Trypanosoma species research and implications

Identification of antifungal agents AR-12 and Fosmanogepix as anti-Trypanosoma cruzi drugs through an enhanced fluorogenic β-galactosidase phenotypic screening assay

This paper develops a fluorogenic β-galactosidase assay to screen antifungal compounds against Trypanosoma cruzi, identifying AR-12 and Fosmanogepix as potential drugs.

An auxin-inducible degron system for trypanosomes

This paper describes the adaptation of an auxin-inducible degron system for trypanosomes by expressing a rice E3 ubiquitin ligase to enable specific protein degradation.

Insights from aquaporin structures into drug-resistant sleeping sickness

This study determines the structure of TbAQP2 bound to drugs and glycerol, revealing how mutations in the channel lead to drug resistance in Trypanosoma brucei.

Origins and consequences of kinetoplast loss in trypanosomes

This paper identifies mutations in the gamma subunit of the mitochondrial ATP synthase in Trypanosoma brucei that lead to kinetoplast loss and drug resistance.

Randomized field trial of a therapeutic vaccine against Trypanosoma cruzi natural infection in dogs and correlates for efficacy

This paper reports a randomized field trial of a therapeutic vaccine against Trypanosoma cruzi in dogs, showing reduced parasite burden and prevention of cardiac disease.

Mutations in the HKD motif of Trypanosoma brucei cardiolipin synthase inhibit cardiolipin synthesis and parasite growth

This study shows that mutations in the HKD motif of TbCls in Trypanosoma brucei disrupt cardiolipin synthesis and parasite growth.

vsgseq2: an updated pipeline for analysis of the diversity and abundance of population-wide Trypanosoma brucei VSG expression

vsgseq2 improves the analysis of VSG expression diversity and abundance in Trypanosoma brucei populations using updated bioinformatics tools.

Neotropical bats as bioindicators for emerging zoonoses in Central America: A case study identifying Trypanosoma cruzi in bats from Belize using metagenomic next-generation sequencing

The paper uses metagenomic sequencing and qPCR to detect Trypanosoma cruzi in bats from Belize, demonstrating their role as bioindicators for emerging zoonoses.

The first RNA viruses detected in a trypanosome: novel narnaviruses and a leishmaniavirus in the gecko parasite Trypanosoma platydactyli

The paper identifies and characterizes the first RNA viruses in trypanosomes, including a leishmaniavirus and two narnaviruses in Trypanosoma platydactyli, suggesting ecological factors influence viral acquisition.

Acoziborole resistance associated mutations in trypanosome CPSF3

The paper identifies mutations in trypanosome CPSF3 that confer resistance to acoziborole, a drug targeting RNA processing, and explains the molecular mechanism of resistance through computational modeling.

Chromosome compartment assembly is essential for subtelomeric gene silencing in trypanosomes

This study shows that chromosome compartment assembly is essential for subtelomeric gene silencing in Trypanosoma brucei, with RAP1 and phosphoinositides regulating this process.

Extracellular glutamine is essential in cell division and flagellar function in bloodstream forms of Trypanosoma brucei

This study shows that extracellular glutamine is critical for cell division and flagellar function in Trypanosoma brucei bloodstream forms, with its absence leading to polyploid, multiflagellated cells and proteome glutamylation changes.

Development and pilot application of a point-of-need molecular xenomonitoring protocol for tsetse (Glossina sp.) in a low-resource setting

The paper develops and tests a low-cost, point-of-need molecular xenomonitoring protocol for tsetse flies in a low-resource setting, achieving high DNA extraction success and pathogen detection rates.

The evolutionary origin of host association and polycistronic transcription in trypanosomatids

This study investigates the evolutionary origins of host association and polycistronic transcription in trypanosomatids using comparative genomics across 47 genomes.

Untangling the diversity of Trypanosomes infecting Colombian amphibians: morphometric and molecular insights

This study identifies Trypanosoma species in Colombian amphibians using morphometric and molecular analyses, revealing new species and expanding known ranges.

