Cellular transport and secretion

Loss of CHMP2A implicates an ordered assembly of ESCRT-III proteins during cytokinetic abscission

This study shows that CHMP2A is essential for the ordered assembly of ESCRT-III proteins during cytokinetic abscission, revealing a hierarchical organization of these subunits.

The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development

The paper identifies a mechanism where axonal ER couples translation and secretion of transmembrane proteins to support axonal development.

Nitric oxide regulates phagocytosis through S-nitrosylation of Rab5

This study shows that nitric oxide promotes phagocytosis by S-nitrosylating Rab5, enhancing its activity and bacterial clearance in macrophages.

Late endosome transport by RILP-RAB7A promotes dendrite arborization

This study shows that RILP-RAB7A-mediated late endosome transport is essential for dendrite arborization, independent of its role in degradation.

Adaptor protein complex 2 (AP2) participates in biogenesis and homeostasis of myelin sheaths in the central nervous system

This study shows that AP2 complex is essential for myelin sheath biogenesis and homeostasis, with loss of AP2 causing hypomyelination and hypermyelination in different developmental stages.

Selectivity profiles and substrate recognition of Rab phosphorylating kinases

The paper identifies novel kinase:Rab pairs and characterizes substrate recognition determinants for Rab phosphorylation by kinases like LRRK2.

Structure of Pex8 in complex with peroxisomal receptor Pex5 reveals its essential role in peroxisomal cargo translocation

This study reveals the structural and functional role of Pex8 in peroxisomal cargo translocation by interacting with Pex5, facilitating receptor recycling.

Endocytic Patch Dynamics are Differentially Regulated at Distinct Cell Sites in Fission Yeast

The paper shows that endocytic patch dynamics in fission yeast are differentially regulated at cell ends, division site, and cell sides by Cdc42 and Pak1 kinase.

Pathogenic mutations in Clathrin Heavy Chain associated with intellectual disability impair synaptic architecture and learning in Drosophila

Ligand-Mediated Endocytosis Is Regulated in a Sexually Dimorphic Way in Osteocytes in vivo

The paper studies endocytosis in osteocytes in vivo, finding sex-specific differences in dynamin-dependent endocytosis and nanoparticle uptake patterns.

Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis

This study shows Epsin1 stabilizes initiator protein condensation during clathrin-mediated endocytosis only when ubiquitin is present, ensuring cargo-specific vesicle formation.

Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network.

The paper investigates Eps15's EH domains interactions with Dab2 and its own IDR, revealing promiscuous binding and a self-inhibitory mechanism that facilitates endocytosis.

Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol

This study shows that Sac1 regulates Golgi V-ATPase assembly by controlling TGN membrane composition through lipid exchange, leading to Golgi fragmentation and impaired glycosylation.

PKD3 localizes to late endosomes to maintain Rab7-dependent endolysosomalhomeostasis

This study identifies PKD3's localization to Rab7-positive endosomes and shows its role in maintaining endolysosomal homeostasis, which supports cancer stem cell maintenance in TNBC.

Vacuolar pH regulates clathrin-mediated endocytosis through TORC1 signaling during yeast replicative aging

Combined thermodynamic and time-resolved structural analysis of interactions between AP2 and biomimetic plasma membranes provides insights into clathrin-mediated endocytosis

This study uses biophysical methods to analyze AP2 interactions with biomimetic membranes, revealing insights into clathrin-mediated endocytosis mechanisms.

Golgi retention of KIT in gastrointestinal stromal tumour cells is phospholipase D activity-dependent

This study shows that KIT mutant retention in the Golgi/TGN in GISTs depends on the PLCγ2-PKD2-PLD2 pathway, leading to signal inactivation upon PLD inhibition.

Opposing actions of JIP4 and RILPL1 provide antagonistic motor force to dynamically regulate membrane reformation during lysosomal tubulation/sorting driven by LRRK2

The paper identifies JIP4 and RILPL1 as opposing motor adaptors regulating lysosomal tubulation through differential microtubule transport.

Two successive oligomeric Munc13 assemblies scaffold vesicle docking and SNARE assembly to support neurotransmitter release

This study shows that two Munc13 oligomers are essential for vesicle docking and SNARE assembly, supporting neurotransmitter release.

The bacterial ESCRT-III PspA rods thin lipid tubules and increase membrane curvature through helix α0 interactions

This study shows how PspA rods thin lipid tubules by inducing membrane curvature through N-terminal helix 0 interactions, overcoming bending energy via molecular mechanisms.

GTP hydrolysis triggers membrane remodeling by AMPH-1

This study shows that GTP hydrolysis by AMPH-1 drives membrane remodeling through oligomerization and tubulation, essential for transport carrier formation.

Structural basis of pseudoGTPase-mediated protein-protein interactions

This study identifies the AAGAB pseudoGTPase domain as a novel protein-protein interaction module that interacts with AP1 and AP2 σ subunits, revealing a unique structural interface distinct from conventional GTPase interactions.

Depletion of endomembrane reservoirs drives phagocytic appetite exhaustion in macrophages

This study identifies endomembrane depletion as a key factor in phagocytic capacity exhaustion in macrophages.

Surface delivery quantification reveals distinct trafficking efficiencies among clustered protocadherin isoforms

The paper quantifies surface delivery differences among clustered protocadherin isoforms and variants, revealing that cis dimerization is not tightly coupled to surface delivery.

DNAJC13 localization to endosomes is opposed by its J domain and its disordered C-terminal tail

This study identifies how DNAJC13's endosomal localization is regulated by its J domain and C-terminal tail through interactions with PI(3)P and oligomerization.

Cathartocytosis: Jettisoning of Unwanted Material during Cellular Reprogramming

The paper describes a novel lysosome-independent process called cathartocytosis, where cells eject unwanted material during reprogramming, distinct from autophagy.

PEX39 facilitates the peroxisomal import of PTS2 proteins

This study identifies PEX39 as a cytosolic peroxin that facilitates PTS2-protein import by interacting with PEX7 and stabilizing its binding to cargo.

Mapping the endosomal proximity proteome reveals Retromer as a hub for RAB GTPase regulation.

The paper identifies Retromer as a hub for RAB GTPase regulation through proximity proteomics and biochemical analysis.

Hybrid endosomal coats containing different classes of sorting nexins

The paper shows that Snx3 and SNX-BARs form hybrid endosomal coats in vivo, which have greater membrane scaffolding activity than homogeneous coats and allow for adaptable cargo sorting.

Cargo-selective regulation of clathrin-mediated endocytosis by AMP-activated protein kinase

This study shows AMPK regulates clathrin-mediated endocytosis by controlling Dab2 and Arf6 to selectively internalize β1-integrin.