Viral gastroenteritis research and epidemiology

Integrative Structure of Norovirus NS3 Suggests a Role in RNA Transport

The paper determines the structure of norovirus NS3 and proposes it functions as an RNA translocase.

Mechanism of membrane perforation in rotavirus cell entry

This study uses cryogenic electron tomography to visualize the mechanism of membrane perforation during rhesus rotavirus entry, linking structural data with live-cell imaging.

Conformational plasticity of the human norovirus GII.3 capsid reveals alternative P domain interaction networks

This study identifies two distinct capsid conformations in human norovirus GII.3 virus-like particles, revealing conformational switching and alternative P domain interaction networks.

Viral protease-mediated polyprotein processing in human astroviruses

This study identifies cleavage sites and processing dynamics of astrovirus polyproteins, revealing conserved and divergent features between classical and non-classical strains.

Efficient plasmid-based rescue of T7 RNA polymerase-driven calicivirus reverse genetics systems in mammalian cells using vaccinia virus RNA capping enzymes

This paper introduces a plasmid-based system using vaccinia virus capping enzymes to enhance calicivirus reverse genetics in mammalian cells, significantly increasing viral titres and polyprotein abundance.

A single amino acid mutation in norovirus NS4 promotes viral spread

This study identifies a single amino acid mutation in norovirus NS4 that rescues replication defects of a specialist virus, enabling it to spread more efficiently in vitro.

Characterization of high affinity IgM and IgG monoclonal antibodies against norovirus variants GII.4 and GII.17

This study generates and characterizes monoclonal IgM and IgG antibodies against norovirus variants GII.4 and GII.17, revealing differences in binding mechanisms and affinity.

Proof of concept: Targeted protein degradation of the stress granules component G3BP1 as an antiviral strategy against norovirus infection

This study demonstrates that targeting G3BP1 for proteolysis using PROTACs reduces norovirus replication and prevents cell death.

Mutational Analysis of Human Norovirus VP2 Elucidates Critical Molecular Interactions for Virus Assembly

This paper identifies critical molecular interactions of VP2 in HuNoV assembly through mutational analysis and computational modeling.

Calicivirus assembly and stability are mediated by the N-terminal domain of the capsid protein with the involvement of the viral genome

This paper investigates the role of the N-terminal domain of the capsid protein in calicivirus assembly and stability, showing its interaction with the viral genome and its impact on capsid structure and mechanical properties.

Structural Hijacking of FcRn by Human Astrovirus Spikes Reveals Conserved Epitopes for Broad-Spectrum Antivirals

This study determines the structure of HAstV spikes bound to FcRn, identifies conserved epitopes, and suggests repurposing FcRn inhibitors for antiviral therapy.

Trim47 inhibits murine norovirus replication in a strain-dependent manner

This study identifies Trim47 as a host restriction factor that inhibits murine norovirus strain CR6 but not strain CW3, revealing strain-specific antiviral mechanisms.

Comparative analysis of NSP5/VP2-induced viroplasm-like structures in rotavirus species A to J

This study compares NSP5/VP2-induced viroplasm-like structures across rotavirus species A-J, identifying species-specific differences and conserved mechanisms in VLS formation.

Functional Diversity in GII.4 Norovirus Entry: HBGA Binding and Capsid Clustering Dynamics

The paper compares GII.4 norovirus variants to determine if clustering and entry mechanisms are conserved, finding clustering enhances entry and is mediated by specific residues and lipid rafts.

Endoplasmic reticulum-anchored nonstructural proteins drive human astrovirus replication organelle formation

This study identifies viral proteins involved in HAstV replication organelle formation by analyzing ER restructuring and DMV networks using microscopy techniques.

The Leader of the Capsid protein from Feline calicivirus must be palmitoylated and form oligomers through disulfide bonds for efficient viral replication

This study identifies that the LC protein from Feline calicivirus requires palmitoylation and disulfide bond-mediated oligomerization for efficient viral replication.

Structural determinants of rotavirus proteolytic activation

This study identifies structural constraints in rotavirus spike protein loops that regulate proteolytic activation necessary for infectivity.

Egress-enhancing mutation reveals inefficiency of non-enveloped virus cell exit

The paper identifies an egress-enhancing mutation in murine norovirus that increases replication speed and viral titers in mice.

Development of a replication competent murine norovirus reporter system

This paper develops a luciferase-based reporter system for murine norovirus to study replication and screen antiviral compounds.

Murine norovirus allosteric escape mutants mimic gut activation.

This paper identifies allosteric escape mutants of murine norovirus that constitutively adopt the activated conformation, providing insights for vaccine design.

Membrane asymmetry facilitates murine norovirus entry and persistent enteric infection

This study shows that lipid asymmetry, mediated by TMEM30a, is essential for murine norovirus entry and persistent infection.

A Single Mutation in an Enteric Virus Alters Tropism and Sensitivity to Microbiota

This study shows a single amino acid change in a reovirus alters its infection outcome in mice with microbiota depletion, affecting tropism and sensitivity to microbiota-driven antiviral responses.

Conformational flexibility is a critical factor in designing broad-spectrum human norovirus protease inhibitors

This study identifies conformational flexibility in GII proteases as a key factor affecting inhibitor potency, guiding the design of broad-spectrum norovirus protease inhibitors.

Discovery of three novel neutralizing antibody epitopes on the human astrovirus capsid spike and mechanistic insights into virus neutralization

This study identifies three novel neutralizing antibody epitopes on the human astrovirus capsid spike using structural biology techniques and shows their role in blocking virus neutralization.

Viroporin activity from rotavirus nonstructural protein 4 induces intercellular calcium waves that contribute to pathogenesis

This study identifies NSP4 as a viroporin that induces intercellular calcium waves, contributing to rotavirus virulence through innate immune activation and disease severity.

2'-Fucosyllactose Inhibits Human Norovirus Replication in Human Intestinal Enteroids

This paper shows that 2'-fucosyllactose inhibits replication of a dominant human norovirus strain in intestinal enteroids from adult and pediatric donors.

Importance of a heat snap in RT-PCR quantification of rotavirus double-stranded RNA in wastewater

This study shows that using a heat snap in RT-PCR increases rotavirus RNA quantification in environmental samples by 125-fold across different sample types and laboratories.

The neonatal Fc receptor and DPP4 are human astrovirus receptors

This study identifies FcRn and DPP4 as human astrovirus receptors and shows that targeting these factors with existing inhibitors prevents infection in human enteroid cultures.

Insights into Human Norovirus Cultivation in Human Intestinal Enteroids

This paper reports the cultivation of human noroviruses in human intestinal enteroids, revealing segment- and strain-specific differences in viral replication and highlighting the utility of genetically modified enteroids for broader strain cultivation.

Metabolic immaturity of newborns and breast milk bile acids are the central determinants of heightened neonatal vulnerability to norovirus diarrhea

This study identifies metabolic immaturity in newborns and maternal bile acids in breast milk as key factors increasing vulnerability to norovirus diarrhea.