Induced pluripotent stem cell-derived human macrophages as an infection model for Trypanosoma cruzi

This paper uses human iPSC-derived macrophages to model T. cruzi infection and test trypanocidal compounds for Chagas disease drug discovery.

Cure of experimental Trypanosoma vivax infection with a single dose of an unmodified antibody-based drug targeting the invariant flagellum cell surface protein IFX

This paper shows that a single dose of an unmodified anti-IFX monoclonal antibody can cure T. vivax infections in mice, with no detectable parasites in peripheral tissues.

Identification of small molecule inhibitors of Trypanosoma PEX15--PEX6 interaction

This study identifies small molecule inhibitors targeting the TbPEX6-TbPEX15 interaction in trypanosomatid parasites, showing trypanocidal activity in vitro.

First Genome-Wide Centromere Map of Trypanosoma cruzi Reveals Linear and 3D Compartment Boundaries and Spatial Clustering

The paper generates a high-quality genome assembly and centromere map for T. cruzi using Hi-C and ChIP-seq, revealing structural boundaries and 3D clustering of centromeres.

Targets of the human antibody response to the variant surface glycoprotein of Trypanosoma brucei

This study uses PhIP-seq to analyze anti-VSG antibody responses in HAT patients, revealing differences between acute and chronic forms and identifying diagnostic targets.

A non-coding role for trypanosome VSG transcripts in allelic exclusion

The paper shows that VSG transcripts play a non-coding role in allelic exclusion in trypanosomes by participating in allelic competition.

Placental transcriptome profiling in congenital Chagas disease: gene networks associated with transmission

The paper identifies gene expression differences in placental tissues from congenitally infected, exposed but uninfected, and unexposed mothers to understand molecular mechanisms of Chagas disease transmission.

Modelling framework to demonstrate elimination of a vector population: tsetse elimination in Chad

The paper develops a modelling framework to assess tsetse fly elimination in Chad, showing that continued sampling without detections could confirm elimination with 99% confidence.

Transcriptomic and proteomic analysis of quiescent epimastigotes as a resource for investigating Trypanosoma cruzi persistence.

The paper analyzes the transcriptome and proteome of quiescent T. cruzi epimastigotes to understand persistence mechanisms and improve drug development.

Assembly of the mitochondrial outer membrane module of the trypanosomal tripartite attachment complex

The paper identifies four soluble subcomplexes of the outer mitochondrial membrane TAC module in Trypanosoma brucei, suggesting a branched assembly pathway involving a ring-like intermediate.

The role of the Trypanosoma cruzi enzyme L-threonine 3-dehydrogenase in combating stressful environments

This study shows that overexpressing L-threonine 3-dehydrogenase (TDH) in Trypanosoma cruzi increases survival under oxidative stress and drug exposure, and improves infection capacity.

A genome-wide overexpression screen identifies TbFOP, whose overexpression disrupts transcription termination in Trypanosoma brucei

This study identifies TbFOP as a gene whose overexpression disrupts transcription termination and causes cell-cycle defects in Trypanosoma brucei.

Essential roles of HGT-derived sterol biosynthesis in trypanosomatid growth and parasitism

This study identifies HGT-derived genes critical for sterol biosynthesis in trypanosomatids, showing how gene loss and retention during parasitism shape metabolic adaptations.

Engineered heat-stable variants of Trypanosoma cruzi flagellar protein Tc24 enable serological detection of Chagas disease

The paper engineers heat-stable variants of Tc24, a Trypanosoma cruzi antigen, enabling serological detection of Chagas disease without refrigeration.

Research advance: Unexpected plasticity in the life cycle of Trypanosoma brucei

This study shows slender Trypanosoma brucei can infect both teneral and non-teneral tsetse flies without supplements, and remains transcriptionally distinct from stumpy forms during differentiation.