Extracellular vesicles in disease

Clinical-grade platelet-derived biomaterials mitigate mitochondrial dysfunction in preclinical models of neuronal injury

This study shows that platelet-derived biomaterials reduce mitochondrial dysfunction in neuronal injury models by restoring ATP production, reducing ROS, and improving mitochondrial structure and function.

Exploring Organ-Specific Extracellular Vesicles in Metabolic Improvements Following Bariatric Surgery in Adolescents with Obesity

This study investigates how organ-specific extracellular vesicles (sEVs) contribute to metabolic improvements after vertical sleeve gastrectomy (VSG) in adolescents with obesity, finding that liver-derived sEVs correlate with BMI and BCAAs, while adipose-derived sEVs correlate with liver function markers.

Lipid Nanoparticle Delivery of Mesenchymal Stromal Cell-Derived microRNA 187-3p as a First-in-Class Therapy for Myocardial Dysfunction in Sepsis

This paper demonstrates that lipid nanoparticle-delivered miR-187 reduces inflammation and improves cardiac function in sepsis models.

Extracellular Vesicles-Mediated Crosstalk in Bone: miR-150-5p as a Mechanosensitive Inhibitor of Osteoclastogenesis

This study identifies mechanically-activated osteocyte-derived extracellular vesicles and miR-150-5p as key regulators of osteoclastogenesis and potential therapeutic targets for skeletal diseases.

BeWo-derived extracellular vesicles downregulate IL-6Rα expression via miRNAs on CD4+ T lymphocytes

The paper shows BeWo-derived extracellular vesicles reduce IL-6Rα on CD4+ T cells via miRNAs, altering the IL-6 pathway and T cell function in vitro and correlating with pregnancy-associated IL-6R downregulation.

Stage-specific extracellular vesicle cargo from Schwann cells orchestrates peripheral nerve regeneration

This study identifies stage-specific molecular cargo in Schwann cell-derived extracellular vesicles and shows their roles in peripheral nerve regeneration.

Prostate-specific EV capture with sufficient RNA yield to enable transcriptomic profiling

The paper develops a method to specifically capture prostate-derived extracellular vesicles from urine, enabling transcriptomic analysis and improving specificity for prostate cancer detection.

Development of an EMT-related exosomal miRNA signature that can predict prognosis in hepatocellular carcinoma

The paper identifies an exosomal miRNA signature related to EMT that predicts prognosis in hepatocellular carcinoma with moderate accuracy.

VDisk: A Microfluidic Cartridge for Automated and Reproducible Label-Free Isolation of Extracellular Vesicles from Plasma

VDisk is a microfluidic platform for automated, label-free EV isolation from plasma, showing improved reproducibility and EV/contaminant ratios compared to SEC.

Proinflammatory stress activates neutral sphingomyelinase 2 based generation of a ceramide-enriched β cell EV subpopulation

This study shows that proinflammatory stress in beta cells increases ceramide-enriched extracellular vesicles via nSMase2, which may affect paracrine signaling in diabetes.

A compendium of bona fide reference markers for genuine plant-derived extracellular vesicles and their degree of phylogenetic conservation

This paper identifies and evaluates potential plant-derived extracellular vesicle (PEV) markers, analyzing their phylogenetic conservation across plant and animal species.

Extracellular vesicles released from cortical neurons influence spontaneous activity of recipient neurons.

This study shows that extracellular vesicles from cortical neurons alter spontaneous activity in recipient neurons by increasing postsynaptic potential amplitude.

Syntenin orchestrates matrix degradation by controlling MT1-MMP secretion in small extracellular vesicles and invadopodia formation

This study shows syntenin facilitates ECM degradation by controlling MT1-MMP secretion in sEV and invadopodia formation in TNBC cells.

Tumoral hypoxic extracellular vesicles foster a protective microenvironment in triple-negative breast cancer

This study investigates how hypoxic extracellular vesicles from triple-negative breast cancer cells influence the tumor microenvironment by promoting M2-like macrophages and pro-tumoral signaling.

Method for isolation of small extracellular vesicles from different biofluids and workflow for Mass Spectrometry based-shotgun proteomics and RNA isolation

The paper develops an improved ultracentrifugation method for isolating small extracellular vesicles from biofluids and demonstrates its use for proteomics and RNA isolation.

