Photoreceptor and optogenetics research

Optogenetic control of medaka behavior with channelrhodopsin

This study introduces a method to control medaka behavior using optogenetics with channelrhodopsin, showing light-induced movements in embryos, larvae, and adults.

Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors

This paper optimizes optogenetically functionalized GPCRs to achieve specific in vivo neuromodulatory signaling in Drosophila, showing rescue of endogenous receptor function.

Starburst amacrine cells amplify optogenetic visual restoration through gap junctions in the murine retina

The paper demonstrates that optogenetic actuators in starburst amacrine cells enhance visual restoration in murine retinas through gap junctions.

Optogenetic determination of dynamic and cell-type-specific chloride equilibrium potentials

The paper uses an optogenetic approach to measure dynamic and cell-type-specific chloride equilibrium potentials in rodent neurons.

Optogenetic inhibition of Gα signalling alters and regulates circuit functionality and early circuit formation

This paper develops and validates an optogenetic tool, PiGM-Iq, to inhibit Gq signaling and demonstrates its effects on behavior, axon guidance, and neuronal development in multiple organisms.

All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals

This paper introduces an optogenetic voltage-clamp technique for precise control of cellular voltage in live animals, demonstrating its application in C. elegans and rat neurons.

Ultrafast Light Targeting for High-Throughput Precise Control of Neuronal Networks

This paper introduces FLiT, a method for ultra-fast sequential light targeting to enable precise and scalable control of neuronal networks.

Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator

The paper identifies a mutation that enhances the sensitivity of a fluorescent voltage indicator, improving its ability to detect both spiking and subthreshold voltage signals in multiple organisms.

Surgical procedure for implantation of Opto-Array in nonhuman primates

This paper describes a surgical method for implanting an Opto-Array in nonhuman primates to enable high-throughput optogenetic stimulation and behavioral testing.

Bicistronic expression of a high-performance calcium indicator and opsin yields stable, robust cortical expression for holographic two-photon stimulation

The paper introduces a bicistronic AAV vector that co-expresses GCaMP8s and stChrimsonR in mice, enabling stable and robust two-photon holographic stimulation and imaging of cortical neurons.

Light inducible protein degradation in E. coli with the LOVdeg tag

This paper introduces LOVdeg, a light-inducible protein degradation tag for E. coli, demonstrating its modularity and utility in optogenetic control.

Green, orange, red, and far-red optogenetic tools derived from cyanobacteriochromes

The paper develops optogenetic tools that control protein-protein interactions using light in multiple colors by engineering binders for different states of cyanobacteriochromes.

Graded optogenetic activation of the auditory pathway for hearing restoration

This paper develops a framework for optogenetic control of the auditory pathway by optimizing light pulse parameters to enhance spike probability and information encoding in spiral ganglion neurons.

Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles

This paper introduces optoSynC, an optogenetic tool that rapidly and reversibly silences synaptic transmission by clustering synaptic vesicles in C. elegans.

Enhancing the performance of Magnets photosensors through directed evolution

This paper improves the light sensitivity of Magnets photosensors through directed evolution, enabling tunable optogenetic regulation in bacteria and mammalian cells.

Optogenetic Protein Cleavage in Zebrafish Embryos

This paper demonstrates the use of PhoCl, a photocleavable fluorescent protein, to achieve optical control over protein cleavage in zebrafish embryos, enabling light-triggered protein translocation and apoptosis.

Photocycle characterization of a blue-orange cyanobacteriochrome from Synechococcus sp. PCC 7002

The paper characterizes the photocycle of a blue-orange cyanobacteriochrome in Synechococcus sp. PCC 7002, showing it cycles between orange and blue light-absorbing states with specific chromophore isomers and cysteine residues involved.

Cryo-EM structures of the channelrhodopsin ChRmine in lipid nanodiscs

The paper presents cryo-EM structures of ChRmine, a microbial channelrhodopsin, in lipid nanodiscs, revealing its trimeric architecture and structural basis for its unique optogenetic properties.

Optogenetic control of cell signaling with red/far-red light-responsive optogenetic tools in Caenorhabditis elegans.

This study introduces a red/far-red light-responsive optogenetic system in C. elegans to manipulate cell signaling without blue light avoidance behavior.

3-D optogenetic control of arteriole diameter in vivo

This paper develops an optogenetic method to control arteriole diameter in 3D with high spatiotemporal resolution, enabling study of vascular dynamics and blood flow regulation.

Implementation of a novel optogenetic tool in mammalian cells based on a split T7 RNA polymerase

The paper implements and optimizes a novel optogenetic tool in mammalian cells using a split T7 RNA polymerase system for light-inducible gene expression control.

WiChR, a highly potassium selective channelrhodopsin for low-light two-photon neuronal inhibition

The paper identifies a highly potassium-selective channelrhodopsin (WiChR) that enables efficient neuronal inhibition through two-photon optogenetics under low-light conditions.

Improved Version of ChETA Promotes Aggression in the Medial Amygdala

The paper shows that an improved ChETA variant (ChETATC) promotes aggression in mouse medial amygdala GABAergic neurons, suggesting previous results with ChETA were due to weaker photocurrents and stimulation conditions.

A Neuropsin-based Optogenetic Tool for Precise Control of Gq signaling

This paper introduces a neuropsin-based optogenetic tool, cOpn5, that enables precise control of Gq signaling with high temporal and spatial resolution.

Additive and epistatic effects influence spectral tuning in molluscan retinochrome opsin

This study shows that mutations in retinochrome opsin's binding pocket cause spectral tuning through epistatic interactions rather than additive effects.

Optogenetic stimulation of medial amygdala GABA neurons with kinetically different channelrhodopsin variants yield opposite behavioral outcomes

The paper compares the behavioral effects of two optogenetic channelrhodopsin variants, finding that ChETA suppresses aggression while ChR2 increases it in mice.

A pragmatic reevaluation of the efficacy of nonhuman primate optogenetics for psychiatry

The paper reevaluates the efficacy of nonhuman primate optogenetics for psychiatry, finding lower success rates when applying more relevant criteria to human neuropsychiatric disease.

Restoration of high-sensitivity patterned vision in motion with an engineered light-gated G protein-coupled receptor

This paper demonstrates a chemical-optogenetic approach that restores high-sensitivity motion vision in an IRD mouse model using a multi-branched photoswitch.

Coupling a Live Cell Directed Evolution Assay with Coevolutionary Landscapes to Engineer an Improved Fluorescent Rhodopsin Chloride Sensor

The paper engineers an improved fluorescent chloride sensor using directed evolution and coevolutionary analysis in E. coli.

Temporally extended and reversible neuronal silencing with Aion

The paper introduces Aion, a bistable anion-conducting channelrhodopsin with a long open state and precise light-switchable control, enabling extended neuronal silencing in Drosophila.