Advanced Fluorescence Microscopy Techniques

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

The paper compares monomeric StayGold variants using protein nanocages in living cells, finding mStayGold is 3-fold brighter and more photostable than EGFP and other variants.

A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging

This paper introduces a low-cost FPGA-based method to correct pile-up distortions in TCSPC-FLIM, enabling high-speed, accurate in vivo imaging with subcellular resolution.

Image-based 3D active sample stabilization on the nanometer scale for optical microscopy

This paper introduces an image-based 3D active stabilization method for super-resolution microscopy, achieving ~1 nm stability without requiring fiducial markers or complex hardware.

GEQO biosensors for absolute analyte quantification in single cells

The paper introduces GEQO biosensors for absolute analyte quantification in single cells without requiring specialized equipment, showing they account for buffering and retain time trace information.

A hierarchical adaptive optics strategy for three-photon imaging during behavior

This paper introduces a hierarchical adaptive optics strategy to improve three-photon imaging in the mouse brain during behavior by correcting optical aberrations at multiple levels.

Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions

This paper corrects optical aberrations in GRIN lens-based microendoscopes to improve imaging performance in deep brain regions.

Accurate tracking of lytic granules via simultaneous particle tracking, phase retrieval and point spread function reconstruction

This paper develops a framework for simultaneous particle tracking, phase retrieval, and PSF reconstruction to improve 3D tracking of lytic granules in T cells.

A preprocessing toolbox for 2-photon subcellular calcium imaging

The paper introduces a preprocessing pipeline for 2-photon calcium imaging to improve subcellular signal detection and ROI grouping.

Real-time affinity measurements of proteins synthesized in cell-free lysate using fluorescence correlation spectroscopy

This paper develops a method to measure protein-protein interactions in real-time using cell-free synthesis and fluorescence correlation spectroscopy, achieving high sensitivity and throughput.

I2SIM: Boosting High-Fidelity Isotropic Super-Resolution with Image Interference and Spatial-Spectrum Optimization

I2SIM improves axial resolution in 3D-SIM by using interference and spectral optimization, achieving 130 nm resolution for subcellular structures.

Fast adaptive super-resolution lattice light-sheet microscopy for rapid, long-term, near-isotropic subcellular imaging

This paper introduces Meta-rLLS-VSIM, a super-resolution microscopy technique that achieves near-isotropic resolution with reduced training time and data requirements.

Overcoming Fluorescence Loss in mEOS-based AAA+ Unfoldase Reporters Through Covalent Linkage

The paper develops a method to use mEOS-based reporters for membrane-associated AAA+ unfoldases by covalently linking mEOS to a degron via Sortase, preserving fluorescence and enabling functional analysis.

Mesoscopic SCAPE Microscope with a Rescanned, Super-oblique Illumination Plane

This paper introduces a mesoscopic SCAPE microscope with a super-oblique light sheet and rescanning strategy, achieving high-resolution volumetric imaging over large fields of view.

Nanoscale cluster mapping of molecular complexes with pixel-based correlations

The paper introduces a method to map molecular complexes using IRIS super-resolution imaging and PC-coloring, revealing laminar complex formation in clathrin-coated structures upon EGF stimulation.

Open-Source 3D Active Sample Stabilization for Fluorescence Microscopy

This paper introduces an open-source 3D active stabilization system for fluorescence microscopy to maintain sample stability during long imaging sessions.

High-performance genetically-encoded green and red fluorescent biosensors for pyruvate

The paper develops high-performance green and red fluorescent biosensors for pyruvate with large fluorescence intensity changes and varying affinities, enabling multicolor imaging in mammalian cells.

A Beauty Dye Staining (ABDS) - inexpensive marker-ink based open-source alternative for commercial membrane vital dyes

The paper introduces ABDS, an inexpensive open-source dye for eukaryotic cell visualization, and provides its preparation method and usage examples.

High-throughput, unbiased single-molecule displacement mapping with deep learning reveals spatiotemporal heterogeneities in intracellular diffusivity

This paper introduces Hi-SMdM, a high-throughput single-molecule diffusivity microscopy approach that enables unbiased mapping of spatiotemporal heterogeneities in intracellular diffusivity with improved resolution and throughput.

A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging

The paper introduces four new monomeric fluorescent proteins for bacterial time-lapse imaging, showing improved photostability, brightness, and monomericity compared to existing standards.

One-click image reconstruction in single-molecule localization microscopy via deep learning

The paper introduces AutoDS and AutoDS3D, deep learning tools for single-molecule super-resolution microscopy that reduce manual intervention and computation time.

Curved light sheet microscopy for centimeter-scale cleared tissues imaging

The paper introduces a curved light sheet microscope for high-throughput imaging of cleared tissues, achieving diffraction-limited resolution over a large field of view.

Alpha-BET: Functional labeling of envelope glycoproteins with single domain antibodies for in-virus single molecule imaging

Alpha-BET uses AlphaFold to identify optimal sites for labeling viral glycoproteins with nanobodies, enabling high-resolution imaging of viral structures without disrupting their function.

Highly Dynamic and Sensitive NEMOer Calcium Indicators for Imaging ER Calcium Signals in Excitable Cells

The paper introduces NEMOer, a set of genetically encoded calcium indicators that enhance the detection of ER/SR calcium dynamics, enabling sensitive and dynamic imaging in both excitable and non-excitable cells.

Fast, Accurate, and Versatile Data Analysis Platform for the Quantification of Molecular Spatiotemporal Signals

AQuA2 quantifies molecular spatiotemporal signals from live imaging data by decomposing complex datasets into signaling events, demonstrating applications across biosensors and cell types.

Far-red fluorescent genetically encoded calcium ion indicators

This paper introduces two new far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, characterized for their Ca2+ sensitivity and performance in neuronal imaging.

Photoactivatable Plasma Membrane Probe Through Self-Triggered Photooxidation Cascade for Live Super-Resolution Microscopy

A novel method (RIM-Deep) enhances imaging depth and resolution stability of deep-cleared brain tissue in inverted confocal microscopy

RIM-Deep improves deep imaging of cleared brain tissue in inverted confocal microscopy, extending imaging depth from 2 mm to 5 mm in cleared macaque and mouse brains.

In-gel refolding allows fluorescence detection of fully denatured GFPs after SDS-PAGE

This paper introduces a method to refold fully denatured GFPs within SDS-PAGE gels using cyclodextrin and methanol, enabling in-gel fluorescence detection.

Improving single molecule localisation microscopy reconstruction by extending the temporal context

The paper improves single-molecule localization microscopy by extending temporal context using a hybrid U-Net and Transformer model, achieving better resolution than existing methods.

A Bayesian Solution to Count the Number of Molecules within a Diffraction Limited Spot

This paper introduces a Bayesian method to estimate the number of fluorescent emitters in diffraction-limited spots, showing improved accuracy over existing methods.