Human Decidual RUNX1 Promotes Angiogenesis and Trophoblast Differentiation by Regulating Extracellular Vesicle Signaling

This study shows that RUNX1 in human decidual cells regulates extracellular vesicle secretion and cargo proteins like ANGPTL2 and IGF2, which promote angiogenesis and trophoblast differentiation during placenta formation.

Visualizing extracellular vesicle-mediated RNA transfer using a novel metabolic labeling approach

This study introduces a metabolic labeling method using 5-EU and UCK2 overexpression to track RNA in donor cells, EVs, and recipient cells, enabling visualization and quantification of EV-RNA transfer.

Plasma exosomes from individuals with type 2 diabetes drive breast cancer aggression in patient-derived organoids

This study shows that exosomes from type 2 diabetes patients promote breast cancer aggressiveness in organoids by expanding immunosuppressive cells and increasing tumor heterogeneity and invasiveness.

Orthogonal and Robust Analytics Enable Reproducible and Scalable Manufacturing of High Purity Extracellular Vesicles Derived from Mesenchymal Stromal Cells

This paper develops an analytical toolbox and scalable downstream processing workflow for high purity extracellular vesicles from mesenchymal stromal cells, using orthogonal methods to improve reproducibility and purity.

Prediction of exosomal miRNA-based biomarkers for liquid biopsy

The paper develops models to predict exosomal miRNAs using alignment-based and AI approaches, achieving improved performance with an ensemble method.

A deep, quantitative lipid atlas of extracellular vesicles across multiple cell lines

This study quantitatively characterizes the lipid composition of extracellular vesicles from multiple cell lines to identify biomarkers and inform EV engineering.

Platelet mitochondrial transfer via extracellular vesicles modulates neutrophil phenotype and function

This study shows that platelet extracellular vesicles containing mitochondria are internalized by neutrophils, altering their activation, adhesion, migration, and phagocytic capacity.

Eukaryotic Translation Initiation Factor Loaded Extracellular Vesicles Promotes Macrophage Cholesterol Metabolism in ovarian cancer

This study shows that eIF4E-loaded extracellular vesicles from ovarian cancer promote macrophage cholesterol metabolism, enhancing immunosuppression via PD-L1 and HMGCR.

Fluorescence Characterization of Extracellular Vesicles using Single-Molecule Confocal Microscopy

This study develops a method to detect and quantify extracellular vesicles using fluorescent antibodies and single-molecule confocal microscopy, achieving femtomolar sensitivity without purification.

Assessing Extracellular Vesicle Turnover In vivo Using Highly Sensitive Phosphatidylserine-Binding Reagents

This study evaluates PS-binding reagents for detecting PS+ EVs in blood, showing MFG-E8 derivatives are more sensitive and reveal rapid clearance of PS+ EVs from circulation in mice.

Lipidomic and Proteomic Insights from Extracellular Vesicles in Postmortem Dorsolateral Prefrontal Cortex Reveal Substance Use Disorder-Induced Brain Changes

This study isolates extracellular vesicles from postmortem brain tissue and uses multi-omics analysis to identify lipidomic and proteomic changes associated with substance use disorder.

Extracellular Vesicle Gene Expression Enables Sensitive Detection of Colorectal Neoplasia

This study compares EV transcriptomics, proteomics, and cfDNA methylation for detecting colorectal neoplasia, finding EV transcriptomics most sensitive for advanced adenomas and CRC.

Development of a High-throughput Morphological Assay for Evaluating Mesenchymal Stromal Cell-derived Extracellular Vesicle Modulation of Brain Pericyte Secretory Phenotype

This study develops a high-throughput morphological assay to evaluate how MSC-EVs modulate brain pericyte secretory phenotype, showing distinct morphological responses under different manufacturing conditions.

Dual-domain Flower signaling coordinates extracellular vesicles-mediated fitness selection and cell-intrinsic survival in astrocytes

The paper identifies a dual-domain mechanism in the Flower protein that coordinates extracellular vesicle-mediated fitness selection and cell-intrinsic survival in astrocytes, with implications for Alzheimer's disease.

Cross-kingdom miRNA delivery by Panax notoginseng-derived EVs restores neuronal function after ischemic injury

This study shows that Panax notoginseng-derived extracellular vesicles deliver miRNAs to neurons, reducing ischemic injury by modulating stress responses and mitochondrial function.