characterises LAMP1- and LAMP2A-positive organelles in neurons, finding they have distinct transport dynamics and protein compositions.

De novo Golgi biogenesis requires coordinated transactivation of a Golgi regulon

The paper identifies a Golgi regulon and shows CREB3L1 drives its transcriptional activation during de novo Golgi biogenesis.

Non-synaptic exocytosis along the axon shaft and its regulation by the submembrane periodic skeleton

The paper identifies non-synaptic exocytosis along axon shafts and shows how the submembrane actin-spectrin skeleton regulates this process.

The RING-finger domain of Arabidopsis RMR functions as an E3 ligase essential for post-Golgi trafficking

This study identifies the RING-H2 domain of Arabidopsis RMR as an E3 ligase required for post-Golgi trafficking of RMR receptors.