Glycosylation and Glycoproteins Research

N-Glycopedia: Libraries for Native N-glycan Structural Analysis

The paper introduces N-glycopedia, a resource of N-glycan standards and analytical methods for structural analysis, enabling more accurate glycomics research.

Type 1 LacNAc and LacdiNAc on N-glycans as molecular biomarkers of human melanoma cells

This study identifies type 1 LacNAc and LacdiNAc structures on N-glycans as biomarkers of melanoma progression by comparing melanocytes and melanoma cell lines.

B3GNT7 regulates mucin glycosylation and protects against colitis and infection

This study shows B3GNT7 regulates colonic mucus glycosylation, protects against colitis and infection, and has a distinct function from other B3GNT family members.

Iterative structural homology search identifies new substrates of the protein O-fucosyltransferases POFUT3 and POFUT4

The paper identifies new substrates of POFUT3 and POFUT4 using structural homology searches, showing MFAP2/MFAP5 are O-fucosylated and require POFUT3 for secretion.

GlycoAvatars: Bead-Coated Membrane Models for Studying the Cancer-Immune Cells Interactome

The paper introduces GlycoAvatars, bead-coated membrane models, to study glycan-mediated interactions between cancer and immune cells, identifying molecular nodes involved in immune synapses.

Siglec-G on B cells restrains the germinal center response by controlling T cell help during positive selection

This study identifies Siglec-G as a checkpoint receptor on B cells that limits germinal center (GC) response by modulating T cell help during positive selection.

Glycans in fertilization and adhesion: histochemical and ultrastructural insights from the chaetognath Spadella cephaloptera

This study identifies glycan distribution in the reproductive and attachment systems of the chaetognath Spadella cephaloptera using histochemical and ultrastructural methods.

Glycosite Mapping and in situ Mass Spectrometry Imaging of MUC2 Glycopeptides via On-slide Digestion with Mucinase StcE

This paper maps mucin glycoforms in tumors using on-tissue digestion and mass spectrometry, revealing distinct glycosylation patterns in colorectal mucinous adenocarcinomas.

Local glycan engineering induces systemic antitumor immune reactions via antigen cross-presentation

This study shows that local glycan engineering using AAV-delivered sialidases reduces tumor growth and enhances systemic antitumor immunity through improved cross-presentation and dendritic cell activation.

De-N-glycosylation of in vivo and in vitro adipogenic stem cell products unmasks differential expression of CD36 glycoprotein in human adipogenesis

The paper identifies CD36 as a key glycoprotein upregulated during adipogenesis through de-N-glycosylation and proteomics analysis of stem cells and adipocytes.

A comparative investigation of the mannose binding interface in DC-SIGN and MRC1 carbohydrate recognition domains with all-atom molecular dynamics simulations

This study uses molecular dynamics simulations to compare mannose binding in DC-SIGN and MRC1 CRDs, revealing differences in binding modes and affinity.

Application of post glycosylation modifying enzymes for mass spectrometry imaging of modified N-glycans in situ.

This paper introduces a method using post-glycosylation modifying enzymes to visualize specific PGMs in tissue N-glycans via mass spectrometry imaging.

Glycan Painting: Triplex Lectin Staining Enables Visualization of Cell-Type-Specific Glycan Profiles in Tissue Sections

This paper introduces Glycan Painting, a multiplex staining method using lectins to visualize cell-type-specific glycan profiles in tissue sections through distinct color patterns.

Dolichol biosynthesis in yeast traces the expanded reaction pathway of higher eukaryotes

This study identifies two yeast enzymes, Env9 and Tda5, that perform distinct steps in dolichol biosynthesis, replacing the dual role of DHRSX in humans.

Structural implications of glycosylation on the voltage-gated sodium channel β3-subunit

This paper investigates the structural impact of glycosylation on the β3-subunit of voltage-gated sodium channels using glycan profiling and molecular dynamics simulations.

SugarBase: mapping glycomolecule precursors in microbes

SugarBase identifies microbial nucleotide sugar profiles, revealing species-specific and previously unannotated sugars, and expands antimicrobial targets.

Replication of 10 novel loci involved in human plasma protein N-glycosylation using MALDI-MS and UHPLC-FD data

The paper replicates ten novel loci associated with human plasma N-glycosylation using MALDI-MS and UHPLC-FD data and identifies potential causal genes.

Glycosylation-Modulated Conformational Diversity in Neurotrophin Receptors

The paper uses molecular dynamics simulations to study how glycosylation affects the structure, flexibility, and neurotrophin binding of p75, TrkA, and TrkB receptors.

NovoGlyco: mapping protein glycosylation in prokaryotes

NovoGlyco identifies novel protein glycosylation in prokaryotes using shotgun proteomics data, revealing previously unreported flagella O-glycans in Campylobacter fetus.

Sialylated Gangliosides are Required for Plasma Membrane Organization and Neuronal Function

The paper shows that sialylated gangliosides are essential for plasma membrane organization and neuronal function, with ST3GAL5 deficiency causing severe disruptions in glycosphingolipid composition and neuronal activity.

Tetrahydrocurcumin Suppresses Bladder Carcinogenesis via Reprogramming O-GlcNAcylation-Phosphorylation Crosstalk

This study identifies O-GlcNAcylation and phosphorylation remodeling in bladder cancer and shows that tetrahydrocurcumin suppresses tumor growth by reprogramming these modifications.

FUT8 mediated core fucosylation modulates growth related functions of LRP1 in liver cancer cells

This study identifies LRP1 as a core-fucosylated protein regulated by FUT8 in liver cancer, showing its role in cell proliferation and signaling.

Global O-GlcNAcome Identifies O-GlcNAcylated Proteins Regulating Oligodendrocyte Precursor Cells Differentiation

The paper identifies O-GlcNAcylated proteins in OPCs and shows their role in differentiation, with Vimentin as a key target.

Molecular mechanisms of immune evasion by host protein glycosylation of a bacterial immunogen used in nucleic acid vaccines

This study identifies how host glycosylation of bacterial antigens in nucleic acid vaccines reduces immune recognition by shielding epitopes and altering antibody binding.

Extended nuclear glycosylation regulates RNA processing

The paper shows that extended O-glycans are present on intranuclear proteins and demonstrate their role in RNA processing through specific examples.

O-mannose glycosylations influence E-cadherin functional interactions

This study investigates how O-mannose glycosylations on E-cadherin affect its protein interactions, showing O-Man-dependent changes in CDH1 associations with CDH3 and other proteins.

Structure and mechanism of the human TMEM260 O-mannosyltransferase

This study determines the cryo-EM structure of human TMEM260, revealing its mechanism for O-mannosylation of key receptors and providing insights into related congenital disorders.

O-GlcNAcylation regulates PPAR-driven metabolic programming in intestinal stem cells

This study shows that O-GlcNAcylation regulates PPAR-driven metabolic programming in intestinal stem cells by linking dietary metabolism to transcriptional control.

Multi-omics definition of the sex-specific glycoproteome of murine tissues

The paper defines sex-specific glycoproteomes in mouse tissues using multi-omics integration, revealing distinct glycoform patterns and enzyme relationships across 19 tissues.

A Novel Glycoproteomics Platform for High-Throughput Identification of Disease-Associated Glycoforms

The paper introduces GDAS, a high-throughput glycoproteomics platform that identifies disease-specific glycoforms using mass spectrometry data and machine learning.